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Methodical Aspects of Biogas Production in Small-Volume Bioreactors in Laboratory Investigations

Author

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  • Agnieszka Kasprzycka

    (Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, Poland)

  • Jan Kuna

    (Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, Poland)

Abstract

The aim of this study was to develop a methodology to investigate the biofermentation process in small-volume fermenters. Dark serum bottles with a volume of 100–120 mL, tightly sealed with a rubber septum, were used as bioreactors. The optimum measurement conditions in this type of bioreactor comprise: (i) filling two-thirds of the maximum volume with a suspension; (ii) a 2% bioreactor loading (on a dry basis) and; (iii) the daily equalization of pressure by removing the biogas through the septum pierced with a syringe needle and the intensive mixing of the remaining suspension. The methane yield (quantity and dynamics) obtained in this type of bioreactor is analogous to that of industrial bioreactors or large-scale laboratory bioreactors. The use of small-volume bioreactors that can be incubated will facilitate the preliminary selection of analysed systems and provide an indication of those that should be investigated in large-scale bioreactors.

Suggested Citation

  • Agnieszka Kasprzycka & Jan Kuna, 2018. "Methodical Aspects of Biogas Production in Small-Volume Bioreactors in Laboratory Investigations," Energies, MDPI, vol. 11(6), pages 1-10, May.
    • Handle: RePEc:gam:jeners:v:11:y:2018:i:6:p:1378-:d:149491
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    References listed on IDEAS

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    1. Safari, Mahmood & Abdi, Reza & Adl, Mehrdad & Kafashan, Jalal, 2018. "Optimization of biogas productivity in lab-scale by response surface methodology," Renewable Energy, Elsevier, vol. 118(C), pages 368-375.
    2. Shen, Yanwen & Linville, Jessica L. & Urgun-Demirtas, Meltem & Schoene, Robin P. & Snyder, Seth W., 2015. "Producing pipeline-quality biomethane via anaerobic digestion of sludge amended with corn stover biochar with in-situ CO2 removal," Applied Energy, Elsevier, vol. 158(C), pages 300-309.
    3. Markard, Jochen & Stadelmann, Martin & Truffer, Bernhard, 2009. "Prospective analysis of technological innovation systems: Identifying technological and organizational development options for biogas in Switzerland," Research Policy, Elsevier, vol. 38(4), pages 655-667, May.
    4. Amigun, B. & Sigamoney, R. & von Blottnitz, H., 2008. "Commercialisation of biofuel industry in Africa: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 12(3), pages 690-711, April.
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    Cited by:

    1. Luo, Tao & Khoshnevisan, Benyamin & Pan, Junting & Ge, Yihong & Mei, Zili & Xue, Jian & Fu, Yanran & Liu, Hongbin, 2020. "How exothermic characteristics of rice straw during anaerobic digestion affects net energy production," Energy, Elsevier, vol. 212(C).
    2. Spyridon Achinas & Gerrit Jan Willem Euverink, 2019. "Feasibility Study of Biogas Production from Hardly Degradable Material in Co-Inoculated Bioreactor," Energies, MDPI, vol. 12(6), pages 1-11, March.
    3. Spyridon Achinas & Gerrit Jan Willem Euverink, 2019. "Effect of Combined Inoculation on Biogas Production from Hardly Degradable Material," Energies, MDPI, vol. 12(2), pages 1-13, January.
    4. Dimitar Karakashev & Yifeng Zhang, 2018. "BioEnergy and BioChemicals Production from Biomass and Residual Resources," Energies, MDPI, vol. 11(8), pages 1-6, August.

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