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Optimizing Docosahexaenoic Acid (DHA) Production by Schizochytrium sp. Grown on Waste Glycerol

Author

Listed:
  • Natalia Kujawska

    (InnovaTree Sp. z o.o., 81-451 Gdynia, Poland)

  • Szymon Talbierz

    (InnovaTree Sp. z o.o., 81-451 Gdynia, Poland)

  • Marcin Dębowski

    (Department of Environment Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, 10-720 Olsztyn, Poland)

  • Joanna Kazimierowicz

    (Department of Water Supply and Sewage Systems, Faculty of Civil Engineering and Environmental Sciences, Bialystok University of Technology, 15-351 Bialystok, Poland)

  • Marcin Zieliński

    (Department of Environment Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, 10-720 Olsztyn, Poland)

Abstract

The aim of this study was to optimize biomass and docosahexaenoic acid (DHA) production by Schizochytrium sp. grown on waste glycerol as an organic carbon source. Parameters having a significant effect on biomass and DHA yields were screened using the fractional Plackett–Burman design and the response surface methodology (RSM). Schizochytrium sp. growth was most significantly influenced by crude glycerin concentration in the growth medium (150 g/dm 3 ), process temperature (27 °C), oxygen in the bioreactor (49.99% v / v ), and the concentration of peptone as a source of nitrogen (9.99 g/dm 3 ). The process parameter values identified as optimal for producing high DHA concentrations in the biomass were as follows: glycerin concentration 149.99 g/dm 3 , temperature 26 °C, oxygen concentration 30% ( v / v ), and peptone concentration 2.21 g/dm 3 . The dry cell weight (DCW) obtained under actual laboratory conditions was 66.69 ± 0.66 g/dm 3 , i.e., 1.27% lower than the predicted value. The DHA concentration obtained in the actual culture was at 17.25 ± 0.33 g/dm 3 , which was 3.03% lower than the predicted value. The results obtained suggest that a two-step culture system should be employed, with the first phase focused on high production of Schizochytrium sp. biomass, and the second focused on increasing DHA concentration in the cells.

Suggested Citation

  • Natalia Kujawska & Szymon Talbierz & Marcin Dębowski & Joanna Kazimierowicz & Marcin Zieliński, 2021. "Optimizing Docosahexaenoic Acid (DHA) Production by Schizochytrium sp. Grown on Waste Glycerol," Energies, MDPI, vol. 14(6), pages 1-17, March.
    • Handle: RePEc:gam:jeners:v:14:y:2021:i:6:p:1685-:d:519525
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    References listed on IDEAS

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    2. Bwapwa, Joseph K. & Anandraj, Akash & Trois, Cristina, 2017. "Possibilities for conversion of microalgae oil into aviation fuel: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 80(C), pages 1345-1354.
    3. Liu, Xiaoyan & Zhu, Fenfen & Zhang, Rongyan & Zhao, Luyao & Qi, Juanjuan, 2021. "Recent progress on biodiesel production from municipal sewage sludge," Renewable and Sustainable Energy Reviews, Elsevier, vol. 135(C).
    4. Saifuddin Nomanbhay & Mei Yin Ong & Kit Wayne Chew & Pau-Loke Show & Man Kee Lam & Wei-Hsin Chen, 2020. "Organic Carbonate Production Utilizing Crude Glycerol Derived as By-Product of Biodiesel Production: A Review," Energies, MDPI, vol. 13(6), pages 1-23, March.
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    Cited by:

    1. Marcin Dębowski & Izabela Świca & Joanna Kazimierowicz & Marcin Zieliński, 2022. "Large Scale Microalgae Biofuel Technology—Development Perspectives in Light of the Barriers and Limitations," Energies, MDPI, vol. 16(1), pages 1-23, December.
    2. Shunli Feng & Yihan Guo & Yulu Ran & Qingzhuoma Yang & Xiyue Cao & Huahao Yang & Yu Cao & Qingrui Xu & Dairong Qiao & Hui Xu & Yi Cao, 2023. "Production of Microbial Lipids by Saitozyma podzolica Zwy2-3 Using Corn Straw Hydrolysate, the Analysis of Lipid Composition, and the Prediction of Biodiesel Properties," Energies, MDPI, vol. 16(18), pages 1-22, September.
    3. Natalia Kujawska & Szymon Talbierz & Marcin Dębowski & Joanna Kazimierowicz & Marcin Zieliński, 2021. "Cultivation Method Effect on Schizochytrium sp. Biomass Growth and Docosahexaenoic Acid (DHA) Production with the Use of Waste Glycerol as a Source of Organic Carbon," Energies, MDPI, vol. 14(10), pages 1-16, May.
    4. Marcin Zieliński & Marcin Dębowski & Joanna Kazimierowicz & Izabela Świca, 2023. "Microalgal Carbon Dioxide (CO 2 ) Capture and Utilization from the European Union Perspective," Energies, MDPI, vol. 16(3), pages 1-27, February.

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