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Production of Microbial Lipids by Saitozyma podzolica Zwy2-3 Using Corn Straw Hydrolysate, the Analysis of Lipid Composition, and the Prediction of Biodiesel Properties

Author

Listed:
  • Shunli Feng

    (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu 610065, China)

  • Yihan Guo

    (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu 610065, China)

  • Yulu Ran

    (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu 610065, China)

  • Qingzhuoma Yang

    (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu 610065, China)

  • Xiyue Cao

    (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu 610065, China)

  • Huahao Yang

    (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu 610065, China)

  • Yu Cao

    (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu 610065, China)

  • Qingrui Xu

    (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu 610065, China)

  • Dairong Qiao

    (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu 610065, China)

  • Hui Xu

    (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu 610065, China)

  • Yi Cao

    (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu 610065, China)

Abstract

Although Saitozyma podzolica Zwy2-3 can use the enzymatic hydrolysate of corn stalks treated with an ammonium carbonate-steam explosion (EHCS-ACSE) as a substrate for lipid accumulation, the inefficient conversion of sugars from EHCS-ACSE into lipids necessitates the further optimization of fermentation parameters. Response surface design was used to optimize the primary fermentation parameters. Under the optimized conditions of the reducing sugar concentration of 89.44 g/L, yeast extract concentration of 3.88 g/L, rotational speed of 219 rpm, and incubation time of 122 h, the maximum lipid production achieved 11.45 g/L, which was 2.28 times higher than the results of the previous study. In addition, lipid profiling showed the presence of four fatty acid methyl esters, with the highest percentage being 61.84% oleic acid, followed by 21.53% palmitic acid, 13.05% stearic acid, and 3.58% linoleic acid. It is noteworthy that the composition and relative abundance of microbial lipids remained constant under different culture conditions. The characteristics of Zwy2-3 biodiesel, such as the iodine value (62.09), cetane number (59.29), density (0.87 g/cm 3 ), and oxidation stability (35.53), meet the international standards (ASTM D6751-02 and EN 14214) for biodiesel. The present study further demonstrated that S. podzolica Zwy2-3 can efficiently utilize EHCS-ACSE for microbial lipid accumulation, and its lipids have favorable qualities that make them suitable for biodiesel production.

Suggested Citation

  • Shunli Feng & Yihan Guo & Yulu Ran & Qingzhuoma Yang & Xiyue Cao & Huahao Yang & Yu Cao & Qingrui Xu & Dairong Qiao & Hui Xu & Yi Cao, 2023. "Production of Microbial Lipids by Saitozyma podzolica Zwy2-3 Using Corn Straw Hydrolysate, the Analysis of Lipid Composition, and the Prediction of Biodiesel Properties," Energies, MDPI, vol. 16(18), pages 1-22, September.
    • Handle: RePEc:gam:jeners:v:16:y:2023:i:18:p:6630-:d:1240399
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    References listed on IDEAS

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