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Biodiesel production via simultaneous esterification and transesterification of Periplaneta americana oil with liquid lipase Eversa® transform 2.0

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  • Guo, Jing-jing
  • Gao, Shuai
  • Yang, Jian
  • Zhang, Huan
  • Wang, Yi-tong
  • Ding, Wo-na
  • Fang, Zhen

Abstract

Undeveloped Periplaneta americana oil (acid value 38.32 mg KOH/g) was directly used for one-step production of biodiesel with lipase without acid-pretreatment step for the commercial alkaline process. Biodiesel was produced via simultaneous esterification and transesterification of Periplaneta americana oil in the presence of lipase Eversa® Transform 2.0 (ET2) (12 US$/kg) in solvent-free system. The maximum biodiesel yield of 98.63 % was obtained under the optimized conditions of 32 °C, 8.5 wt.% lipase dosage, 8 h, 6.5/1 methanol/oil molar ratio, and 4 wt.% water. Lipase ET2 was recycled 6 times at > 89.52 % biodiesel yield. Biodiesel yield of 93.94 % was further achieved in a 1 L reactor with 15.08 g/kg lipase/biodiesel. Biodiesel cost was estimated as 589.3 US$/ton. Kinetics study gave activation energy of 24.50 kJ/mol with kinetic Michaelis constant of 1.19 mol/L. The physicochemical properties of biodiesel met both Chinese national and US ASTM standards that could be blended with petro-diesel to be applied in both countries. This study suggests that lipase could directly catalyze waste oils for the production of biodiesel at low temperature.

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  • Guo, Jing-jing & Gao, Shuai & Yang, Jian & Zhang, Huan & Wang, Yi-tong & Ding, Wo-na & Fang, Zhen, 2024. "Biodiesel production via simultaneous esterification and transesterification of Periplaneta americana oil with liquid lipase Eversa® transform 2.0," Renewable Energy, Elsevier, vol. 229(C).
  • Handle: RePEc:eee:renene:v:229:y:2024:i:c:s0960148124008243
    DOI: 10.1016/j.renene.2024.120756
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