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Optimization of process variables for the production of biodiesel by transesterification of used cooking oil using lipase from Nile tilapia viscera

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  • Patchimpet, Jaran
  • Simpson, Benjamin K.
  • Sangkharak, Kanokphorn
  • Klomklao, Sappasith

Abstract

Biodiesel synthesis through transesterification of used cooking oil or frying oils using the viscera lipase from Nile tilapia (Oreochromis niloticus) as a low-cost feedstock and catalyst, respectively, was optimized. The influences of operating factors, including methanol/oil molar ratio, enzyme loading, reaction time, type of alcohol, water content and reaction temperature on the yield of biodiesel were investigated. The optimized conditions to achieve maximum biodiesel yield were obtained using an enzyme loading of 30 kUnit, a methanol to oil molar ratio of 4:1, a water content of 3%, a reaction temperature of 45 °C and a 28 h reaction time. Under these optimal operating conditions, the highest biodiesel yield observed was 96.5%. The attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) was applied to ensure the conversion of used cooking oil into biodiesel. The biodiesel characteristics met the specifications set as prescribed by EN 14214 and ASTM D 6751. The results from this investigation show the viability of economical biodiesel production using byproducts as both source and catalyst.

Suggested Citation

  • Patchimpet, Jaran & Simpson, Benjamin K. & Sangkharak, Kanokphorn & Klomklao, Sappasith, 2020. "Optimization of process variables for the production of biodiesel by transesterification of used cooking oil using lipase from Nile tilapia viscera," Renewable Energy, Elsevier, vol. 153(C), pages 861-869.
  • Handle: RePEc:eee:renene:v:153:y:2020:i:c:p:861-869
    DOI: 10.1016/j.renene.2020.02.039
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    3. Ezzati, Rohollah & Ranjbar, Shahram & Soltanabadi, Azim, 2021. "Kinetics models of transesterification reaction for biodiesel production: A theoretical analysis," Renewable Energy, Elsevier, vol. 168(C), pages 280-296.
    4. Khozeymeh Nezhad, Marziyeh & Aghaei, Hamidreza, 2021. "Tosylated cloisite as a new heterofunctional carrier for covalent immobilization of lipase and its utilization for production of biodiesel from waste frying oil," Renewable Energy, Elsevier, vol. 164(C), pages 876-888.
    5. Zamani, Ali Salehi & Saidi, Majid & Najafabadi, Ali Taheri, 2023. "Selective production of diesel-like alkanes via Neem seed oil hydrodeoxygenation over Ni/MgSiO3 catalyst," Renewable Energy, Elsevier, vol. 209(C), pages 462-470.
    6. Iuliano, Mariagrazia & Sarno, Maria & De Pasquale, Salvatore & Ponticorvo, Eleonora, 2020. "Candida rugosa lipase for the biodiesel production from renewable sources," Renewable Energy, Elsevier, vol. 162(C), pages 124-133.

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