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Biochar-Enhanced Nitrogen Removal in SBBR Under PFOA Stress: The Role of Quorum Sensing

Author

Listed:
  • Zhiqi Lu

    (Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, China)

  • Mengzhe Zhao

    (Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, China)

  • Xianglong He

    (Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, China)

  • Hongjing Li

    (Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, China)

Abstract

Perfluorooctanoic acid (PFOA), an emerging organic contaminant frequently detected in wastewater, inhibits biological nitrogen removal processes, posing challenges to sustainable wastewater treatment. Mitigating the adverse effects of PFOA while enhancing total nitrogen (TN) removal efficiency remains a critical concern. In this study, three sequencing batch biofilm reactors (SBBRs) were operated under low-oxygen conditions with a C/N ratio of 4.0 to investigate enhanced nitrogen removal under PFOA stress using biochar. Compared to the 78.1% TN removal efficiency in the control reactor (SBBR-0) with an initial TN concentration of 50 mg/L, the addition of PFOA decreased TN removal by 2.3% in SBBR-1, while the combined addition of PFOA and biochar increased it by 3.2% in SBBR-2. Biochar, acting through its electron-donating surface functional groups, mitigated PFOA-induced reactive oxygen species accumulation and increased adenosine triphosphate production. These effects promoted the generation of quorum sensing (QS) signaling molecules, facilitating microbial communication and cooperation. Consequently, the relative abundance of key nitrogen-removing bacteria, such as Thauera (from 7.90% to 9.92%) and Nitrosomonas (from 1.42% to 5.75%), increased, leading to enhanced nitrogen removal efficiency. A metagenomic analysis revealed that biochar significantly reduced the production of antibiotic resistance genes without promoting their dissemination. These findings provide new insights into mitigating the negative effects of PFOA and improving TN removal through QS promotion, offering a potential approach for enhancing the sustainability of wastewater treatment systems.

Suggested Citation

  • Zhiqi Lu & Mengzhe Zhao & Xianglong He & Hongjing Li, 2025. "Biochar-Enhanced Nitrogen Removal in SBBR Under PFOA Stress: The Role of Quorum Sensing," Sustainability, MDPI, vol. 17(8), pages 1-21, April.
    • Handle: RePEc:gam:jsusta:v:17:y:2025:i:8:p:3359-:d:1631443
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    References listed on IDEAS

    as
    1. Marvin Whiteley & Stephen P. Diggle & E. Peter Greenberg, 2017. "Progress in and promise of bacterial quorum sensing research," Nature, Nature, vol. 551(7680), pages 313-320, November.
    2. ChaoQing Yu & Xiao Huang & Han Chen & H. Charles J. Godfray & Jonathon S. Wright & Jim W. Hall & Peng Gong & ShaoQiang Ni & ShengChao Qiao & GuoRui Huang & YuChen Xiao & Jie Zhang & Zhao Feng & XiaoTa, 2019. "Managing nitrogen to restore water quality in China," Nature, Nature, vol. 567(7749), pages 516-520, March.
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