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Integration of Aquaculture Wastewater Treatment and Chlorella vulgaris Cultivation as a Sustainable Method for Biofuel Production

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  • Marcin Zieliński

    (Department of Environment Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Str. Oczapowskiego 5, 10-719 Olsztyn, Poland)

  • Marta Kisielewska

    (Department of Environment Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Str. Oczapowskiego 5, 10-719 Olsztyn, Poland)

  • Annamaria Talpalaru

    (Faculty of Chemical Engineering and Environmental Protection “Cristofor Simionescu”, “Gheorghe Asachi” Technical University of Iasi, 70005 Iaşi, Romania)

  • Paulina Rusanowska

    (Department of Environment Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Str. Oczapowskiego 5, 10-719 Olsztyn, Poland)

  • Joanna Kazimierowicz

    (Department of Water Supply and Sewage Systems, Faculty of Civil Engineering and Environmental Sciences, Bialystok University of Technology, 15-351 Bialystok, Poland)

  • Marcin Dębowski

    (Department of Environment Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Str. Oczapowskiego 5, 10-719 Olsztyn, Poland)

Abstract

The integration of microalgae cultivation in the treatment of aquaculture wastewater (AWW) offers a sustainable solution for the recovery of nutrients and the valorisation of biomass. In this study, the potential of Chlorella vulgaris for growth in raw AWW and its variants was investigated and the efficiency of nutrient removal, biochemical composition of biomass, biodiesel potential by FAME analysis, and biogas production were evaluated. C. vulgaris was cultivated in three media: raw AWW, microelement-enriched AWW, and a synthetic base medium. Raw AWW allowed for the highest biomass production (2.4 g VS/L) and nutrient removal efficiency (ammonia: 100%, phosphate: 93.7%, nitrate: 37.8%). The addition of microelements did not significantly improve growth or nutrient uptake. The biomass grown on AWW showed a favourable lipid profile for biodiesel, dominated by C16:0 and C18:1. The highest biogas and methane yields were recorded for biomass from raw AWW as 358 ± 11 L/kg VS and 216 ± 7 L/kg VS, respectively. The results confirm that AWW is a suitable medium for the cultivation of C. vulgaris , enabling efficient wastewater treatment and the production of high-quality biomass.

Suggested Citation

  • Marcin Zieliński & Marta Kisielewska & Annamaria Talpalaru & Paulina Rusanowska & Joanna Kazimierowicz & Marcin Dębowski, 2025. "Integration of Aquaculture Wastewater Treatment and Chlorella vulgaris Cultivation as a Sustainable Method for Biofuel Production," Energies, MDPI, vol. 18(16), pages 1-23, August.
    • Handle: RePEc:gam:jeners:v:18:y:2025:i:16:p:4352-:d:1725107
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    References listed on IDEAS

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    1. Magda Dudek & Marcin Dębowski & Joanna Kazimierowicz & Marcin Zieliński & Piera Quattrocelli & Anna Nowicka, 2022. "The Cultivation of Biohydrogen-Producing Tetraselmis subcordiformis Microalgae as the Third Stage of Dairy Wastewater Aerobic Treatment System," Sustainability, MDPI, vol. 14(19), pages 1-17, September.
    2. Bipasha Chakrabarty & Łukasz Barczak & Paulina Rusanowska & Magda Dudek & Marcin Zieliński, 2024. "Characterization of Lipid Production in Chlorella sp. Cultivated in Different Plant Fertilizers," Energies, MDPI, vol. 17(23), pages 1-14, December.
    3. Aleksandra Szaja & Agnieszka Montusiewicz & Magdalena Lebiocka, 2023. "Variability of Micro- and Macro-Elements in Anaerobic Co-Digestion of Municipal Sewage Sludge and Food Industrial By-Products," IJERPH, MDPI, vol. 20(7), pages 1-16, April.
    4. Alvin B. Culaba & Aristotle T. Ubando & Phoebe Mae L. Ching & Wei-Hsin Chen & Jo-Shu Chang, 2020. "Biofuel from Microalgae: Sustainable Pathways," Sustainability, MDPI, vol. 12(19), pages 1-19, September.
    5. Nilay Kumar Sarker & Prasad Kaparaju, 2024. "Microalgal Bioeconomy: A Green Economy Approach Towards Achieving Sustainable Development Goals," Sustainability, MDPI, vol. 16(24), pages 1-45, December.
    6. Giovanna Salbitani & Simona Carfagna, 2021. "Ammonium Utilization in Microalgae: A Sustainable Method for Wastewater Treatment," Sustainability, MDPI, vol. 13(2), pages 1-17, January.
    7. Marcin Zieliński & Marcin Dębowski & Joanna Kazimierowicz, 2022. "Outflow from a Biogas Plant as a Medium for Microalgae Biomass Cultivation—Pilot Scale Study and Technical Concept of a Large-Scale Installation," Energies, MDPI, vol. 15(8), pages 1-18, April.
    8. Chamkalani, A. & Zendehboudi, S. & Rezaei, N. & Hawboldt, K., 2020. "A critical review on life cycle analysis of algae biodiesel: current challenges and future prospects," Renewable and Sustainable Energy Reviews, Elsevier, vol. 134(C).
    9. Marta Kisielewska & Marcin Zieliński & Marcin Dębowski & Joanna Kazimierowicz & Zdzisława Romanowska-Duda & Magda Dudek, 2020. "Effectiveness of Scenedesmus sp. Biomass Grow and Nutrients Removal from Liquid Phase of Digestates," Energies, MDPI, vol. 13(6), pages 1-11, March.
    10. Marcin Zieliński & Łukasz Barczak & Paulina Rusanowska & Joanna Kazimierowicz & Marcin Dębowski, 2024. "Comparison of Biohydrogen Production by Tetraselmis subcordiformis During Cultivation Using Soil-Less Agricultural Wastewater and Effluent from Microbial Fuel Cells," Energies, MDPI, vol. 17(21), pages 1-15, October.
    11. Marcin Zieliński & Marcin Dębowski & Joanna Kazimierowicz & Izabela Świca, 2023. "Microalgal Carbon Dioxide (CO 2 ) Capture and Utilization from the European Union Perspective," Energies, MDPI, vol. 16(3), pages 1-27, February.
    12. Montingelli, M.E. & Tedesco, S. & Olabi, A.G., 2015. "Biogas production from algal biomass: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 43(C), pages 961-972.
    13. Prajapati, Sanjeev Kumar & Malik, Anushree & Vijay, Virendra Kumar, 2014. "Comparative evaluation of biomass production and bioenergy generation potential of Chlorella spp. through anaerobic digestion," Applied Energy, Elsevier, vol. 114(C), pages 790-797.
    14. Raquel Iglesias & Raúl Muñoz & María Polanco & Israel Díaz & Ana Susmozas & Antonio D. Moreno & María Guirado & Nely Carreras & Mercedes Ballesteros, 2021. "Biogas from Anaerobic Digestion as an Energy Vector: Current Upgrading Development," Energies, MDPI, vol. 14(10), pages 1-30, May.
    15. Małgorzata Hawrot-Paw & Adam Koniuszy & Patryk Ratomski & Magdalena Sąsiadek & Andrzej Gawlik, 2023. "Biogas Production from Arthrospira platensis Biomass," Energies, MDPI, vol. 16(10), pages 1-12, May.
    16. Elena Rossi & Isabella Pecorini & Renato Iannelli, 2022. "Multilinear Regression Model for Biogas Production Prediction from Dry Anaerobic Digestion of OFMSW," Sustainability, MDPI, vol. 14(8), pages 1-17, April.
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