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Fermentation of Sugar Beet Pulp by E. coli for Enhanced Biohydrogen and Biomass Production

Author

Listed:
  • Gayane Mikoyan

    (Scientific-Research Institute of Biology, Yerevan State University, 1 A. Manoogian str., Yerevan 0025, Armenia
    Microbial Biotechnologies and Biofuel Innovation Center, Yerevan State University, 1 A. Manoogian str., Yerevan 0025, Armenia)

  • Liana Vanyan

    (Scientific-Research Institute of Biology, Yerevan State University, 1 A. Manoogian str., Yerevan 0025, Armenia
    Microbial Biotechnologies and Biofuel Innovation Center, Yerevan State University, 1 A. Manoogian str., Yerevan 0025, Armenia)

  • Akerke Toleugazykyzy

    (Department of Food Technology and Processing Products, Technical Faculty, S. Seifullin Kazakh Agrotechnical Research University, 62 Zhenis Avenue, Astana 010011, Kazakhstan)

  • Roza Bekbayeva

    (Department of Food Technology and Processing Products, Technical Faculty, S. Seifullin Kazakh Agrotechnical Research University, 62 Zhenis Avenue, Astana 010011, Kazakhstan
    Department of Technological Equipment, Shakarim University, st., Glinka, 20A, Semey 071410, Kazakhstan)

  • Kamila Baichiyeva

    (Department of Food Technology and Processing Products, Technical Faculty, S. Seifullin Kazakh Agrotechnical Research University, 62 Zhenis Avenue, Astana 010011, Kazakhstan)

  • Kairat Bekbayev

    (Department of Food Technology and Processing Products, Technical Faculty, S. Seifullin Kazakh Agrotechnical Research University, 62 Zhenis Avenue, Astana 010011, Kazakhstan
    Department of Technological Equipment, Shakarim University, st., Glinka, 20A, Semey 071410, Kazakhstan)

  • Karen Trchounian

    (Scientific-Research Institute of Biology, Yerevan State University, 1 A. Manoogian str., Yerevan 0025, Armenia
    Microbial Biotechnologies and Biofuel Innovation Center, Yerevan State University, 1 A. Manoogian str., Yerevan 0025, Armenia
    Department of Biochemistry, Microbiology and Biotechnology, Faculty of Biology, Yerevan State University, 1 A. Manoogian str., Yerevan 0025, Armenia)

Abstract

This study investigates the potential of sugar beet pulp (SBP), a lignocellulosic by-product of sugar production, as a low-cost substrate for biohydrogen and biomass generation using Escherichia coli under dark fermentation conditions. Two strains—BW25113 wild-type and a genetically engineered septuple mutant—were employed. SBP was pretreated via thermochemical hydrolysis, and the effects of substrate concentration, dilution, and glycerol supplementation were evaluated. Hydrogen production was highly dependent on substrate dilution and nutrient balance. The septuple mutant achieved the highest H 2 yield in 30 g L −1 SBP hydrolysate (0.75% sulfuric acid) at 5× dilution with glycerol, reaching 12.06 mmol H 2 (g sugar) −1 and 0.28 mmol H 2 (g waste) −1 , while the wild type under the same conditions yielded 3.78 mmol H 2 (g sugar) −1 and 0.25 mmol H 2 (g waste) −1 . In contrast, undiluted hydrolysates favored biomass accumulation over H 2 production, with the highest biomass yield (0.3 g CDW L −1 ) obtained using the septuple mutant in 30 g L −1 SBP hydrolysate without glycerol. These findings highlight the potential of genetically optimized E. coli and optimized hydrolysate conditions to enhance the valorization of agro-industrial waste, supporting future advances in sustainable hydrogen bioeconomy and integrated waste biorefineries.

Suggested Citation

  • Gayane Mikoyan & Liana Vanyan & Akerke Toleugazykyzy & Roza Bekbayeva & Kamila Baichiyeva & Kairat Bekbayev & Karen Trchounian, 2025. "Fermentation of Sugar Beet Pulp by E. coli for Enhanced Biohydrogen and Biomass Production," Energies, MDPI, vol. 18(10), pages 1-15, May.
  • Handle: RePEc:gam:jeners:v:18:y:2025:i:10:p:2648-:d:1660266
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