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Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology

Author

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  • Abdi Hanra Sebayang

    (Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia
    Department of Mechanical Engineering, Medan State Polytechnic, Medan 20155, Indonesia)

  • Masjuki Haji Hassan

    (Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia)

  • Hwai Chyuan Ong

    (Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia)

  • Surya Dharma

    (Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia
    Department of Mechanical Engineering, Medan State Polytechnic, Medan 20155, Indonesia)

  • Arridina Susan Silitonga

    (Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia
    Department of Mechanical Engineering, Medan State Polytechnic, Medan 20155, Indonesia)

  • Fitranto Kusumo

    (Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia)

  • Teuku Meurah Indra Mahlia

    (Department of Mechanical Engineering, Universiti Tenaga Nasional, Kajang 43000, Selangor, Malaysia)

  • Aditiya Harjon Bahar

    (Department of Mechanical Engineering, Faculty of Engineering, The University of Melbourne, Parkville, VIC 3010, Australia)

Abstract

Bioethanol is known as a viable alternative fuel to solve both energy and environmental crises. This study used response surface methodology based on the Box-Behnken experimental design to obtain the optimum conditions for and quality of bioethanol production. Enzymatic hydrolysis optimization was performed with selected hydrolysis parameters, including substrate loading, stroke speed, α-amylase concentration and amyloglucosidase concentration. From the experiment, the resulting optimum conditions are 23.88% ( w / v ) substrate loading, 109.43 U/g α-amylase concentration, 65.44 U/mL amyloglucosidase concentration and 74.87 rpm stroke speed, which yielded 196.23 g/L reducing sugar. The fermentation process was also carried out, with a production value of 0.45 g ethanol/g reducing sugar, which is equivalent to 88.61% of ethanol yield after fermentation by using Saccharomyces cerevisiae ( S. cerevisiae ). The physical and chemical properties of the produced ethanol are within the specifications of the ASTM D4806 standard. The good quality of ethanol produced from this study indicates that Manihot glaziovii ( M. glaziovii ) has great potential as bioethanol feedstock.

Suggested Citation

  • Abdi Hanra Sebayang & Masjuki Haji Hassan & Hwai Chyuan Ong & Surya Dharma & Arridina Susan Silitonga & Fitranto Kusumo & Teuku Meurah Indra Mahlia & Aditiya Harjon Bahar, 2017. "Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology," Energies, MDPI, vol. 10(1), pages 1-13, January.
    • Handle: RePEc:gam:jeners:v:10:y:2017:i:1:p:35-:d:86686
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    References listed on IDEAS

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    1. Chi-Yang Yu & Bo-Hong Jiang & Kow-Jen Duan, 2013. "Production of Bioethanol from Carrot Pomace Using the Thermotolerant Yeast Kluyveromyces marxianus," Energies, MDPI, vol. 6(3), pages 1-8, March.
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    5. Qi Zhang & Zhenzhen Shi & Pengfei Zhang & Meng Zhang & Zhichao Li & Xi Chen & Jiping Zhou, 2018. "Ultrasonic-Assisted Pelleting of Sorghum Stalk: Predictive Models for Pellet Density and Durability Using Multiple Response Surface Methodology," Energies, MDPI, vol. 11(5), pages 1-18, May.
    6. Rafał Łukajtis & Piotr Rybarczyk & Karolina Kucharska & Donata Konopacka-Łyskawa & Edyta Słupek & Katarzyna Wychodnik & Marian Kamiński, 2018. "Optimization of Saccharification Conditions of Lignocellulosic Biomass under Alkaline Pre-Treatment and Enzymatic Hydrolysis," Energies, MDPI, vol. 11(4), pages 1-27, April.
    7. Chohan, Naseeha A. & Aruwajoye, G.S. & Sewsynker-Sukai, Y. & Gueguim Kana, E.B., 2020. "Valorisation of potato peel wastes for bioethanol production using simultaneous saccharification and fermentation: Process optimization and kinetic assessment," Renewable Energy, Elsevier, vol. 146(C), pages 1031-1040.
    8. Sarocha Pradyawong & Ankita Juneja & Muhammad Bilal Sadiq & Athapol Noomhorm & Vijay Singh, 2018. "Comparison of Cassava Starch with Corn as a Feedstock for Bioethanol Production," Energies, MDPI, vol. 11(12), pages 1-11, December.

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