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Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid ethyl esters, an advanced biofuel, by eliminating non-essential fatty acid utilization pathways

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  • Valle-Rodríguez, Juan Octavio
  • Shi, Shuobo
  • Siewers, Verena
  • Nielsen, Jens

Abstract

Microbial production of fatty acid derived chemicals and fuels is currently of great interest due to the limited resources and increasing prices of petroleum and petroleum-based products. The development of Saccharomyces cerevisiae as a fatty acid ethyl ester (FAEE) cell factory would represent an opportunity for biodiesel production due to its successful history in the biotechnology area. However, fatty acid (FA) biosynthesis is highly regulated and usually not high enough for developing an efficient production process. In S. cerevisiae, FAs are degraded by β-oxidation and a large fraction is utilized to synthesize steryl esters (SEs) and triacylglycerols (TAGs), which are not essential for the cell. Here, by eliminating non-essential FA utilization pathways, we developed a metabolic engineering strategy resulting in a S. cerevisiae strain that can overproduce FAs and in turn use these for producing FAEEs (biodiesel). Compared to the wild-type, there is an about 3-fold increase in free FA content in a strain devoid of both TAG and SE formation, a 4-fold increase in free FA content in a strain that is incapable of β-oxidation, and a 5-fold increase of free FAs in a strain lacking all of these non-essential FA utilization pathways. It is also demonstrated that there are similar positive effects on FAEE production in these deletion strains. The highest production of FAEEs is 17.2mg/l in the strain in which all these pathways were blocked. The results of this study serve as a basis for further strategies to improve the production of FA derivatives in S. cerevisiae.

Suggested Citation

  • Valle-Rodríguez, Juan Octavio & Shi, Shuobo & Siewers, Verena & Nielsen, Jens, 2014. "Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid ethyl esters, an advanced biofuel, by eliminating non-essential fatty acid utilization pathways," Applied Energy, Elsevier, vol. 115(C), pages 226-232.
    • Handle: RePEc:eee:appene:v:115:y:2014:i:c:p:226-232
    DOI: 10.1016/j.apenergy.2013.10.003
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    References listed on IDEAS

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    1. Santori, Giulio & Di Nicola, Giovanni & Moglie, Matteo & Polonara, Fabio, 2012. "A review analyzing the industrial biodiesel production practice starting from vegetable oil refining," Applied Energy, Elsevier, vol. 92(C), pages 109-132.
    2. Qiu, Fengxian & Li, Yihuai & Yang, Dongya & Li, Xiaohua & Sun, Ping, 2011. "Biodiesel production from mixed soybean oil and rapeseed oil," Applied Energy, Elsevier, vol. 88(6), pages 2050-2055, June.
    3. Eric J. Steen & Yisheng Kang & Gregory Bokinsky & Zhihao Hu & Andreas Schirmer & Amy McClure & Stephen B. del Cardayre & Jay D. Keasling, 2010. "Microbial production of fatty-acid-derived fuels and chemicals from plant biomass," Nature, Nature, vol. 463(7280), pages 559-562, January.
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    Cited by:

    1. Christwardana, Marcelinus & Frattini, Domenico & Accardo, Grazia & Yoon, Sung Pil & Kwon, Yongchai, 2018. "Early-stage performance evaluation of flowing microbial fuel cells using chemically treated carbon felt and yeast biocatalyst," Applied Energy, Elsevier, vol. 222(C), pages 369-382.
    2. Christwardana, Marcelinus & Frattini, Domenico & Duarte, Kimberley D.Z. & Accardo, Grazia & Kwon, Yongchai, 2019. "Carbon felt molecular modification and biofilm augmentation via quorum sensing approach in yeast-based microbial fuel cells," Applied Energy, Elsevier, vol. 238(C), pages 239-248.
    3. Das, Manali & Patra, Pradipta & Ghosh, Amit, 2020. "Metabolic engineering for enhancing microbial biosynthesis of advanced biofuels," Renewable and Sustainable Energy Reviews, Elsevier, vol. 119(C).

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