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DNA Methylation Profiles of the Brain-Derived Neurotrophic Factor (BDNF) Gene as a Potent Diagnostic Biomarker in Major Depression

Author

Listed:
  • Manabu Fuchikami
  • Shigeru Morinobu
  • Masahiro Segawa
  • Yasumasa Okamoto
  • Shigeto Yamawaki
  • Norio Ozaki
  • Takeshi Inoue
  • Ichiro Kusumi
  • Tsukasa Koyama
  • Kounosuke Tsuchiyama
  • Takeshi Terao

Abstract

Major depression, because of its recurring and life-threatening nature, is one of the top 10 diseases for global disease burden. Major depression is still diagnosed on the basis of clinical symptoms in patients. The search for specific biological markers is of great importance to advance the method of diagnosis for depression. We examined the methylation profile of 2 CpG islands (I and IV) at the promoters of the brain-derived neurotrophic factor (BDNF) gene, which is well known to be involved in the pathophysiology of depression. We analyzed genomic DNA from peripheral blood of 20 Japanese patients with major depression and 18 healthy controls to identify an appropriate epigenetic biomarker to aid in the establishment of an objective system for the diagnosis of depression. Methylation rates at each CpG unit was measured using a MassArray® system (SEQUENOM), and 2-dimensional hierarchical clustering analyses were undertaken to determine the validity of these methylation profiles as a diagnostic biomarker. Analyses of the dendrogram from methylation profiles of CpG I, but not IV, demonstrated that classification of healthy controls and patients at the first branch completely matched the clinical diagnosis. Despite the small number of subjects, our results indicate that classification based on the DNA methylation profiles of CpG I of the BDNF gene may be a valuable diagnostic biomarker for major depression.

Suggested Citation

  • Manabu Fuchikami & Shigeru Morinobu & Masahiro Segawa & Yasumasa Okamoto & Shigeto Yamawaki & Norio Ozaki & Takeshi Inoue & Ichiro Kusumi & Tsukasa Koyama & Kounosuke Tsuchiyama & Takeshi Terao, 2011. "DNA Methylation Profiles of the Brain-Derived Neurotrophic Factor (BDNF) Gene as a Potent Diagnostic Biomarker in Major Depression," PLOS ONE, Public Library of Science, vol. 6(8), pages 1-7, August.
    • Handle: RePEc:plo:pone00:0023881
    DOI: 10.1371/journal.pone.0023881
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    Cited by:

    1. Francesca Pirini & Elisa Guida & Fahcina Lawson & Andrea Mancinelli & Rafael Guerrero-Preston, 2015. "Nuclear and Mitochondrial DNA Alterations in Newborns with Prenatal Exposure to Cigarette Smoke," IJERPH, MDPI, vol. 12(2), pages 1-21, January.
    2. Dana E. Lauterstein & Pamella B. Tijerina & Kevin Corbett & Betul Akgol Oksuz & Steven S. Shen & Terry Gordon & Catherine B. Klein & Judith T. Zelikoff, 2016. "Frontal Cortex Transcriptome Analysis of Mice Exposed to Electronic Cigarettes During Early Life Stages," IJERPH, MDPI, vol. 13(4), pages 1-14, April.
    3. Teresa N. Brockie & Morgan Heinzelmann & Jessica Gill, 2013. "A Framework to Examine the Role of Epigenetics in Health Disparities among Native Americans," Nursing Research and Practice, Hindawi, vol. 2013, pages 1-9, December.
    4. Hannah Lui Park, 2020. "Epigenetic Biomarkers for Environmental Exposures and Personalized Breast Cancer Prevention," IJERPH, MDPI, vol. 17(4), pages 1-18, February.

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