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Optimization of Biomass Delignification by Extrusion and Analysis of Extrudate Characteristics

Author

Listed:
  • Delon Konan

    (Laboratory of Environmental Biotechnologies, Institut National de la Recherche Scientifique (INRS), Quebec City, QC G1P 4S5, Canada)

  • Adama Ndao

    (Laboratory of Environmental Biotechnologies, Institut National de la Recherche Scientifique (INRS), Quebec City, QC G1P 4S5, Canada)

  • Ekoun Koffi

    (Department of Mechanic and Energy Engineering, Institut National Polytechnique Felix Houphouët Boigny (INPHB), Yamoussoukro BP 1093, Côte d’Ivoire)

  • Saïd Elkoun

    (Center for Innovation in Technological Ecodesign (CITE), University of Sherbrooke, Sherbrooke, QC J1K 2R1, Canada)

  • Mathieu Robert

    (Center for Innovation in Technological Ecodesign (CITE), University of Sherbrooke, Sherbrooke, QC J1K 2R1, Canada)

  • Denis Rodrigue

    (Department of Chemical Engineering, Université Laval, Quebec City, QC G1V 0A6, Canada)

  • Kokou Adjallé

    (Laboratory of Environmental Biotechnologies, Institut National de la Recherche Scientifique (INRS), Quebec City, QC G1P 4S5, Canada)

Abstract

Pretreatment of lignocellulosic biomass remains the primary obstacle to the profitable use of this type of biomass in biorefineries. The challenge lies in the recalcitrance of the lignin-carbohydrate complex to pretreatment, especially the difficulty in removing the lignin to access the carbohydrates (cellulose and hemicellulose). This study had two objectives: (i) to investigate the effect of reactive extrusion on lignocellulosic biomass in terms of delignification percentage and the structural characteristics of the resulting extrudates, and (ii) to propose a novel pretreatment approach involving extrusion technology based on the results of the first objective. Two types of biomasses were used: agricultural residue (corn stover) and forest residue (black spruce chips). By optimizing the extrusion conditions via response surface analysis (RSA), the delignification percentages were significantly improved. For corn stover, the delignification yield increased from 2.3% to 27.4%, while increasing from 1% to 25.3% for black spruce chips. The highest percentages were achieved without the use of sodium hydroxide and for temperatures below 65 °C. Furthermore, the optimized extrudates exhibited important structural changes without any formation of p-cresol, furfural, and 5-hydroxymethylfurfural (HMF) (enzymes and microbial growth-inhibiting compounds). Acetic acid however was detected in corn stover extrudate. The structural changes included the disorganization of the most recalcitrant functional groups, reduction of particle sizes, increase of specific surface areas, and the appearance of microscopic roughness on the particles. Analyzing all the data led to propose a new promising approach to the pretreatment of lignocellulosic biomasses. This approach involves combining extrusion and biodelignification with white rot fungi to improve the enzymatic hydrolysis of carbohydrates.

Suggested Citation

  • Delon Konan & Adama Ndao & Ekoun Koffi & Saïd Elkoun & Mathieu Robert & Denis Rodrigue & Kokou Adjallé, 2025. "Optimization of Biomass Delignification by Extrusion and Analysis of Extrudate Characteristics," Waste, MDPI, vol. 3(2), pages 1-27, March.
    • Handle: RePEc:gam:jwaste:v:3:y:2025:i:2:p:12-:d:1619651
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    References listed on IDEAS

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    1. Akobi, Chinaza & Yeo, Hyeongu & Hafez, Hisham & Nakhla, George, 2016. "Single-stage and two-stage anaerobic digestion of extruded lignocellulosic biomass," Applied Energy, Elsevier, vol. 184(C), pages 548-559.
    2. Wang, Min & Zhao, Jinhua, 2018. "Are renewable energy policies climate friendly? The role of capacity constraints and market power," Journal of Environmental Economics and Management, Elsevier, vol. 90(C), pages 41-60.
    3. Ong, Victor Zhenquan & Wu, Ta Yeong, 2020. "An application of ultrasonication in lignocellulosic biomass valorisation into bio-energy and bio-based products," Renewable and Sustainable Energy Reviews, Elsevier, vol. 132(C).
    4. Duque, Aleta & Manzanares, Paloma & Ballesteros, Mercedes, 2017. "Extrusion as a pretreatment for lignocellulosic biomass: Fundamentals and applications," Renewable Energy, Elsevier, vol. 114(PB), pages 1427-1441.
    5. William Herbert Lee Stafford & George Adrian Lotter & Graham Paul von Maltitz & Alan Colin Brent, 2019. "Biofuels technology development in Southern Africa," Development Southern Africa, Taylor & Francis Journals, vol. 36(2), pages 155-174, March.
    6. Román-Ramírez, L.A. & Marco, J., 2022. "Design of experiments applied to lithium-ion batteries: A literature review," Applied Energy, Elsevier, vol. 320(C).
    7. Long, Jinxing & Xu, Ying & Wang, Tiejun & Yuan, Zhengqiu & Shu, Riyang & Zhang, Qi & Ma, Longlong, 2015. "Efficient base-catalyzed decomposition and in situ hydrogenolysis process for lignin depolymerization and char elimination," Applied Energy, Elsevier, vol. 141(C), pages 70-79.
    8. Audibert, Edwige & Floret, Juliette & Quintero, Adriana & Martel, Frédéric & Rémond, Caroline & Paës, Gabriel, 2025. "Determination of trade-offs between 2G bioethanol production yields and pretreatment costs for industrially steam exploded woody biomass," Applied Energy, Elsevier, vol. 380(C).
    9. Ashfaq Ahmed & Muhammad S. Abu Bakar & Abdul Razzaq & Syarif Hidayat & Farrukh Jamil & Muhammad Nadeem Amin & Rahayu S. Sukri & Noor S. Shah & Young-Kwon Park, 2021. "Characterization and Thermal Behavior Study of Biomass from Invasive Acacia mangium Species in Brunei Preceding Thermochemical Conversion," Sustainability, MDPI, vol. 13(9), pages 1-13, May.
    10. Woochul Jung & Dhanalekshmi Savithri & Ratna Sharma-Shivappa & Praveen Kolar, 2018. "Changes in Lignin Chemistry of Switchgrass due to Delignification by Sodium Hydroxide Pretreatment," Energies, MDPI, vol. 11(2), pages 1-12, February.
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