IDEAS home Printed from https://ideas.repec.org/a/gam/jeners/v7y2014i5p3383-3399d36323.html
   My bibliography  Save this article

A Biofuel Similar to Biodiesel Obtained by Using a Lipase from Rhizopus oryzae , Optimized by Response Surface Methodology

Author

Listed:
  • Carlos Luna

    (Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie,14014 Córdoba, Spain)

  • Cristobal Verdugo

    (Laboratorio de Estudios Cristalográficos, IACT, CSIC-University of Granada, Avenida de las Palmeras 4, 18100 Armilla, Granada, Spain)

  • Enrique D. Sancho

    (Department of Microbiology, University of Córdoba, Campus de Rabanales, Ed. Severo Ochoa, 14014 Córdoba, Spain)

  • Diego Luna

    (Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie,14014 Córdoba, Spain
    Seneca Green Catalyst S.L., Campus de Rabanales, 14014 Córdoba, Spain)

  • Juan Calero

    (Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie,14014 Córdoba, Spain)

  • Alejandro Posadillo

    (Seneca Green Catalyst S.L., Campus de Rabanales, 14014 Córdoba, Spain)

  • Felipa M. Bautista

    (Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie,14014 Córdoba, Spain)

  • Antonio A. Romero

    (Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie,14014 Córdoba, Spain)

Abstract

A new biodiesel-like biofuel is obtained by the enzymatic ethanolysis reaction of sunflower oil with ethanol, in free solvent media, by using BIOLIPASE-R, a multipurpose alimentary additive from Biocon ® -Spain that is a low cost lipase from a strain of Rhizopus oryzae . This biofuel is composed by two parts of fatty acid ethyl esters (FAEE) and one of monoglyceride (MG), which in this form integrates glycerol, through the application of the 1,3-selective lipases. Thus, this process minimizes waste generation and maximizes the efficiency of the process because no residual glycerol is produced. Response surface methodology (RSM) is employed to evaluate the main reaction parameters (reaction temperature, oil/ethanol ratio and pH) on the sunflower oil conversion. Water content and amount of lipase were also previously investigated. Regarding the results, we found that it operates optimally with a water content of the reaction medium of 0.15%, 0.05%–0.1% lipase by weight relative to the weight of oil used, 20 °C, volume ratio (mL/mL) oil/ethanol 12/3.5 and pH 12 (by addition of 50 µL of 10 N NaOH solution). These results have proven a very good efficiency of the biocatalyst in the studied selective process.

Suggested Citation

  • Carlos Luna & Cristobal Verdugo & Enrique D. Sancho & Diego Luna & Juan Calero & Alejandro Posadillo & Felipa M. Bautista & Antonio A. Romero, 2014. "A Biofuel Similar to Biodiesel Obtained by Using a Lipase from Rhizopus oryzae , Optimized by Response Surface Methodology," Energies, MDPI, vol. 7(5), pages 1-17, May.
    • Handle: RePEc:gam:jeners:v:7:y:2014:i:5:p:3383-3399:d:36323
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1996-1073/7/5/3383/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1996-1073/7/5/3383/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Haseeb, A.S.M.A. & Sia, S.Y. & Fazal, M.A. & Masjuki, H.H., 2010. "Effect of temperature on tribological properties of palm biodiesel," Energy, Elsevier, vol. 35(3), pages 1460-1464.
    2. Xu, Yufu & Wang, Qiongjie & Hu, Xianguo & Li, Chuan & Zhu, Xifeng, 2010. "Characterization of the lubricity of bio-oil/diesel fuel blends by high frequency reciprocating test rig," Energy, Elsevier, vol. 35(1), pages 283-287.
    3. Demirbas, Ayhan, 2009. "Political, economic and environmental impacts of biofuels: A review," Applied Energy, Elsevier, vol. 86(Supplemen), pages 108-117, November.
    4. Oh, Pin Pin & Lau, Harrison Lik Nang & Chen, Junghui & Chong, Mei Fong & Choo, Yuen May, 2012. "A review on conventional technologies and emerging process intensification (PI) methods for biodiesel production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 16(7), pages 5131-5145.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Juan Calero & Diego Luna & Carlos Luna & Felipa M. Bautista & Beatriz Hurtado & Antonio A. Romero & Alejandro Posadillo & Rafael Estevez, 2019. "Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology," Energies, MDPI, vol. 12(5), pages 1-15, March.
    2. Rafael Estevez & Laura Aguado-Deblas & Francisco J. López-Tenllado & Carlos Luna & Juan Calero & Antonio A. Romero & Felipa M. Bautista & Diego Luna, 2022. "Biodiesel Is Dead: Long Life to Advanced Biofuels—A Comprehensive Critical Review," Energies, MDPI, vol. 15(9), pages 1-39, April.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Calero, Juan & Luna, Diego & Sancho, Enrique D. & Luna, Carlos & Bautista, Felipa M. & Romero, Antonio A. & Posadillo, Alejandro & Berbel, Julio & Verdugo-Escamilla, Cristóbal, 2015. "An overview on glycerol-free processes for the production of renewable liquid biofuels, applicable in diesel engines," Renewable and Sustainable Energy Reviews, Elsevier, vol. 42(C), pages 1437-1452.
    2. Carlos Luna & Enrique Sancho & Diego Luna & Verónica Caballero & Juan Calero & Alejandro Posadillo & Cristóbal Verdugo & Felipa M. Bautista & Antonio A. Romero, 2013. "Biofuel that Keeps Glycerol as Monoglyceride by 1,3-Selective Ethanolysis with Pig Pancreatic Lipase Covalently Immobilized on AlPO 4 Support," Energies, MDPI, vol. 6(8), pages 1-22, July.
    3. Jakeria, M.R. & Fazal, M.A. & Haseeb, A.S.M.A., 2014. "Influence of different factors on the stability of biodiesel: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 30(C), pages 154-163.
    4. Maru, Marcia M. & Almeida, Clara M. & Silva, Rui F. & Achete, Carlos A., 2013. "Assessment of boundary lubrication in biodiesels by nanotribological tests," Energy, Elsevier, vol. 55(C), pages 273-277.
    5. Maru, Marcia M. & Trommer, Rafael M. & Cavalcanti, Kátia F. & Figueiredo, Elizabeth S. & Silva, Rui F. & Achete, Carlos A., 2014. "The Stribeck curve as a suitable characterization method of the lubricity of biodiesel and diesel blends," Energy, Elsevier, vol. 69(C), pages 673-681.
    6. Li, Wen-Chao & Li, Xia & Zhu, Jia-Qing & Qin, Lei & Li, Bing-Zhi & Yuan, Ying-Jin, 2018. "Improving xylose utilization and ethanol production from dry dilute acid pretreated corn stover by two-step and fed-batch fermentation," Energy, Elsevier, vol. 157(C), pages 877-885.
    7. Ba, Birome Holo & Prins, Christian & Prodhon, Caroline, 2016. "Models for optimization and performance evaluation of biomass supply chains: An Operations Research perspective," Renewable Energy, Elsevier, vol. 87(P2), pages 977-989.
    8. Lechón, Y. & de la Rúa, C. & Rodríguez, I. & Caldés, N., 2019. "Socioeconomic implications of biofuels deployment through an Input-Output approach. A case study in Uruguay," Renewable and Sustainable Energy Reviews, Elsevier, vol. 104(C), pages 178-191.
    9. Baudry, Gino & Delrue, Florian & Legrand, Jack & Pruvost, Jérémy & Vallée, Thomas, 2017. "The challenge of measuring biofuel sustainability: A stakeholder-driven approach applied to the French case," Renewable and Sustainable Energy Reviews, Elsevier, vol. 69(C), pages 933-947.
    10. Pöschl, Martina & Ward, Shane & Owende, Philip, 2010. "Evaluation of energy efficiency of various biogas production and utilization pathways," Applied Energy, Elsevier, vol. 87(11), pages 3305-3321, November.
    11. Haseeb, A.S.M.A. & Jun, T.S. & Fazal, M.A. & Masjuki, H.H., 2011. "Degradation of physical properties of different elastomers upon exposure to palm biodiesel," Energy, Elsevier, vol. 36(3), pages 1814-1819.
    12. Winden, Matthew & Cruze, Nathan & Haab, Tim & Bakshi, Bhavik, 2015. "Monetized value of the environmental, health and resource externalities of soy biodiesel," Energy Economics, Elsevier, vol. 47(C), pages 18-24.
    13. Solmaz, Hamit & Ardebili, Seyed Mohammad Safieddin & Calam, Alper & Yılmaz, Emre & İpci, Duygu, 2021. "Prediction of performance and exhaust emissions of a CI engine fueled with multi-wall carbon nanotube doped biodiesel-diesel blends using response surface method," Energy, Elsevier, vol. 227(C).
    14. Phanankosi Moyo & Mahluli Moyo & Donatus Dube & Oswell Rusinga, 2013. "Biofuel Policy as a Key Driver for Sustainable Development in the Biofuel Sector: The Missing Ingredient in Zimbabwe’s Biofuel Pursuit," Modern Applied Science, Canadian Center of Science and Education, vol. 8(1), pages 1-36, February.
    15. Wang, Kai-Hua & Su, Chi-Wei & Lobonţ, Oana-Ramona & Umar, Muhammad, 2021. "Whether crude oil dependence and CO2 emissions influence military expenditure in net oil importing countries?," Energy Policy, Elsevier, vol. 153(C).
    16. Tan, Raymond R. & Aviso, Kathleen B. & Barilea, Ivan U. & Culaba, Alvin B. & Cruz, Jose B., 2012. "A fuzzy multi-regional input–output optimization model for biomass production and trade under resource and footprint constraints," Applied Energy, Elsevier, vol. 90(1), pages 154-160.
    17. Haseeb, A.S.M.A. & Masjuki, H.H. & Siang, C.T. & Fazal, M.A., 2010. "Compatibility of elastomers in palm biodiesel," Renewable Energy, Elsevier, vol. 35(10), pages 2356-2361.
    18. Sacchelli, Sandro & De Meo, Isabella & Paletto, Alessandro, 2013. "Bioenergy production and forest multifunctionality: A trade-off analysis using multiscale GIS model in a case study in Italy," Applied Energy, Elsevier, vol. 104(C), pages 10-20.
    19. Bilgili, Faik & Koçak, Emrah & Bulut, Ümit & Kuşkaya, Sevda, 2017. "Can biomass energy be an efficient policy tool for sustainable development?," Renewable and Sustainable Energy Reviews, Elsevier, vol. 71(C), pages 830-845.
    20. Neves, Renato Cruz & Klein, Bruno Colling & da Silva, Ricardo Justino & Rezende, Mylene Cristina Alves Ferreira & Funke, Axel & Olivarez-Gómez, Edgardo & Bonomi, Antonio & Maciel-Filho, Rubens, 2020. "A vision on biomass-to-liquids (BTL) thermochemical routes in integrated sugarcane biorefineries for biojet fuel production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 119(C).

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jeners:v:7:y:2014:i:5:p:3383-3399:d:36323. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.