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Isolation and Fatty Acid Profile of Selected Microalgae Strains from the Red Sea for Biofuel Production

Author

Listed:
  • Hugo Pereira

    (Centre of Marine Sciences, University of Algarve, Faro 8005-139, Portugal)

  • Luísa Barreira

    (Centre of Marine Sciences, University of Algarve, Faro 8005-139, Portugal)

  • Luísa Custódio

    (Centre of Marine Sciences, University of Algarve, Faro 8005-139, Portugal)

  • Salman Alrokayan

    (College of Science, King Saud University, Riyadh 11451, Saudi Arabia)

  • Fouzi Mouffouk

    (National Guard Health Affairs (NGHA), King Abdullah International Medical Research Centre (KAIMRC), Jeddah 21423, Saudi Arabia
    Chemistry Department, Faculty of Science, Kuwait University, Safat 13060, Kuwait)

  • João Varela

    (Centre of Marine Sciences, University of Algarve, Faro 8005-139, Portugal)

  • Khalid M. Abu-Salah

    (King Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11451, Saudi Arabia)

  • Radhouan Ben-Hamadou

    (Centre of Marine Sciences, University of Algarve, Faro 8005-139, Portugal
    Department of Biological and Environmental Sciences, College of Arts and Sciences, Qatar University, Doha, Qatar)

Abstract

The isolation of lipid-rich autochthonous strains of microalgae is a crucial stage for the development of a microalgae-based biofuel production plant, as these microalgae already have the necessary adaptations to withstand competition, predation and the temperatures observed at each production site. This is particularly important in extreme climates such as in Saudi Arabia. Resorting to fluorescence activated cell sorting (FACS) we screened for and isolated several microalgal strains from samples collected from the Red Sea. Relying on the fluorescence of BODIPY 505/515 (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diazasindacene) and growth performance, four promising candidates were identified and the total lipid content and fatty acid profile was assessed for biofuels production. Selected isolates were classified as chlorophytes, belonging to three different genera: Picochlorum , Nannochloris and Desmochloris . The lipid contents were assessed microscopically by means of BODIPY 505/515-associated fluorescence to detect intracellular lipid bodies, which revealed several lipid drops in all selected strains. This result was confirmed by lipid gravimetric determination, which demonstrated that all strains under study presented inner cell lipid contents ranging from 20% to 25% of the biomass dry weight. Furthermore, the fatty acid methyl esters profile of all strains seems ideal for biodiesel production due to a low degree of polyunsaturated fatty acid methyl esters and high amount of palmitic and oleic acids.

Suggested Citation

  • Hugo Pereira & Luísa Barreira & Luísa Custódio & Salman Alrokayan & Fouzi Mouffouk & João Varela & Khalid M. Abu-Salah & Radhouan Ben-Hamadou, 2013. "Isolation and Fatty Acid Profile of Selected Microalgae Strains from the Red Sea for Biofuel Production," Energies, MDPI, vol. 6(6), pages 1-11, May.
    • Handle: RePEc:gam:jeners:v:6:y:2013:i:6:p:2773-2783:d:26129
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    References listed on IDEAS

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    1. Rahman, Syed Masiur & Khondaker, A.N., 2012. "Mitigation measures to reduce greenhouse gas emissions and enhance carbon capture and storage in Saudi Arabia," Renewable and Sustainable Energy Reviews, Elsevier, vol. 16(5), pages 2446-2460.
    2. Mata, Teresa M. & Martins, António A. & Caetano, Nidia. S., 2010. "Microalgae for biodiesel production and other applications: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 14(1), pages 217-232, January.
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    1. Rajabi Islami, Houman & Assareh, Reza, 2020. "Enhancement effects of ferric ion concentrations on growth and lipid characteristics of freshwater microalga Chlorococcum oleofaciens KF584224.1 for biodiesel production," Renewable Energy, Elsevier, vol. 149(C), pages 264-272.
    2. Touria Bounnit & Imen Saadaoui & Rihab Rasheed & Kira Schipper & Maryam Al Muraikhi & Hareb Al Jabri, 2020. "Sustainable Production of Nannochloris atomus Biomass Towards Biodiesel Production," Sustainability, MDPI, vol. 12(5), pages 1-21, March.
    3. J. Jed Brown & Probir Das & Mohammad Al-Saidi, 2018. "Sustainable Agriculture in the Arabian/Persian Gulf Region Utilizing Marginal Water Resources: Making the Best of a Bad Situation," Sustainability, MDPI, vol. 10(5), pages 1-16, April.
    4. Ramganesh Selvarajan & Tamás Felföldi & Tamás Tauber & Elumalai Sanniyasi & Timothy Sibanda & Memory Tekere, 2015. "Screening and Evaluation of Some Green Algal Strains (Chlorophyceae) Isolated from Freshwater and Soda Lakes for Biofuel Production," Energies, MDPI, vol. 8(7), pages 1-20, July.
    5. Dasgupta, Chitralekha Nag & Suseela, M.R. & Mandotra, S.K. & Kumar, Pankaj & Pandey, Manish K. & Toppo, Kiran & Lone, J.A., 2015. "Dual uses of microalgal biomass: An integrative approach for biohydrogen and biodiesel production," Applied Energy, Elsevier, vol. 146(C), pages 202-208.

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