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Bioactive Compounds, Ruminal Fermentation, and Anthelmintic Activity of Specialty Coffee and Spent Coffee Grounds In Vitro

Author

Listed:
  • Matej Leško

    (Institute of Animal Physiology, Centre of Biosciences of Slovak Academy of Sciences, Šoltésovej 4-6, 040 01 Košice, Slovakia)

  • Daniel Petrič

    (Institute of Animal Physiology, Centre of Biosciences of Slovak Academy of Sciences, Šoltésovej 4-6, 040 01 Košice, Slovakia)

  • Matúš Várady

    (Department of Food Hygiene, Technology and Safety, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovakia)

  • Pola Sidoruk

    (Department of Animal Nutrition, Poznan University of Life Sciences, Wolynska 33, 60-637 Poznan, Poland)

  • Robert Mikula

    (Department of Animal Nutrition, Poznan University of Life Sciences, Wolynska 33, 60-637 Poznan, Poland)

  • Sylwester Ślusarczyk

    (Department of Pharmaceutical Biology and Botany, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland)

  • Paweł Edward Hodurek

    (Department of Pharmaceutical Biology and Botany, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland)

  • Michaela Komáromyová

    (Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01 Košice, Slovakia)

  • Michal Babják

    (Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01 Košice, Slovakia)

  • Marián Várady

    (Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01 Košice, Slovakia)

  • Amlan Kumar Patra

    (American Institute for Goat Research, Langston University, 100 Success Ave, Langston, OK 73050, USA)

  • Adam Cieslak

    (Department of Animal Nutrition, Poznan University of Life Sciences, Wolynska 33, 60-637 Poznan, Poland)

  • Zora Váradyová

    (Institute of Animal Physiology, Centre of Biosciences of Slovak Academy of Sciences, Šoltésovej 4-6, 040 01 Košice, Slovakia)

Abstract

We quantified the bioactive compounds of Ethiopian coffee (ETH), spent coffee grounds SCGs from ETH (SCG-ETH), and mixed SCGs (SCG-MIX) prepared by filtration methods and investigated the effect of SCG-ETH on ruminal fermentation as well as the anthelmintic activity of ETH. Three substrates, meadow hay (MH)-barley grain (MH-BG), MH-SCG-ETH, and BG-SCG-ETH (1:1 w / w ), were fermented using an in vitro gas production technique. The bioactive compounds were quantitatively analyzed using ultra-high-resolution mass spectrometry. We performed an in vitro larval development test to determine the anthelmintic effect of an aqueous extract of ETH against the gastrointestinal nematode (GIN) Haemonchus contortus . The total content of bioactive compounds was highest in SCG-ETH, followed by SCG-MIX and ETH (35.2, 31.2, and 20.9 mg/g dry matter, respectively). Total gas and methane production ( p < 0.001) were decreased by both MH-SCG-ETH and BG-SCG-ETH. The in vitro digestibility of dry matter was higher for MH-SCG-ETH and BG-SCG-ETH than for MH-BG. The aqueous ETH extract exhibited a strong larvicidal effect, with a mean lethal dose of 13.2 mg/mL for 50% mortality and 31.9 mg/L for 99% mortality. SCG substrates have the potential to modulate ruminal fermentation and serve as a source of anthelmintic bioactive compounds against GINs in ruminants.

Suggested Citation

  • Matej Leško & Daniel Petrič & Matúš Várady & Pola Sidoruk & Robert Mikula & Sylwester Ślusarczyk & Paweł Edward Hodurek & Michaela Komáromyová & Michal Babják & Marián Várady & Amlan Kumar Patra & Ada, 2025. "Bioactive Compounds, Ruminal Fermentation, and Anthelmintic Activity of Specialty Coffee and Spent Coffee Grounds In Vitro," Agriculture, MDPI, vol. 15(14), pages 1-15, July.
    • Handle: RePEc:gam:jagris:v:15:y:2025:i:14:p:1515-:d:1701061
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    References listed on IDEAS

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    1. Daniel Petrič & Michaela Komáromyová & Dominika Batťányi & Martyna Kozłowska & Weronika Filipiak & Anna Łukomska & Sylwester Ślusarczyk & Malgorzata Szumacher-Strabel & Adam Cieślak & Marián Várady & , 2022. "Effect of Sainfoin ( Onobrychis viciifolia ) Pellets on Rumen Microbiome and Histopathology in Lambs Exposed to Gastrointestinal Nematodes," Agriculture, MDPI, vol. 12(2), pages 1-14, February.
    2. Yen Pham & Kathryn Reardon-Smith & Shahbaz Mushtaq & Geoff Cockfield, 2019. "The impact of climate change and variability on coffee production: a systematic review," Climatic Change, Springer, vol. 156(4), pages 609-630, October.
    3. Klára Mikulová & Daniel Petrič & Michaela Komáromyová & Dominika Batťányi & Martyna Kozłowska & Adam Cieslak & Sylwester Ślusarczyk & Marián Várady & Zora Váradyová, 2023. "Growth Performance and Ruminal Fermentation in Lambs with Endoparasites and In Vitro Effect of Medicinal Plants," Agriculture, MDPI, vol. 13(9), pages 1-12, September.
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