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Two-step pH regulating ethanol production through continuous CO/CO2 gas fermentation by mixed bacteria from rabbit faeces

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  • Guo, Xiangjun
  • Huang, Yun
  • Tang, Yunheng
  • Xia, Ao
  • Zhu, Xianqing
  • Zhu, Xun
  • Liao, Qiang

Abstract

Mixed bacterial syngas fermentation is a promising carbon-neutral method for producing acetic acid and ethanol. However, the bacterial community and metabolic pathways were different in the growth and ethanol production stages, whereas the activity of the bacterial community greatly depended on the pH of the culture. In this study, the effect of pH on syngas fermentation performance and microbial community composition under continuous gas supply was investigated. The mixed bacteria grew fastest at a pH of 7, while a constant pH of 6 led to the highest ethanol concentration of 5.15 g L−1, which was 160 % higher than that at pH 7. Based on this, a two-step pH regulation method was proposed in this study to improve the ethanol production. Initially, the culture pH was maintained at 7 to create optimal growth conditions. Once bacterial density stabilized, it was then changed to a pH 6 to promote ethanol production. By doing this, compared with the control group, the maximum ethanol concentration (5.85 g L−1) increased by 148.9 %, the ethanol production rate (2.41 g L−1 d−1) increased by more than 8 times. This two-step pH regulation method ensured a high bacterial density and effectively stimulated ethanol production during syngas fermentation.

Suggested Citation

  • Guo, Xiangjun & Huang, Yun & Tang, Yunheng & Xia, Ao & Zhu, Xianqing & Zhu, Xun & Liao, Qiang, 2024. "Two-step pH regulating ethanol production through continuous CO/CO2 gas fermentation by mixed bacteria from rabbit faeces," Renewable Energy, Elsevier, vol. 228(C).
    • Handle: RePEc:eee:renene:v:228:y:2024:i:c:s0960148124006323
    DOI: 10.1016/j.renene.2024.120564
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    References listed on IDEAS

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    1. Hanno Richter & Michael E. Martin & Largus T. Angenent, 2013. "A Two-Stage Continuous Fermentation System for Conversion of Syngas into Ethanol," Energies, MDPI, vol. 6(8), pages 1-14, August.
    2. Lin, Boqiang & Li, Zheng, 2022. "Towards world's low carbon development: The role of clean energy," Applied Energy, Elsevier, vol. 307(C).
    3. Tang, Yunheng & Huang, Yun & Gan, Wentian & Xia, Ao & Liao, Qiang & Zhu, Xianqing, 2021. "Ethanol production from gas fermentation: Rapid enrichment and domestication of bacterial community with continuous CO/CO2 gas," Renewable Energy, Elsevier, vol. 175(C), pages 337-344.
    4. Wang, Chen & Raza, Syed Ali & Adebayo, Tomiwa Sunday & Yi, Sun & Shah, Muhammad Ibrahim, 2023. "The roles of hydro, nuclear and biomass energy towards carbon neutrality target in China: A policy-based analysis," Energy, Elsevier, vol. 262(PA).
    5. Maghzian, Ali & Aslani, Alireza & Zahedi, Rahim & Yaghoubi, Milad, 2023. "How to effectively produce value-added products from microalgae?," Renewable Energy, Elsevier, vol. 204(C), pages 262-276.
    6. Shen, Yanwen & Brown, Robert C. & Wen, Zhiyou, 2017. "Syngas fermentation by Clostridium carboxidivorans P7 in a horizontal rotating packed bed biofilm reactor with enhanced ethanol production," Applied Energy, Elsevier, vol. 187(C), pages 585-594.
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