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High gravity enzymatic hydrolysis of hydrothermal and ultrasonic pretreated big bluestem with recycling prehydrolysate water

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  • Xu, Youjie
  • Zhang, Ke
  • Wang, Donghai

Abstract

Enhancing sugar concentration and minimizing water consumption are key objectives for future cellulosic biofuel economics. To achieve those objectives, high-solids loading pretreatment and enzymatic hydrolysis (up to 20%, w/v) were studied. Big bluestem was selected and combined hydrothermal and ultrasonic treatment without chemicals addition was carried out in this study. Optimal high-solids loading pretreatment (16%, w/v) was identified in the ultrasonic reactor at 200 °C for 30 min. High-solids enzymatic hydrolysis (12%, w/v) was inefficient in the laboratory rotary shaker. However, using a horizontal reactor with good mixing is effective for high solids loading (20%, w/v), yielding 75 g/L of glucose. Minimum water to detoxify pretreated biomass while maintaining high sugar yields was 10 mL/g, which reduced by 50% as compared to the conventional washing process for steam-treated wheat straw. Recycling pretreatment liquor to treat the next batch of biomass was proved to be feasible without affecting the sugar yields.

Suggested Citation

  • Xu, Youjie & Zhang, Ke & Wang, Donghai, 2017. "High gravity enzymatic hydrolysis of hydrothermal and ultrasonic pretreated big bluestem with recycling prehydrolysate water," Renewable Energy, Elsevier, vol. 114(PB), pages 351-356.
    • Handle: RePEc:eee:renene:v:114:y:2017:i:pb:p:351-356
    DOI: 10.1016/j.renene.2017.07.045
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    References listed on IDEAS

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    1. Zhang, Ke & Johnson, Loretta & Prasad, P.V. Vara & Pei, Zhijian & Yuan, Wenqiao & Wang, Donghai, 2015. "Comparison of big bluestem with other native grasses: Chemical composition and biofuel yield," Energy, Elsevier, vol. 83(C), pages 358-365.
    2. Zhang, Ke & Johnson, Loretta & Vara Prasad, P.V. & Pei, Zhijian & Wang, Donghai, 2015. "Big bluestem as a bioenergy crop: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 52(C), pages 740-756.
    3. Kim, Tae Hoon & Kim, Tae Hyun, 2014. "Overview of technical barriers and implementation of cellulosic ethanol in the U.S," Energy, Elsevier, vol. 66(C), pages 13-19.
    4. Ruiz, Héctor A. & Rodríguez-Jasso, Rosa M. & Fernandes, Bruno D. & Vicente, António A. & Teixeira, José A., 2013. "Hydrothermal processing, as an alternative for upgrading agriculture residues and marine biomass according to the biorefinery concept: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 21(C), pages 35-51.
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    1. Chen, Jiaxin & Zhang, Biying & Luo, Lingli & Zhang, Fan & Yi, Yanglei & Shan, Yuanyuan & Liu, Bianfang & Zhou, Yuan & Wang, Xin & Lü, Xin, 2021. "A review on recycling techniques for bioethanol production from lignocellulosic biomass," Renewable and Sustainable Energy Reviews, Elsevier, vol. 149(C).
    2. Pontes, Rita & Romaní, Aloia & Michelin, Michele & Domingues, Lucília & Teixeira, José & Nunes, João, 2018. "Comparative autohydrolysis study of two mixtures of forest and marginal land resources for co-production of biofuels and value-added compounds," Renewable Energy, Elsevier, vol. 128(PA), pages 20-29.

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