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The De-Ubiquitinylating Enzyme, USP2, Is Associated with the Circadian Clockwork and Regulates Its Sensitivity to Light

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  • Heather Dehlin Scoma
  • Monica Humby
  • Geetha Yadav
  • Qingjiong Zhang
  • Joseph Fogerty
  • Joseph C Besharse

Abstract

We have identified a novel component of the circadian clock that regulates its sensitivity to light at the evening light to dark transition. USP2 (Ubiquitin Specific Protease 2), which de-ubiquitinylates and stabilizes target proteins, is rhythmically expressed in multiple tissues including the SCN. We have developed a knockout model of USP2 and found that exposure to low irradiance light at ZT12 increases phase delays of USP2−/− mice compared to wildtype. We additionally show that USP2b is in a complex with several clock components and regulates the stability and turnover of BMAL1, which in turn alters the expression of several CLOCK/BMAL1 controlled genes. Rhythmic expression of USP2 in the SCN and other tissues offers a new level of control of the clock machinery through de-ubiqutinylation and suggests a role for USP2 during circadian adaptation to environmental day length changes.

Suggested Citation

  • Heather Dehlin Scoma & Monica Humby & Geetha Yadav & Qingjiong Zhang & Joseph Fogerty & Joseph C Besharse, 2011. "The De-Ubiquitinylating Enzyme, USP2, Is Associated with the Circadian Clockwork and Regulates Its Sensitivity to Light," PLOS ONE, Public Library of Science, vol. 6(9), pages 1-14, September.
    • Handle: RePEc:plo:pone00:0025382
    DOI: 10.1371/journal.pone.0025382
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