IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0076391.html
   My bibliography  Save this article

Comprehensive Mapping of Regional Expression of the Clock Protein PERIOD2 in Rat Forebrain across the 24-h Day

Author

Listed:
  • Valerie L Harbour
  • Yuval Weigl
  • Barry Robinson
  • Shimon Amir

Abstract

In mammals, a light-entrainable clock located in the suprachiasmatic nucleus (SCN) regulates circadian rhythms by synchronizing oscillators throughout the brain and body. Notably, the nature of the relation between the SCN clock and subordinate oscillators in the rest of the brain is not well defined. We performed a high temporal resolution analysis of the expression of the circadian clock protein PERIOD2 (PER2) in the rat forebrain to characterize the distribution, amplitude and phase of PER2 rhythms across different regions. Eighty-four LEW/Crl male rats were entrained to a 12-h: 12-h light/dark cycle, and subsequently perfused every 30 min across the 24-h day for a total of 48 time-points. PER2 expression was assessed with immunohistochemistry and analyzed using automated cell counts. We report the presence of PER2 expression in 20 forebrain areas important for a wide range of motivated and appetitive behaviors including the SCN, bed nucleus, and several regions of the amygdala, hippocampus, striatum, and cortex. Eighteen areas displayed significant PER2 rhythms, which peaked at different times of day. Our data demonstrate a previously uncharacterized regional distribution of rhythms of a clock protein expression in the brain that provides a sound basis for future studies of circadian clock function in animal models of disease.

Suggested Citation

  • Valerie L Harbour & Yuval Weigl & Barry Robinson & Shimon Amir, 2013. "Comprehensive Mapping of Regional Expression of the Clock Protein PERIOD2 in Rat Forebrain across the 24-h Day," PLOS ONE, Public Library of Science, vol. 8(10), pages 1-14, October.
    • Handle: RePEc:plo:pone00:0076391
    DOI: 10.1371/journal.pone.0076391
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0076391
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0076391&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0076391?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Binhai Zheng & David W. Larkin & Urs Albrecht & Zhong Sheng Sun & Marijke Sage & Gregor Eichele & Cheng Chi Lee & Allan Bradley, 1999. "The mPer2 gene encodes a functional component of the mammalian circadian clock," Nature, Nature, vol. 400(6740), pages 169-173, July.
    2. Steven M. Reppert & David R. Weaver, 2002. "Coordination of circadian timing in mammals," Nature, Nature, vol. 418(6901), pages 935-941, August.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Sherin Al-Safadi & Aya Al-Safadi & Marie Branchaud & Spencer Rutherford & Arun Dayanandan & Barry Robinson & Shimon Amir, 2014. "Stress-Induced Changes in the Expression of the Clock Protein PERIOD1 in the Rat Limbic Forebrain and Hypothalamus: Role of Stress Type, Time of Day, and Predictability," PLOS ONE, Public Library of Science, vol. 9(10), pages 1-16, October.
    2. Valerie L Harbour & Yuval Weigl & Barry Robinson & Shimon Amir, 2014. "Phase Differences in Expression of Circadian Clock Genes in the Central Nucleus of the Amygdala, Dentate Gyrus, and Suprachiasmatic Nucleus in the Rat," PLOS ONE, Public Library of Science, vol. 9(7), pages 1-9, July.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Toru Nakamura & Toru Takumi & Atsuko Takano & Naoko Aoyagi & Kazuhiro Yoshiuchi & Zbigniew R Struzik & Yoshiharu Yamamoto, 2008. "Of Mice and Men — Universality and Breakdown of Behavioral Organization," PLOS ONE, Public Library of Science, vol. 3(4), pages 1-8, April.
    2. Francesco Riganello & Valeria Prada & Andres Soddu & Carol di Perri & Walter G. Sannita, 2019. "Circadian Rhythms and Measures of CNS/Autonomic Interaction," IJERPH, MDPI, vol. 16(13), pages 1-11, July.
    3. Purificación Gómez-Abellán & Antoni Díez-Noguera & Juan A Madrid & Juan A Luján & José M Ordovás & Marta Garaulet, 2012. "Glucocorticoids Affect 24 h Clock Genes Expression in Human Adipose Tissue Explant Cultures," PLOS ONE, Public Library of Science, vol. 7(12), pages 1-10, December.
    4. Nguyen, Ha Trong & Zubrick, Stephen R. & Mitrou, Francis, 2022. "The effects of sleep duration on child health and development," GLO Discussion Paper Series 1150, Global Labor Organization (GLO).
    5. Qiang Bao & Di Liu & Yujiao Guo & Wang Gu & Zhengfeng Cao & Yu Zhang & Yang Zhang & Qi Xu & Guohong Chen, 2023. "Melatonin Secretion in Regulating the Circadian Rhythms of Reproduction in Goose ( Anser cygnoides )," Agriculture, MDPI, vol. 13(8), pages 1-16, August.
    6. Toru Nakamura & Toru Takumi & Atsuko Takano & Fumiyuki Hatanaka & Yoshiharu Yamamoto, 2013. "Characterization and Modeling of Intermittent Locomotor Dynamics in Clock Gene-Deficient Mice," PLOS ONE, Public Library of Science, vol. 8(3), pages 1-8, March.
    7. Francisco J Sánchez Muniz & Cristina Simón Martín, 2017. "Clock Genes, Chronodisruption, Nutrition and Obesity," Current Research in Diabetes & Obesity Journal, Juniper Publishers Inc., vol. 3(2), pages 1-62:3, July.
    8. Henson, Michael A., 2013. "Multicellular models of intercellular synchronization in circadian neural networks," Chaos, Solitons & Fractals, Elsevier, vol. 50(C), pages 48-64.
    9. Andrew E. Warfield & Pooja Gupta & Madison M. Ruhmann & Quiana L. Jeffs & Genevieve C. Guidone & Hannah W. Rhymes & McKenzi I. Thompson & William D. Todd, 2023. "A brainstem to circadian system circuit links Tau pathology to sundowning-related disturbances in an Alzheimer’s disease mouse model," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    10. Cheng-Kang Chiang & Neel Mehta & Abhilasha Patel & Peng Zhang & Zhibin Ning & Janice Mayne & Warren Y L Sun & Hai-Ying M Cheng & Daniel Figeys, 2014. "The Proteomic Landscape of the Suprachiasmatic Nucleus Clock Reveals Large-Scale Coordination of Key Biological Processes," PLOS Genetics, Public Library of Science, vol. 10(10), pages 1-15, October.
    11. Li, Ying & Liu, Zengrong, 2015. "Dynamical mechanism of Bmal1/Rev-erbα loop in circadian clock," Physica A: Statistical Mechanics and its Applications, Elsevier, vol. 430(C), pages 126-135.
    12. Sumedha W Karmarkar & Kathleen M Bottum & Stacey L Krager & Shelley A Tischkau, 2011. "ERK/MAPK Is Essential for Endogenous Neuroprotection in SCN2.2 Cells," PLOS ONE, Public Library of Science, vol. 6(8), pages 1-13, August.
    13. O. Slaby & S. Sager & O. S. Shaik & U. Kummer & D. Lebiedz, 2007. "Optimal control of self-organized dynamics in cellular signal transduction," Mathematical and Computer Modelling of Dynamical Systems, Taylor & Francis Journals, vol. 13(5), pages 487-502, October.
    14. Michal Dudek & Dharshika R. J. Pathiranage & Beatriz Bano-Otalora & Anna Paszek & Natalie Rogers & Cátia F. Gonçalves & Craig Lawless & Dong Wang & Zhuojing Luo & Liu Yang & Farshid Guilak & Judith A., 2023. "Mechanical loading and hyperosmolarity as a daily resetting cue for skeletal circadian clocks," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    15. René Carmona & Christy V. Graves, 2020. "Jet Lag Recovery: Synchronization of Circadian Oscillators as a Mean Field Game," Dynamic Games and Applications, Springer, vol. 10(1), pages 79-99, March.
    16. Jing Wang & Haibo Di & Steven Laureys & Nantu Hu, 2018. "Circadian Rhythm of Patients with Disorders of Consciousness," Open Access Journal of Neurology & Neurosurgery, Juniper Publishers Inc., vol. 9(3), pages 60-61, November.
    17. Yan-Ying Wang & Wei-Wei Ma & I-Feng Peng, 2020. "Screening of sleep assisting drug candidates with a Drosophila model," PLOS ONE, Public Library of Science, vol. 15(7), pages 1-17, July.
    18. Hyeri Nam & Younghwan Lee & Boil Kim & Ji-Won Lee & Seohyeon Hwang & Hyun-Kyu An & Kyung Min Chung & Youngjin Park & Jihyun Hong & Kyungjin Kim & Eun-Kyoung Kim & Han Kyoung Choe & Seong-Woon Yu, 2022. "Presenilin 2 N141I mutation induces hyperactive immune response through the epigenetic repression of REV-ERBα," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    19. Li, Ying & Liu, Zengrong, 2016. "Coupling mechanism in the gate and oscillator model of the SCN," Physica A: Statistical Mechanics and its Applications, Elsevier, vol. 457(C), pages 62-72.
    20. Valerie L Harbour & Yuval Weigl & Barry Robinson & Shimon Amir, 2014. "Phase Differences in Expression of Circadian Clock Genes in the Central Nucleus of the Amygdala, Dentate Gyrus, and Suprachiasmatic Nucleus in the Rat," PLOS ONE, Public Library of Science, vol. 9(7), pages 1-9, July.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0076391. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.