IDEAS home Printed from https://ideas.repec.org/a/gam/jeners/v15y2022i22p8725-d978462.html
   My bibliography  Save this article

Taxonomic and Biochemical Characterization of Microalga Graesiella emersonii GEGS21 for Its Potential to Become Feedstock for Biofuels and Bioproducts

Author

Listed:
  • Nam Seon Kang

    (Department of Microbial Resources, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea)

  • Kichul Cho

    (Department of Microbial Resources, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea)

  • Sung Min An

    (Department of Microbial Resources, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea)

  • Eun Song Kim

    (Department of Microbial Resources, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea)

  • Hyunji Ki

    (Department of Microbial Resources, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea)

  • Chung Hyeon Lee

    (Department of Microbial Resources, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea)

  • Grace Choi

    (Department of Microbial Resources, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea)

  • Ji Won Hong

    (Department of Hydrogen and Renewable Energy, Kyungpook National University, Daegu 41566, Republic of Korea
    Advanced Bio-Resource Research Center, Kyungpook National University, Daegu 41566, Republic of Korea)

Abstract

Graesiella emersonii is a commercially exploitable source of bioactive compounds and biofuels with potential applications in microalgae-based industries. Despite this, little taxonomical information is available. Therefore, proper identification and characterization are needed for the sustainable utilization of isolated microalgae. In this study, an axenically isolated unicellular green alga from the Geumgang Estuary, Korea was investigated for its morphological, molecular, and biochemical characteristics. The morphological characteristics were typical of G. emersonii. Molecular phylogenetic analysis of the 18S rDNA sequence verified that the isolate belonged to G. emersonii and was subsequently named G. emersonii GEGS21. It was isolated from brackish water, and its optimal growth temperature, salinity, and light intensity were at 28–32 °C, 0 M NaCl, and 130–160 µmol m −2 s −1 , respectively. The strain thrived over a range of temperatures (5–40 °C) and withstood up to 0.5 M NaCl. The isolate was rich in omega-6 linoleic acid (C 18:2 n-6, 26.3%) and palmitic acid (C 16:0 , 27.5%). The fuel quality properties were determined, and biodiesel from GEGS21 could be used as a biodiesel blend. Value-added carotenoids lutein (1.5 mg g −1 dry cell weight, DCW) and neoxanthin (1.2 mg g −1 DCW) were biosynthesized as accessory pigments by this microalga. The biomass of this microalga may serve as feedstock for biodiesel production as well as producing valuable ω-6 and carotenoids.

Suggested Citation

  • Nam Seon Kang & Kichul Cho & Sung Min An & Eun Song Kim & Hyunji Ki & Chung Hyeon Lee & Grace Choi & Ji Won Hong, 2022. "Taxonomic and Biochemical Characterization of Microalga Graesiella emersonii GEGS21 for Its Potential to Become Feedstock for Biofuels and Bioproducts," Energies, MDPI, vol. 15(22), pages 1-24, November.
    • Handle: RePEc:gam:jeners:v:15:y:2022:i:22:p:8725-:d:978462
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1996-1073/15/22/8725/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1996-1073/15/22/8725/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Mandal, Madan Kumar & Chaurasia, Neha, 2021. "De novo transcriptomic analysis of Graesiella emersonii NC-M1 reveals differential genes expression in cell proliferation and lipid production under glucose and salt supplemented condition," Renewable Energy, Elsevier, vol. 179(C), pages 2004-2015.
    2. Nam Seon Kang & Eun Song Kim & Jung A Lee & Kyeong Mi Kim & Min Seok Kwak & Moongeun Yoon & Ji Won Hong, 2020. "First Report of the Dinoflagellate Genus Effrenium in the East Sea of Korea: Morphological, Genetic, and Fatty Acid Characteristics," Sustainability, MDPI, vol. 12(9), pages 1-16, May.
    3. Ramadhas, A.S. & Muraleedharan, C. & Jayaraj, S., 2005. "Performance and emission evaluation of a diesel engine fueled with methyl esters of rubber seed oil," Renewable Energy, Elsevier, vol. 30(12), pages 1789-1800.
    4. Abo El-Khair B. El-Sayed & Nashwa A. Fetyan & Farahat S. Moghanm & Mohssen Elbagory & Fatma M. Ibrahim & Mahmoud W. Sadik & Mohamed S. Shokr, 2022. "Biomass Fatty Acid Profile and Fuel Property Prediction of Bagasse Waste Grown Nannochloropsis oculata," Agriculture, MDPI, vol. 12(8), pages 1-11, August.
    5. Pomeroy, Brett & Grilc, Miha & Likozar, Blaž, 2022. "Artificial neural networks for bio-based chemical production or biorefining: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 153(C).
    6. Muhammad Aminul Islam & Marie Magnusson & Richard J. Brown & Godwin A. Ayoko & Md. Nurun Nabi & Kirsten Heimann, 2013. "Microalgal Species Selection for Biodiesel Production Based on Fuel Properties Derived from Fatty Acid Profiles," Energies, MDPI, vol. 6(11), pages 1-27, October.
    7. Lourdes Orejuela-Escobar & Arleth Gualle & Valeria Ochoa-Herrera & George P. Philippidis, 2021. "Prospects of Microalgae for Biomaterial Production and Environmental Applications at Biorefineries," Sustainability, MDPI, vol. 13(6), pages 1-19, March.
    8. Kevin R. Arrigo, 2005. "Erratum: Marine microorganisms and global nutrient cycles," Nature, Nature, vol. 438(7064), pages 122-122, November.
    9. Goembira, Fadjar & Saka, Shiro, 2015. "Advanced supercritical Methyl acetate method for biodiesel production from Pongamia pinnata oil," Renewable Energy, Elsevier, vol. 83(C), pages 1245-1249.
    10. Kevin R. Arrigo, 2005. "Marine microorganisms and global nutrient cycles," Nature, Nature, vol. 437(7057), pages 349-355, September.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Islam, Muhammad Aminul & Heimann, Kirsten & Brown, Richard J., 2017. "Microalgae biodiesel: Current status and future needs for engine performance and emissions," Renewable and Sustainable Energy Reviews, Elsevier, vol. 79(C), pages 1160-1170.
    2. Toby Kenney & Hong Gu & Tianshu Huang, 2021. "Poisson PCA: Poisson measurement error corrected PCA, with application to microbiome data," Biometrics, The International Biometric Society, vol. 77(4), pages 1369-1384, December.
    3. Thomas J. Browning & C. Mark Moore, 2023. "Global analysis of ocean phytoplankton nutrient limitation reveals high prevalence of co-limitation," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    4. Nils Giordano & Marinna Gaudin & Camille Trottier & Erwan Delage & Charlotte Nef & Chris Bowler & Samuel Chaffron, 2024. "Genome-scale community modelling reveals conserved metabolic cross-feedings in epipelagic bacterioplankton communities," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    5. Arumugam, A. & Thulasidharan, D. & Jegadeesan, Gautham B., 2018. "Process optimization of biodiesel production from Hevea brasiliensis oil using lipase immobilized on spherical silica aerogel," Renewable Energy, Elsevier, vol. 116(PA), pages 755-761.
    6. Gera, Anitha & Gayathri, R & Ezhilarasan, P & Ranga Rao, V & Ramana Murthy, M V, 2023. "Coupled physical-biogeochemical simulations of upwelling, ecological response to fresh water," Ecological Modelling, Elsevier, vol. 476(C).
    7. Auguères, Anne-Sophie & Loreau, Michel, 2016. "Biotic regulation of non-limiting nutrient pools and coupling of biogeochemical cycles," Ecological Modelling, Elsevier, vol. 334(C), pages 1-7.
    8. Haşimoğlu, Can & Ciniviz, Murat & Özsert, İbrahim & İçingür, Yakup & Parlak, Adnan & Sahir Salman, M., 2008. "Performance characteristics of a low heat rejection diesel engine operating with biodiesel," Renewable Energy, Elsevier, vol. 33(7), pages 1709-1715.
    9. Vallinayagam, R. & Vedharaj, S. & Yang, W.M. & Roberts, W.L. & Dibble, R.W., 2015. "Feasibility of using less viscous and lower cetane (LVLC) fuels in a diesel engine: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 51(C), pages 1166-1190.
    10. Piotr Łagowski & Grzegorz Wcisło & Dariusz Kurczyński, 2022. "Comparison of the Combustion Process Parameters in a Diesel Engine Powered by Second-Generation Biodiesel Compared to the First-Generation Biodiesel," Energies, MDPI, vol. 15(18), pages 1-21, September.
    11. Venu, Harish & Raju, V. Dhana & Subramani, Lingesan & Appavu, Prabhu, 2020. "Experimental assessment on the regulated and unregulated emissions of DI diesel engine fuelled with Chlorella emersonii methyl ester (CEME)," Renewable Energy, Elsevier, vol. 151(C), pages 88-102.
    12. Singh, Paramvir & Chauhan, S.R. & Goel, Varun, 2018. "Assessment of diesel engine combustion, performance and emission characteristics fuelled with dual fuel blends," Renewable Energy, Elsevier, vol. 125(C), pages 501-510.
    13. Çelikten, İsmet & Mutlu, Emre & Solmaz, Hamit, 2012. "Variation of performance and emission characteristics of a diesel engine fueled with diesel, rapeseed oil and hazelnut oil methyl ester blends," Renewable Energy, Elsevier, vol. 48(C), pages 122-126.
    14. Savvas L. Douvartzides & Nikolaos D. Charisiou & Kyriakos N. Papageridis & Maria A. Goula, 2019. "Green Diesel: Biomass Feedstocks, Production Technologies, Catalytic Research, Fuel Properties and Performance in Compression Ignition Internal Combustion Engines," Energies, MDPI, vol. 12(5), pages 1-41, February.
    15. Hoang-Tuong Nguyen Hao & Obulisamy Parthiba Karthikeyan & Kirsten Heimann, 2015. "Bio-Refining of Carbohydrate-Rich Food Waste for Biofuels," Energies, MDPI, vol. 8(7), pages 1-15, June.
    16. Mofijur, M. & Atabani, A.E. & Masjuki, H.H. & Kalam, M.A. & Masum, B.M., 2013. "A study on the effects of promising edible and non-edible biodiesel feedstocks on engine performance and emissions production: A comparative evaluation," Renewable and Sustainable Energy Reviews, Elsevier, vol. 23(C), pages 391-404.
    17. Zhu, Yixin & Xu, Jianchu & Li, Qiaohong & Mortimer, Peter E., 2014. "Investigation of rubber seed yield in Xishuangbanna and estimation of rubber seed oil based biodiesel potential in Southeast Asia," Energy, Elsevier, vol. 69(C), pages 837-842.
    18. Azad, A.K. & Rasul, M.G. & Khan, M.M.K. & Sharma, Subhash C. & Mofijur, M. & Bhuiya, M.M.K., 2016. "Prospects, feedstocks and challenges of biodiesel production from beauty leaf oil and castor oil: A nonedible oil sources in Australia," Renewable and Sustainable Energy Reviews, Elsevier, vol. 61(C), pages 302-318.
    19. Srinivasan Senthil Kumar & K. Rajan & Vinayagam Mohanavel & Manickam Ravichandran & Parvathy Rajendran & Ahmad Rashedi & Abhishek Sharma & Sher Afghan Khan & Asif Afzal, 2021. "Combustion, Performance, and Emission Behaviors of Biodiesel Fueled Diesel Engine with the Impact of Alumina Nanoparticle as an Additive," Sustainability, MDPI, vol. 13(21), pages 1-19, November.
    20. Bhale, Purnanand Vishwanathrao & Deshpande, Nishikant V. & Thombre, Shashikant B., 2009. "Improving the low temperature properties of biodiesel fuel," Renewable Energy, Elsevier, vol. 34(3), pages 794-800.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jeners:v:15:y:2022:i:22:p:8725-:d:978462. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.