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Screening of Fungal Strains for Cellulolytic and Xylanolytic Activities Production and Evaluation of Brewers’ Spent Grain as Substrate for Enzyme Production by Selected Fungi

Author

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  • Rossana Liguori

    (Department of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy)

  • Anna Pennacchio

    (Department of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy)

  • Luciana Porto de Souza Vandenberghe

    (Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná Coronel Francisco H. Dos Santos Avenue, 210, Curitiba 81531-980, Brazil)

  • Addolorata De Chiaro

    (Department of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy)

  • Leila Birolo

    (Department of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy)

  • Carlos Ricardo Soccol

    (Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná Coronel Francisco H. Dos Santos Avenue, 210, Curitiba 81531-980, Brazil)

  • Vincenza Faraco

    (Department of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy)

Abstract

Brewer’s spent grain (BSG), the solid residue of beer production, is attracting significant attention as raw material for the production of added value substances, since until recently it was mainly used as animal feed or deposited in landfills, causing serious environmental problems. Therefore, this work aimed at developing a bioprocess using BSG as a substrate for the production of cellulases and xylanases for waste saccharification and bioenergy production. Different fungi were analyzed for their cellulolytic and xylanolytic abilities, through a first screening on solid media by assessment of fungal growth and enzyme production on agar containing carboxylmethylcellulose or xylan as the sole carbon source, respectively. The best cellulase and xylanase producers were subjected to quantitative evaluation of enzyme production in liquid cultures. Aspergillus niger LPB-334 was selected for its ability to produce cellulase and xylanase at high levels and it was cultivated on BSG by solid state fermentation. The cellulase production reached a maximum of 118.04 ± 8.4 U/g of dry substrate after 10 days of fermentation, while a maximum xylanase production of 1315.15 ± 37.5 U/g of dry substrate was reached after 4 days. Preliminary characterization of cellulase and xylanase activities and identification of the enzymes responsible were carried out.

Suggested Citation

  • Rossana Liguori & Anna Pennacchio & Luciana Porto de Souza Vandenberghe & Addolorata De Chiaro & Leila Birolo & Carlos Ricardo Soccol & Vincenza Faraco, 2021. "Screening of Fungal Strains for Cellulolytic and Xylanolytic Activities Production and Evaluation of Brewers’ Spent Grain as Substrate for Enzyme Production by Selected Fungi," Energies, MDPI, vol. 14(15), pages 1-17, July.
    • Handle: RePEc:gam:jeners:v:14:y:2021:i:15:p:4443-:d:599786
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    References listed on IDEAS

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    1. Amore, Antonella & Faraco, Vincenza, 2012. "Potential of fungi as category I Consolidated BioProcessing organisms for cellulosic ethanol production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 16(5), pages 3286-3301.
    2. Rossana Liguori & Carlos Ricardo Soccol & Luciana Porto de Souza Vandenberghe & Adenise Lorenci Woiciechowski & Vincenza Faraco, 2015. "Second Generation Ethanol Production from Brewers’ Spent Grain," Energies, MDPI, vol. 8(4), pages 1-12, March.
    3. Richard Ahorsu & Francesc Medina & Magda Constantí, 2018. "Significance and Challenges of Biomass as a Suitable Feedstock for Bioenergy and Biochemical Production: A Review," Energies, MDPI, vol. 11(12), pages 1-19, December.
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