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Polyhydroxyalkanoated-Rich Microbial Cells from Bio-Based Volatile Fatty Acids as Potential Ingredient for Aquaculture Feed

Author

Listed:
  • Alice Botturi

    (Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy)

  • Federico Battista

    (Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy)

  • Marco Andreolli

    (Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy)

  • Filippo Faccenda

    (Technology Transfer Centre, Fondazione Edmund Mach, via E. Mach 1, 38010 San Michele all’Adige, Italy)

  • Salvatore Fusco

    (Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy)

  • David Bolzonella

    (Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy)

  • Silvia Lampis

    (Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy)

  • Nicola Frison

    (Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy)

Abstract

In this study, the production of polyhydroxyalkanoated PHA-rich microbial biomass as a novel feed additive in aquaculture was investigated at a lab-scale. Bio-based volatile fatty acids (VFAs), obtained from the acidogenic fermentation of agricultural residues in existing anaerobic digestion plants, were used as carbon and energy to cultivate the PHA-rich microbial biomass. The experimental activities were carried out using Thauera sp. Sel9 as pure strain, which was grown in a continuous stirred-tank reactor (CSTR) operated at three different hydraulic retention times (HRT). The highest productivity obtained of biomass cells was 0.69 g/L day, operating at one day HRT while the observed PHAs production yield was 0.14 gPHA/g soluble COD removed. At these conditions, the PHA concentration in the microbial cells was 41%. Although the sulfur amino acids were available at high concentrations and above the typical concentration found in fishmeal, the amino acids profile of the obtained biomass revealed a lack of histidine and threonine. A preliminary economic analysis showed that the production of a novel source of feed additive from the conversion of agro-residues could give higher benefits in terms of revenues compared to the production of biogas production through anaerobic digestion.

Suggested Citation

  • Alice Botturi & Federico Battista & Marco Andreolli & Filippo Faccenda & Salvatore Fusco & David Bolzonella & Silvia Lampis & Nicola Frison, 2020. "Polyhydroxyalkanoated-Rich Microbial Cells from Bio-Based Volatile Fatty Acids as Potential Ingredient for Aquaculture Feed," Energies, MDPI, vol. 14(1), pages 1-9, December.
    • Handle: RePEc:gam:jeners:v:14:y:2020:i:1:p:38-:d:467178
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    References listed on IDEAS

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    1. Harun, Razif & Singh, Manjinder & Forde, Gareth M. & Danquah, Michael K., 2010. "Bioprocess engineering of microalgae to produce a variety of consumer products," Renewable and Sustainable Energy Reviews, Elsevier, vol. 14(3), pages 1037-1047, April.
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