Fermentation Regulation: Revealing Bacterial Community Structure, Symbiotic Networks to Function and Pathogenic Risk in Corn Stover Silage

Improving agricultural by-product utilization can alleviate tropical feed shortages. This study used corn stover (CS, Zea mays L.) at the maturity stage as the material, with four silage treatments: control, lactic acid bacteria (LAB, Lactiplantibacillus plantarum ), cellulase (AC, Acremonium cellulolyticus ), and LAB+AC. After 60 days fermentation in plastic drum silos, the silos were opened for sampling. PacBio single-molecule real-time sequencing technology was used to study bacterial community structure, symbiotic network functionality, and pathogenic risk to clarify CS fermentation regulatory mechanisms. The CS contained 59.9% neutral detergent fiber and 7.1% crude protein. Additive-treated silages showed better quality than the control: higher lactic acid (1.64–1.83% dry matter, DM), lower pH (3.62–3.82), and reduced ammonia nitrogen (0.54–0.81% DM). Before ensiling, the CS was dominated by Gram-negative Rhizobium larrymoorei (16.30% of the total bacterial community). Functional prediction indicated that the microbial metabolism activity in diverse environments was strong, and the proportion of potential pathogens was relatively high (14.69%). After ensiling, Lactiplantibacillus plantarum as Gram-positive bacteria were the dominant species in all the silages (58.39–84.34% of the total bacterial community). Microbial additives facilitated the establishment of a symbiotic microbial network, where Lactiplantibacillus occupied a dominant position ( p < 0.01). In addition, functional predictions showed an increase in the activity of the starch and sucrose metabolism and a decrease in the proportion of potential pathogens (0.61–1.95%). Among them, the synergistic effect of LAB and AC inoculants optimized the silage effect of CS. This study confirmed that CS is a potential high-quality roughage resource, and the application of silage technology can provide a scientific basis for the efficient utilization of feed resources and the stable development of animal husbandry in the tropics.