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Combination of Mechanical/Physical Pretreatments with Trypsin or Pancreatin on Arthrospira platensis Protein Degradation

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  • Mónica M. Costa

    (CIISA—Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Av. da Universidade Técnica, 1300-477 Lisboa, Portugal
    Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Faculdade de Medicina Veterinária, Universidade de Lisboa, Av. da Universidade Técnica, 1300-477 Lisboa, Portugal
    These authors contributed equally to this work.)

  • Maria P. Spínola

    (CIISA—Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Av. da Universidade Técnica, 1300-477 Lisboa, Portugal
    Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Faculdade de Medicina Veterinária, Universidade de Lisboa, Av. da Universidade Técnica, 1300-477 Lisboa, Portugal
    These authors contributed equally to this work.)

  • José A. M. Prates

    (CIISA—Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Av. da Universidade Técnica, 1300-477 Lisboa, Portugal
    Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Faculdade de Medicina Veterinária, Universidade de Lisboa, Av. da Universidade Técnica, 1300-477 Lisboa, Portugal)

Abstract

Arthrospira platensis contains high protein content and quality, which makes it a promising feed source for livestock animals. However, this microalga presents a recalcitrant peptidoglycan cell wall, and its main proteins form protein-pigment complexes attached to the algal thylakoid membrane. The purpose of the present study was to evaluate the effect of mechanical/physical pretreatments (bead milling, extrusion, freeze-drying, heating, microwave and sonication) combined with commercial enzymes (trypsin or pancreatin) on the degradation of A. platensis proteins. Protein degradation was assessed through the quantification of total protein and protein fractions (18–26 kDa, 40–48 kDa and others) on SDS-PAGE gels and the evaluation of the coefficient of protein degradation (CPD). The results showed that no significant differences were found among treatments for CPD values, except for an increase related to 18–26 kDa protein (phycocyanin subunits) with extrusion combined with pancreatin. In addition, extrusion and microwave caused a decrease of total protein in algal supernatant probably due to a denaturation/aggregation and reduction of solubility. Overall, extrusion is a promising pretreatment for A. platensis cell wall disruption and protein bioaccessibility. Further studies could elucidate how the effect of extrusion on protein solubility affects the activity of peptidases on protein degradation.

Suggested Citation

  • Mónica M. Costa & Maria P. Spínola & José A. M. Prates, 2023. "Combination of Mechanical/Physical Pretreatments with Trypsin or Pancreatin on Arthrospira platensis Protein Degradation," Agriculture, MDPI, vol. 13(1), pages 1-12, January.
    • Handle: RePEc:gam:jagris:v:13:y:2023:i:1:p:198-:d:1034198
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    References listed on IDEAS

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    1. McMillan, Jonathan R. & Watson, Ian A. & Ali, Mehmood & Jaafar, Weaam, 2013. "Evaluation and comparison of algal cell disruption methods: Microwave, waterbath, blender, ultrasonic and laser treatment," Applied Energy, Elsevier, vol. 103(C), pages 128-134.
    2. Wang, Meng & Cheng, He & Chen, Shibao & Wen, Shumei & Wu, Xia & Zhang, Dongmei & Yuan, Qipeng & Cong, Wei, 2018. "Microalgal cell disruption via extrusion for the production of intracellular valuables," Energy, Elsevier, vol. 142(C), pages 339-345.
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    Cited by:

    1. Maria P. Spínola & Mónica M. Costa & José A. M. Prates, 2023. "Effect of Selected Mechanical/Physical Pre-Treatments on Chlorella vulgaris Protein Solubility," Agriculture, MDPI, vol. 13(7), pages 1-13, June.

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