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Comparative study on ethanol production from pretreated sugarcane bagasse using immobilized Saccharomyces cerevisiae on various matrices

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  • Singh, Anita
  • Sharma, Punita
  • Saran, Alok Kumar
  • Singh, Namita
  • Bishnoi, Narsi R.

Abstract

Microwave alkali pretreated sugarcane bagasse was used as a substrate for production of cellulolytic enzymes, needed for biomass hydrolysis. The pretreated sugarcane bagasse was enzymatic hydrolyzed by crude unprocessed enzymes cellulase (Filter paper activity 9.4 FPU/g), endoglucanase (carboxymethylcellulase, 148 IU/g), β-glucosidase (116 IU/g) and xylanase (201 IU/g) produced by Aspergillus flavus using pretreated sugarcane bagasse as substrate under solid state fermentation. Concentrated enzymatic hydrolyzate was used for ethanol production using Saccharomyces cerevisiae immobilized on various matrices. The yield of ethanol was 0.44 gp/gs in case of yeast immobilized sugarcane bagasse, 0.38 gp/gs using Ca-alginate and 0.33 gp/gs using agar-agar as immobilization matrices. The immobilized yeast studied up to 10 cycles in case of immobilized sugarcane bagasse and up to 4 cycles in case of agar-agar and calcium alginate for ethanol production under repeated batch fermentation study.

Suggested Citation

  • Singh, Anita & Sharma, Punita & Saran, Alok Kumar & Singh, Namita & Bishnoi, Narsi R., 2013. "Comparative study on ethanol production from pretreated sugarcane bagasse using immobilized Saccharomyces cerevisiae on various matrices," Renewable Energy, Elsevier, vol. 50(C), pages 488-493.
  • Handle: RePEc:eee:renene:v:50:y:2013:i:c:p:488-493
    DOI: 10.1016/j.renene.2012.07.003
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    1. Behera, Shuvashish & Kar, Shaktimay & Mohanty, Rama Chandra & Ray, Ramesh Chandra, 2010. "Comparative study of bio-ethanol production from mahula (Madhuca latifolia L.) flowers by Saccharomyces cerevisiae cells immobilized in agar agar and Ca-alginate matrices," Applied Energy, Elsevier, vol. 87(1), pages 96-100, January.
    2. Yu, Jianliang & Yue, Guojun & Zhong, Jing & Zhang, Xu & Tan, Tianwei, 2010. "Immobilization of Saccharomyces cerevisiae to modified bagasse for ethanol production," Renewable Energy, Elsevier, vol. 35(6), pages 1130-1134.
    3. Sukumaran, Rajeev K. & Singhania, Reeta Rani & Mathew, Gincy Marina & Pandey, Ashok, 2009. "Cellulase production using biomass feed stock and its application in lignocellulose saccharification for bio-ethanol production," Renewable Energy, Elsevier, vol. 34(2), pages 421-424.
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