IDEAS home Printed from https://ideas.repec.org/a/eee/renene/v242y2025ics0960148125001119.html
   My bibliography  Save this article

The enzyme cocktail produced by endophytic Chaetomium globosum unlocks in situ production of sugars and shows great potential application in biorefinery

Author

Listed:
  • Pan, Yinghao
  • Lu, Yao
  • Dong, Yamin
  • Sun, Ying
  • Tang, Ruiqi
  • Xiao, Yiwen
  • Gao, Boliang
  • Zhu, Du

Abstract

The approach producing sugars in situ has been considered as a viable way to significantly improve the economics of biorefinery. However, the implementation of in situ sugar production relies on highly efficient enzymes and their affordable, convenient production. We previously reported a lignocellulolytic enzyme cocktail (CG3) from Chaetomium globosum DX-THS3 which showed high hydrolysis performance for raw rice straw, revealing great potential to develop in situ production sugars. Herein, CG3 production process through solid-state fermentation was evaluated and verified at pilot scale in tray fermenters. By exploring solid-state inoculation, enzymatic performance comparable to conventional liquid-state inoculation was obtained in a shorter fermentation period (from 12 to 6 days). Compared to Cellic CTec 2, CG3 exhibited superior hydrolytic efficiency for various lignocellulosic biomasses, especially for untreated lignocellulose. Pretreated agricultural wastes hydrolyzed with CG3 high-efficiently yielded glucose (0.35–0.40 g/g dry biomass). Additionally, bioethanol production from Saccharomyces cerevisiae using CG3-generated sugars reached 41.0–52.9 g/L without detoxification. Overall, our study presents a promising enzyme cocktail for saccharifying lignocellulosic biomass and suggests an integrated approach for in situ enzyme and sugar production.

Suggested Citation

  • Pan, Yinghao & Lu, Yao & Dong, Yamin & Sun, Ying & Tang, Ruiqi & Xiao, Yiwen & Gao, Boliang & Zhu, Du, 2025. "The enzyme cocktail produced by endophytic Chaetomium globosum unlocks in situ production of sugars and shows great potential application in biorefinery," Renewable Energy, Elsevier, vol. 242(C).
    • Handle: RePEc:eee:renene:v:242:y:2025:i:c:s0960148125001119
    DOI: 10.1016/j.renene.2025.122449
    as

    Download full text from publisher

    File URL: http://www.sciencedirect.com/science/article/pii/S0960148125001119
    Download Restriction: Full text for ScienceDirect subscribers only
    File URL: https://libkey.io/10.1016/j.renene.2025.122449?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. da Silva, Francinaldo Leite & de Oliveira Campos, Alan & dos Santos, Davi Alves & de Oliveira Júnior, Sérgio Dantas & de Araújo Padilha, Carlos Eduardo & de Sousa Junior, Francisco Caninde & de Macedo, 2018. "Pretreatments of Carnauba (Copernicia prunifera) straw residue for production of cellulolytic enzymes by Trichorderma reesei CCT-2768 by solid state fermentation," Renewable Energy, Elsevier, vol. 116(PA), pages 299-308.
    2. Qiao, Hui & Han, Mingyang & Ouyang, Shuiping & Zheng, Zhaojuan & Ouyang, Jia, 2022. "An integrated lignocellulose biorefinery process: Two-step sequential treatment with formic acid for efficiently producing ethanol and furfural from corn cobs," Renewable Energy, Elsevier, vol. 191(C), pages 775-784.
    3. Ezeilo, Uchenna R. & Wahab, Roswanira Abdul & Mahat, Naji Arafat, 2020. "Optimization studies on cellulase and xylanase production by Rhizopus oryzae UC2 using raw oil palm frond leaves as substrate under solid state fermentation," Renewable Energy, Elsevier, vol. 156(C), pages 1301-1312.
    4. Appiah-Nkansah, Nana Baah & Li, Jun & Rooney, William & Wang, Donghai, 2019. "A review of sweet sorghum as a viable renewable bioenergy crop and its techno-economic analysis," Renewable Energy, Elsevier, vol. 143(C), pages 1121-1132.
    5. Hemansi, & Gupta, Rishi & Aswal, Vinod K. & Saini, Jitendra Kumar, 2020. "Sequential dilute acid and alkali deconstruction of sugarcane bagasse for improved hydrolysis: Insight from small angle neutron scattering (SANS)," Renewable Energy, Elsevier, vol. 147(P1), pages 2091-2101.
    6. Awasthi, Mukesh Kumar & Sindhu, Raveendran & Sirohi, Ranjna & Kumar, Vinod & Ahluwalia, Vivek & Binod, Parameswaran & Juneja, Ankita & Kumar, Deepak & Yan, Binghua & Sarsaiya, Surendra & Zhang, Zengqi, 2022. "Agricultural waste biorefinery development towards circular bioeconomy," Renewable and Sustainable Energy Reviews, Elsevier, vol. 158(C).
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Bhattacharya, Raikamal & Arora, Sidharth & Ghosh, Sanjoy, 2022. "Utilization of waste pine needles for the production of cellulolytic enzymes in a solid state fermentation bioreactor and high calorific value fuel pellets from fermented residue: Towards a biorefiner," Renewable Energy, Elsevier, vol. 195(C), pages 1064-1076.
    2. Ezeilo, Uchenna R. & Wahab, Roswanira Abdul & Mahat, Naji Arafat, 2020. "Optimization studies on cellulase and xylanase production by Rhizopus oryzae UC2 using raw oil palm frond leaves as substrate under solid state fermentation," Renewable Energy, Elsevier, vol. 156(C), pages 1301-1312.
    3. Kiehbadroudinezhad, Mohammadali & Hosseinzadeh-Bandbafha, Homa & Pan, Junting & Peng, Wanxi & Wang, Yajing & Aghbashlo, Mortaza & Tabatabaei, Meisam, 2023. "The potential of aquatic weed as a resource for sustainable bioenergy sources and bioproducts production," Energy, Elsevier, vol. 278(PA).
    4. Duan, Yumin & Wang, Zhi & Ganeshan, Prabakaran & Sar, Taner & Xu, Suyun & Rajendran, Karthik & Sindhu, Raveendran & Binod, Parameswaran & Pandey, Ashok & Zhang, Zengqiang & Taherzadeh, Mohammad J. & A, 2025. "Anaerobic digestion in global bio-energy production for sustainable bioeconomy: Potential and research challenges," Renewable and Sustainable Energy Reviews, Elsevier, vol. 208(C).
    5. Malherbe, Sarel J.M. & Cripwell, Rosemary A. & Favaro, Lorenzo & van Zyl, Willem H. & Viljoen-Bloom, Marinda, 2023. "Triticale and sorghum as feedstock for bioethanol production via consolidated bioprocessing," Renewable Energy, Elsevier, vol. 206(C), pages 498-505.
    6. Tinôco, Daniel & Genier, Hugo Leonardo André & da Silveira, Wendel Batista, 2021. "Technology valuation of cellulosic ethanol production by Kluyveromyces marxianus CCT 7735 from sweet sorghum bagasse at elevated temperatures," Renewable Energy, Elsevier, vol. 173(C), pages 188-196.
    7. Prakash Kumar Sarangi & Priti Pal & Akhilesh Kumar Singh & Uttam Kumar Sahoo & Piotr Prus, 2024. "Food Waste to Food Security: Transition from Bioresources to Sustainability," Resources, MDPI, vol. 13(12), pages 1-24, November.
    8. Tong, Wenyao & Chu, Qiulu & Li, Jin & Xie, Xinyu & Wang, Jing & Jin, Yongcan & Wu, Shufang & Hu, Jinguang & Song, Kai, 2022. "Insight into understanding sequential two-stage pretreatment on modifying lignin physiochemical properties and improving holistic utilization of renewable lignocellulose biomass," Renewable Energy, Elsevier, vol. 187(C), pages 123-134.
    9. Weiwei Wang, 2023. "Integrated Assessment of Economic Supply and Environmental Effects of Biomass Co-Firing in Coal Power Plants: A Case Study of Jiangsu, China," Energies, MDPI, vol. 16(6), pages 1-22, March.
    10. Baramee, Sirilak & Siriatcharanon, Ake-kavitch & Ketbot, Prattana & Teeravivattanakit, Thitiporn & Waeonukul, Rattiya & Pason, Patthra & Tachaapaikoon, Chakrit & Ratanakhanokchai, Khanok & Phitsuwan, , 2020. "Biological pretreatment of rice straw with cellulase-free xylanolytic enzyme-producing Bacillus firmus K-1: Structural modification and biomass digestibility," Renewable Energy, Elsevier, vol. 160(C), pages 555-563.
    11. Hongshen Li & Hongrui Liu & Yufang Li & Jilin Nan & Chen Shi & Shizhong Li, 2021. "Combined Vapor Permeation and Continuous Solid-State Distillation for Energy-Efficient Bioethanol Production," Energies, MDPI, vol. 14(8), pages 1-15, April.
    12. Poomani, Merlin Sobia & Mariappan, Iyyadurai & Muthan, Krishnaveni & Subramanian, Venkatesh, 2024. "Insights of Pichia kudriavzevii SVMS2019 for cellulase production and fermentation into ethanol," Renewable Energy, Elsevier, vol. 225(C).
    13. Ben Atitallah, Imen & Ntaikou, Ioanna & Antonopoulou, Georgia & Alexandropoulou, Maria & Brysch-Herzberg, Michael & Nasri, Moncef & Lyberatos, Gerasimos & Mechichi, Tahar, 2020. "Evaluation of the non-conventional yeast strain Wickerhamomyces anomalus (Pichia anomala) X19 for enhanced bioethanol production using date palm sap as renewable feedstock," Renewable Energy, Elsevier, vol. 154(C), pages 71-81.
    14. de Rossi, Eduardo & Tavares, Maria Herminia Ferreira & Teleken, Joel Gustavo & Cremonez, Paulo André & Christ, Divair & Gomes, Simone Damasceno & Bariccatti, Reinaldo Aparecido, 2022. "Production of biogas by microorganisms with saccharine sorghum straw as substrate with or without alkaline and citric pretreatment," Renewable Energy, Elsevier, vol. 197(C), pages 617-626.
    15. Can Cui & Cancan Yan & Ailin Wang & Cui Chen & Dan Chen & Shiwei Liu & Lu Li & Qiong Wu & Yue Liu & Yuxiang Liu & Genkuo Nie & Xiaoqing Jiang & Shuangxi Nie & Shuangquan Yao & Hailong Yu, 2023. "Understanding the Inhibition Mechanism of Lignin Adsorption to Cellulase in Terms of Changes in Composition and Conformation of Free Enzymes," Sustainability, MDPI, vol. 15(7), pages 1-12, March.
    16. Hassan El-Ramady & Eric C. Brevik & Yousry Bayoumi & Tarek A. Shalaby & Mohammed E. El-Mahrouk & Naglaa Taha & Heba Elbasiouny & Fathy Elbehiry & Megahed Amer & Neama Abdalla & József Prokisch & Svein, 2022. "An Overview of Agro-Waste Management in Light of the Water-Energy-Waste Nexus," Sustainability, MDPI, vol. 14(23), pages 1-30, November.
    17. Wang, Peng & Su, Yan & Tang, Wei & Huang, Caoxing & Lai, Chenhuan & Ling, Zhe & Yong, Qiang, 2022. "Revealing enzymatic digestibility of kraft pretreated larch based on a comprehensive analysis of substrate-related factors," Renewable Energy, Elsevier, vol. 199(C), pages 1461-1468.
    18. Mohd Safaai, Nor Sharliza & Pang, Shusheng, 2021. "Pyrolysis kinetics of chemically treated and torrefied radiata pine identified through thermogravimetric analysis," Renewable Energy, Elsevier, vol. 175(C), pages 200-213.
    19. Mondal, Sourav & Neogi, Swati & Chakraborty, Saikat, 2024. "Optimization of reactor parameters for amplifying synergy in enzymatic co-hydrolysis and microbial co-fermentation of lignocellulosic agro-residues," Renewable Energy, Elsevier, vol. 225(C).
    20. Zhang, Kai & Yin, Kedong & Yang, Wendong, 2022. "Predicting bioenergy power generation structure using a newly developed grey compositional data model: A case study in China," Renewable Energy, Elsevier, vol. 198(C), pages 695-711.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:eee:renene:v:242:y:2025:i:c:s0960148125001119. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Catherine Liu (email available below). General contact details of provider: http://www.journals.elsevier.com/renewable-energy .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.