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In-situ lignin modification in acid-alkaline combinational pretreatment on masson pine for efficient biomass saccharification

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  • Zhou, Dandan
  • Zhang, Zhiheng
  • Zhang, Shiyu
  • Meng, Di
  • Chu, Qiulu
  • Wang, Jing
  • Wu, Shufang
  • Hu, Jinguang

Abstract

The recalcitrance of softwood towards enzymatic hydrolysis is one of the major bottlenecks hindering its profitable use as feedstock for lignocellulosic biorefinery. This study presents double in-situ lignin modification in an acid-alkaline combinational pretreatment. Results indicated that complete delignification was not essential to achieve theoretical hydrolysis of cellulose. Alternatively, partial lignin removal accompanied by selective modification of the lignin gave rise to significantly inproved ease of enzymatic hydrolysis. Several analyses, including (GPC, 31P NMR, 2D HSQC NMR, QCM and DLS) were performed to reveal the underlying mechanism of the double in-situ lignin modification in enhancing ease of cellulose hydrolysis. Moreover, detaching behavior of lignin during enzymatic hydrolysis was also elucidated, which provided new insights into understanding the role lignin repolymerization inhibitors and combinational pretreatments played in improving enzymatic digestibility of lignocellulosic biomass.

Suggested Citation

  • Zhou, Dandan & Zhang, Zhiheng & Zhang, Shiyu & Meng, Di & Chu, Qiulu & Wang, Jing & Wu, Shufang & Hu, Jinguang, 2026. "In-situ lignin modification in acid-alkaline combinational pretreatment on masson pine for efficient biomass saccharification," Renewable Energy, Elsevier, vol. 257(C).
    • Handle: RePEc:eee:renene:v:257:y:2026:i:c:s0960148125024000
    DOI: 10.1016/j.renene.2025.124736
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    References listed on IDEAS

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    1. Hemansi, & Gupta, Rishi & Aswal, Vinod K. & Saini, Jitendra Kumar, 2020. "Sequential dilute acid and alkali deconstruction of sugarcane bagasse for improved hydrolysis: Insight from small angle neutron scattering (SANS)," Renewable Energy, Elsevier, vol. 147(P1), pages 2091-2101.
    2. Ying, Wenjun & Zhu, Junjun & Zhang, Junhua, 2025. "Improving enzymatic hydrolysis efficiency of highly recalcitrant Chinese fir biomass via hydrogen peroxide/acetic acid pretreatment and alkaline incubation," Renewable Energy, Elsevier, vol. 239(C).
    3. Yang, Luan & Zheng, Tianran & Huang, Chen & Yao, Jianfeng, 2022. "Using deep eutectic solvent pretreatment for enhanced enzymatic saccharification and lignin utilization of masson pine," Renewable Energy, Elsevier, vol. 195(C), pages 681-687.
    4. Chen, Zhengyu & Wang, Huan & Wei, Weiqi & Yuan, Zhaoyang, 2021. "Enhancing bagasse enzymatic hydrolysis through combination of ball-milling and LiCl/DMSO dissolution and regeneration," Renewable Energy, Elsevier, vol. 171(C), pages 994-1001.
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