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Evolution of microbial community structure during biohydrogen production process of palm oil anaerobic sludge

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  • Akhbari, Azam
  • Awalin, Lilik Jamilatul
  • Wen, Low Chin
  • Ali, Mohd Syukri
  • Ibrahim, Shaliza

Abstract

Anaerobic microbial communities and their functions can provide information about waste management practices, as well as reveal novel degradation metabolisms that can be utilized in wastewater treatment. This study was aimed towards sustainable palm oil industry with assessing the microbial evolution during the dark fermentative processing of palm oil anaerobic sludge and palm oil mill effluent (POME) in batch-mode over two-months with a distinct incubation time of 24 h. The process was designed using response surface methodology (RSM) by acclimatization of inoculum using POME with a chemical oxygen demand (COD) of 36 g-COD/l. The effect of mesophilic and thermophilic conditions and pH on COD removal, hydrogen content, and hydrogen yield (HY) were investigated. A potential sample was selected for microbial analysis after the first and second months of operation based on the maximum outputs achieved throughout the experiment. The bacterial community at pH 5.6 and temperature 55 °C with maximum hydrogen content of 64.13 %, and HY of 0.93 ml H2/g-COD was dominated by three phyla: Firmicutes, Actinobacteria, and Bacteroidota. The results obtained from high throughput pyrosequencing showed that how acclimatization period affects the microbial community structure towards Firmicutes phylum, which includes the majority of hydrogen-producing microorganisms.

Suggested Citation

  • Akhbari, Azam & Awalin, Lilik Jamilatul & Wen, Low Chin & Ali, Mohd Syukri & Ibrahim, Shaliza, 2024. "Evolution of microbial community structure during biohydrogen production process of palm oil anaerobic sludge," Renewable Energy, Elsevier, vol. 237(PB).
    • Handle: RePEc:eee:renene:v:237:y:2024:i:pb:s0960148124017452
    DOI: 10.1016/j.renene.2024.121677
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    References listed on IDEAS

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    1. Silva-Illanes, Fernando & Tapia-Venegas, Estela & Schiappacasse, M. Cristina & Trably, Eric & Ruiz-Filippi, Gonzalo, 2017. "Impact of hydraulic retention time (HRT) and pH on dark fermentative hydrogen production from glycerol," Energy, Elsevier, vol. 141(C), pages 358-367.
    2. Akhbari, Azam & Ibrahim, Shaliza & Ahmad, Muhammad Shakeel, 2023. "Optimization of up-flow velocity and feed flow rate in up-flow anaerobic sludge blanket fixed-film reactor on bio-hydrogen production from palm oil mill effluent," Energy, Elsevier, vol. 266(C).
    3. Łukajtis, Rafał & Hołowacz, Iwona & Kucharska, Karolina & Glinka, Marta & Rybarczyk, Piotr & Przyjazny, Andrzej & Kamiński, Marian, 2018. "Hydrogen production from biomass using dark fermentation," Renewable and Sustainable Energy Reviews, Elsevier, vol. 91(C), pages 665-694.
    4. Akhbari, Azam & Ibrahim, Shaliza & Ahmad, Muhammad Shakeel, 2023. "Feasibility of semi-pilot scale up-flow anaerobic sludge blanket fixed-film reactor for fermentative bio-hydrogen production from palm oil mill effluent," Renewable Energy, Elsevier, vol. 212(C), pages 612-620.
    5. Wong, Yee Meng & Wu, Ta Yeong & Juan, Joon Ching, 2014. "A review of sustainable hydrogen production using seed sludge via dark fermentation," Renewable and Sustainable Energy Reviews, Elsevier, vol. 34(C), pages 471-482.
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