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Evaluation of an adapted inhibitor-tolerant yeast strain for ethanol production from combined hydrolysate of softwood

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  • Tian, Shen
  • Zhu, Junyong
  • Yang, Xiushan

Abstract

In order to evaluate the potential of an adapted inhibitor-tolerant yeast strain developed in our lab to produce ethanol from softwood, the effect of furfural and HMF presented in defined medium and pretreatment hydrolysate on cell growth was investigated. And the efficiency of ethanol production from enzymatic hydrolysate mixed with pretreatment hydrolysate of softwood by bisulfite and sulfuric acid pretreatment process was reported. The results showed that in the combined treatments of the two inhibitors, cell growth was not affected at 1Â g/L each of furfural and HMF. When 3Â g/L each of furfural and HMF was applied, the adapted strain responded with an extended lag phase of 24Â h. Both in batch and fed-batch runs of combined hydrolysate fermentation, the final ethanol concentrations were above 20.0Â g/L and the ethanol yields (Yp/s) on the total amount of fermentable sugar presented in the pretreated materials were above 0.40Â g/g. It implies the great promise of the yeast strain for improving ethanol production from softwood due to its high ability of metabolizing inhibitor compounds of furfural and HMF.

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  • Tian, Shen & Zhu, Junyong & Yang, Xiushan, 2011. "Evaluation of an adapted inhibitor-tolerant yeast strain for ethanol production from combined hydrolysate of softwood," Applied Energy, Elsevier, vol. 88(5), pages 1792-1796, May.
  • Handle: RePEc:eee:appene:v:88:y:2011:i:5:p:1792-1796
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    References listed on IDEAS

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    1. Zheng, Yi & Pan, Zhongli & Zhang, Ruihong & Wang, Donghai, 2009. "Enzymatic saccharification of dilute acid pretreated saline crops for fermentable sugar production," Applied Energy, Elsevier, vol. 86(11), pages 2459-2465, November.
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    2. Ho, Cheng-Yu & Chang, Jui-Jen & Lee, Shih-Chi & Chin, Tsu-Yuan & Shih, Ming-Che & Li, Wen-Hsiung & Huang, Chieh-Chen, 2012. "Development of cellulosic ethanol production process via co-culturing of artificial cellulosomal Bacillus and kefir yeast," Applied Energy, Elsevier, vol. 100(C), pages 27-32.
    3. Wang, Tiejun & Li, Kai & Liu, Qiying & Zhang, Qing & Qiu, Songbai & Long, Jinxing & Chen, Lungang & Ma, Longlong & Zhang, Qi, 2014. "Aviation fuel synthesis by catalytic conversion of biomass hydrolysate in aqueous phase," Applied Energy, Elsevier, vol. 136(C), pages 775-780.
    4. Zhang, Xinghua & Wang, Tiejun & Ma, Longlong & Zhang, Qi & Huang, Xiaoming & Yu, Yuxiao, 2013. "Production of cyclohexane from lignin degradation compounds over Ni/ZrO2–SiO2 catalysts," Applied Energy, Elsevier, vol. 112(C), pages 533-538.
    5. Dong, Chengyu & Wang, Ying & Chan, Ka-Lai & Bhatia, Akanksha & Leu, Shao-Yuan, 2018. "Temperature profiling to maximize energy yield with reduced water input in a lignocellulosic ethanol biorefinery," Applied Energy, Elsevier, vol. 214(C), pages 63-72.

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