IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v16y2025i1d10.1038_s41467-025-62467-6.html
   My bibliography  Save this article

Disentangling extracellular current of electroactive bacteria with oblique-incidence reflection difference imaging

Author

Listed:
  • Shiwu Qian

    (Southwest University
    Ministry of Education
    Chongqing Key Laboratory of Battery Materials and Technology)

  • Chenyu Wang

    (Southwest University
    Chongqing Key Laboratory of Battery Materials and Technology)

  • Changxiang Fang

    (Southwest University
    Chongqing Key Laboratory of Battery Materials and Technology)

  • Meng Li

    (Southwest University
    Ministry of Education
    Chongqing Key Laboratory of Battery Materials and Technology)

  • Yan Qiao

    (Southwest University
    Chongqing Key Laboratory of Battery Materials and Technology)

  • Weihua Hu

    (Southwest University
    Ministry of Education
    Chongqing Key Laboratory of Battery Materials and Technology)

Abstract

Understanding the extracellular electron transfer (EET) process of electroactive bacteria is of great significance. It is critical yet challenging to differentiate the partial currents from direct (DET) and mediated electron transfer (MET) pathways in the integrated EET current. Herein the EET current of model exoelectrogen is successfully disentangled by using spatiotemporally-resolved oblique-incidence reflection difference (OIRD) technique coupled with polyaniline (PANI)-based dual electrode. The PANI film serves as an electron acceptor to translate the charge information into OIRD signals, enabling mapping of EET current. Upon complete reduction of PANI, the local EET current is switched off, and the soluble mediators are forced to discharge on the nearby PANI electrode, enabling measuring of MET current. In such a way, the DET and MET currents are measured and the average currents from each bacterium are quantified. As-reported method enables successful disentangling the EET current and may offer valuable insights to related research.

Suggested Citation

  • Shiwu Qian & Chenyu Wang & Changxiang Fang & Meng Li & Yan Qiao & Weihua Hu, 2025. "Disentangling extracellular current of electroactive bacteria with oblique-incidence reflection difference imaging," Nature Communications, Nature, vol. 16(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-62467-6
    DOI: 10.1038/s41467-025-62467-6
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-025-62467-6
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-025-62467-6?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-62467-6. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.