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Quantitative evaluation of methods to analyze motion changes in single-particle experiments

Author

Listed:
  • Gorka Muñoz-Gil

    (University of Innsbruck)

  • Harshith Bachimanchi

    (University of Gothenburg)

  • Jesús Pineda

    (University of Gothenburg)

  • Benjamin Midtvedt

    (University of Gothenburg)

  • Gabriel Fernández-Fernández

    (The Barcelona Institute of Science and Technology)

  • Borja Requena

    (The Barcelona Institute of Science and Technology)

  • Yusef Ahsini

    (Universitat Politècnica de València)

  • Solomon Asghar

    (University College London)

  • Jaeyong Bae

    (Korea Advanced Institute of Science and Technology)

  • Francisco J. Barrantes

    (BIOMED UCA-CONICET)

  • Steen W. B. Bender

    (University of Copenhagen
    University of Copenhagen)

  • Clément Cabriel

    (Université PSL, CNRS)

  • J. Alberto Conejero

    (Universitat Politècnica de València)

  • Marc Escoto

    (Universitat Politècnica de València)

  • Xiaochen Feng

    (Harbin Institute of Technology (Shenzhen))

  • Rasched Haidari

    (University of Oxford
    University of Oxford)

  • Nikos S. Hatzakis

    (University of Copenhagen
    University of Copenhagen)

  • Zihan Huang

    (Hunan University)

  • Ignacio Izeddin

    (Université PSL, CNRS)

  • Hawoong Jeong

    (Korea Advanced Institute of Science and Technology
    Korea Advanced Institute of Science and Technology)

  • Yuan Jiang

    (Harbin Institute of Technology (Shenzhen))

  • Jacob Kæstel-Hansen

    (University of Copenhagen
    University of Copenhagen)

  • Judith Miné-Hattab

    (Sorbonne Université, CNRS)

  • Ran Ni

    (Nanyang Technological University)

  • Junwoo Park

    (Sorbonne Université, CNRS)

  • Xiang Qu

    (Hunan University)

  • Lucas A. Saavedra

    (BIOMED UCA-CONICET)

  • Hao Sha

    (Harbin Institute of Technology (Shenzhen))

  • Nataliya Sokolovska

    (Sorbonne Université, CNRS)

  • Yongbing Zhang

    (Harbin Institute of Technology (Shenzhen))

  • Giorgio Volpe

    (University College London)

  • Maciej Lewenstein

    (The Barcelona Institute of Science and Technology
    ICREA)

  • Ralf Metzler

    (University of Potsdam
    Asia Pacific Centre for Theoretical Physics)

  • Diego Krapf

    (Colorado State University)

  • Giovanni Volpe

    (University of Gothenburg
    University of Gothenburg)

  • Carlo Manzo

    (Universitat de Vic—Universitat Central de Catalunya (UVic-UCC)
    Institut de Recerca i Innovació en Ciències de la Vida i de la Salut a la Catalunya Central (IRIS-CC))

Abstract

The analysis of live-cell single-molecule imaging experiments can reveal valuable information about the heterogeneity of transport processes and interactions between cell components. These characteristics are seen as motion changes in the particle trajectories. Despite the existence of multiple approaches to carry out this type of analysis, no objective assessment of these methods has been performed so far. Here, we report the results of a competition to characterize and rank the performance of these methods when analyzing the dynamic behavior of single molecules. To run this competition, we implemented a software library that simulates realistic data corresponding to widespread diffusion and interaction models, both in the form of trajectories and videos obtained in typical experimental conditions. The competition constitutes the first assessment of these methods, providing insights into the current limitations of the field, fostering the development of new approaches, and guiding researchers to identify optimal tools for analyzing their experiments.

Suggested Citation

  • Gorka Muñoz-Gil & Harshith Bachimanchi & Jesús Pineda & Benjamin Midtvedt & Gabriel Fernández-Fernández & Borja Requena & Yusef Ahsini & Solomon Asghar & Jaeyong Bae & Francisco J. Barrantes & Steen W, 2025. "Quantitative evaluation of methods to analyze motion changes in single-particle experiments," Nature Communications, Nature, vol. 16(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-61949-x
    DOI: 10.1038/s41467-025-61949-x
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