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Stomata-targeted nanocarriers enhance plant defense against pathogen colonization

Author

Listed:
  • Suppanat Puangpathumanond

    (National University of Singapore)

  • Heng Li Chee

    (Technology and Research)

  • Cansu Sevencan

    (National University of Singapore)

  • Xin Yang

    (National University of Singapore)

  • On Sun Lau

    (National University of Singapore
    National University of Singapore)

  • Tedrick Thomas Salim Lew

    (National University of Singapore
    National University of Singapore
    NUS Environmental Research Institute)

Abstract

Plant pathogens significantly threaten food security and agricultural sustainability, with climate change expected to exacerbate outbreaks. Despite these growing threats, current agrochemical delivery remains untargeted and inefficient. In this study, we develop surface ligand-engineered nanoparticles for targeted delivery to stomata (SENDS), a nanocarrier system designed to target stomatal guard cells, which serve as key pathogen entry points into the plant apoplast. Our approach employs rational ligand engineering of porous nanoparticles, optimizing ligand orientation for efficient stomata targeting across different plant species. Foliar application of SENDS encapsulating an antimicrobial plant alkaloid reduces colonization of Xanthomonas campestris, a major crop pathogen, by 20-fold compared to untargeted nanocarriers. Quantitative assessment of stomatal aperture movement and photosynthetic performance confirms that SENDS enhance plant defense against invading pathogens without disrupting natural stomatal function. This nanobiotechnology approach provides a targeted strategy to improve plant disease resistance, offering new insights into nanocarrier design for more resilient and sustainable agriculture.

Suggested Citation

  • Suppanat Puangpathumanond & Heng Li Chee & Cansu Sevencan & Xin Yang & On Sun Lau & Tedrick Thomas Salim Lew, 2025. "Stomata-targeted nanocarriers enhance plant defense against pathogen colonization," Nature Communications, Nature, vol. 16(1), pages 1-16, December.
  • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-60112-w
    DOI: 10.1038/s41467-025-60112-w
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    1. Yuanwei Gou & Dongfang Li & Minghui Zhao & Mengxin Li & Jiaojiao Zhang & Yilian Zhou & Feng Xiao & Gaofei Liu & Haote Ding & Chenfan Sun & Cuifang Ye & Chang Dong & Jucan Gao & Di Gao & Zehua Bao & Le, 2024. "Intein-mediated temperature control for complete biosynthesis of sanguinarine and its halogenated derivatives in yeast," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    2. Jie Tang & Xiaojing Tong & Yongjun Chen & Yue Wu & Zhiyuan Zheng & A. Basak Kayitmazer & Ayyaz Ahmad & Naveed Ramzan & Jintao Yang & Qingchun Huang & Yisheng Xu, 2023. "Deposition and water repelling of temperature-responsive nanopesticides on leaves," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    3. Thomas M. Chaloner & Sarah J. Gurr & Daniel P. Bebber, 2021. "Plant pathogen infection risk tracks global crop yields under climate change," Nature Climate Change, Nature, vol. 11(8), pages 710-715, August.
    4. Manuel Delgado-Baquerizo & Carlos A. Guerra & Concha Cano-Díaz & Eleonora Egidi & Jun-Tao Wang & Nico Eisenhauer & Brajesh K. Singh & Fernando T. Maestre, 2020. "The proportion of soil-borne pathogens increases with warming at the global scale," Nature Climate Change, Nature, vol. 10(6), pages 550-554, June.
    5. Wen Shi & Yue Liu & Na Zhao & Lianmei Yao & Jinge Li & Min Fan & Bojian Zhong & Ming-Yi Bai & Chao Han, 2024. "Hydrogen peroxide is required for light-induced stomatal opening across different plant species," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    6. Joan Dudney & Claire E. Willing & Adrian J. Das & Andrew M. Latimer & Jonathan C. B. Nesmith & John J. Battles, 2021. "Nonlinear shifts in infectious rust disease due to climate change," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    7. Sandeep Sharma & Thomas M. Perring & Su-Ji Jeon & Huazhang Huang & Wen Xu & Emir Islamovic & Bhaskar Sharma & Ysabel Milton Giraldo & Juan Pablo Giraldo, 2024. "Nanocarrier mediated delivery of insecticides into tarsi enhances stink bug mortality," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    8. Joan Dudney & Claire E. Willing & Adrian J. Das & Andrew M. Latimer & Jonathan C. B. Nesmith & John J. Battles, 2021. "Author Correction: Nonlinear shifts in infectious rust disease due to climate change," Nature Communications, Nature, vol. 12(1), pages 1-1, December.
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