IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-27227-2.html
   My bibliography  Save this article

CRISPR-Cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes

Author

Listed:
  • Charlotte Douglas

    (Sex Chromosome Biology Laboratory, The Francis Crick Institute)

  • Valdone Maciulyte

    (Sex Chromosome Biology Laboratory, The Francis Crick Institute)

  • Jasmin Zohren

    (Sex Chromosome Biology Laboratory, The Francis Crick Institute)

  • Daniel M. Snell

    (Sex Chromosome Biology Laboratory, The Francis Crick Institute)

  • Shantha K. Mahadevaiah

    (Sex Chromosome Biology Laboratory, The Francis Crick Institute)

  • Obah A. Ojarikre

    (Sex Chromosome Biology Laboratory, The Francis Crick Institute)

  • Peter J. I. Ellis

    (University of Kent)

  • James M. A. Turner

    (Sex Chromosome Biology Laboratory, The Francis Crick Institute)

Abstract

Animals are essential genetic tools in scientific research and global resources in agriculture. In both arenas, a single sex is often required in surplus. The ethical and financial burden of producing and culling animals of the undesired sex is considerable. Using the mouse as a model, we develop a synthetic lethal, bicomponent CRISPR-Cas9 strategy that produces male- or female-only litters with one hundred percent efficiency. Strikingly, we observe a degree of litter size compensation relative to control matings, indicating that our system has the potential to increase the yield of the desired sex in comparison to standard breeding designs. The bicomponent system can also be repurposed to generate postnatal sex-specific phenotypes. Our approach, harnessing the technological applications of CRISPR-Cas9, may be applicable to other vertebrate species, and provides strides towards ethical improvements for laboratory research and agriculture.

Suggested Citation

  • Charlotte Douglas & Valdone Maciulyte & Jasmin Zohren & Daniel M. Snell & Shantha K. Mahadevaiah & Obah A. Ojarikre & Peter J. I. Ellis & James M. A. Turner, 2021. "CRISPR-Cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27227-2
    DOI: 10.1038/s41467-021-27227-2
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-27227-2
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-27227-2?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Janine A. Clayton & Francis S. Collins, 2014. "Policy: NIH to balance sex in cell and animal studies," Nature, Nature, vol. 509(7500), pages 282-283, May.
    2. Danilo Maddalo & Eusebio Manchado & Carla P. Concepcion & Ciro Bonetti & Joana A. Vidigal & Yoon-Chi Han & Paul Ogrodowski & Alessandra Crippa & Natasha Rekhtman & Elisa de Stanchina & Scott W. Lowe &, 2014. "In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system," Nature, Nature, vol. 516(7531), pages 423-427, December.
    3. Hannah A. Grunwald & Valentino M. Gantz & Gunnar Poplawski & Xiang-Ru S. Xu & Ethan Bier & Kimberly L. Cooper, 2019. "Super-Mendelian inheritance mediated by CRISPR–Cas9 in the female mouse germline," Nature, Nature, vol. 566(7742), pages 105-109, February.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Alena L. Bishop & Víctor López Del Amo & Emily M. Okamoto & Zsolt Bodai & Alexis C. Komor & Valentino M. Gantz, 2022. "Double-tap gene drive uses iterative genome targeting to help overcome resistance alleles," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    2. Alejo Menchaca, 2021. "Sustainable Food Production: The Contribution of Genome Editing in Livestock," Sustainability, MDPI, vol. 13(12), pages 1-16, June.
    3. Andrew T Marshall & Angela T Liu & Niall P Murphy & Nigel T Maidment & Sean B Ostlund, 2017. "Sex-specific enhancement of palatability-driven feeding in adolescent rats," PLOS ONE, Public Library of Science, vol. 12(7), pages 1-23, July.
    4. Sebald A. N. Verkuijl & Estela Gonzalez & Ming Li & Joshua X. D. Ang & Nikolay P. Kandul & Michelle A. E. Anderson & Omar S. Akbari & Michael B. Bonsall & Luke Alphey, 2022. "A CRISPR endonuclease gene drive reveals distinct mechanisms of inheritance bias," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    5. Goulas, Sofoklis & Griselda, Silvia & Megalokonomou, Rigissa, 2020. "Comparative Advantage and Gender Gap in STEM," IZA Discussion Papers 13313, Institute of Labor Economics (IZA).
    6. Sarah Bach & Melissa M Morrow & Kristin D Zhao & Richard E Hughes, 2015. "Sex Distribution of Study Samples Reported in American Society of Biomechanics Annual Meeting Abstracts," PLOS ONE, Public Library of Science, vol. 10(3), pages 1-5, March.
    7. Marek A. Motyka & Ahmed Al-Imam & Aneta Haligowska & Michał Michalak, 2022. "Helping Women Suffering from Drug Addiction: Needs, Barriers, and Challenges," IJERPH, MDPI, vol. 19(21), pages 1-13, October.
    8. Takuji Usui & Malcolm R Macleod & Sarah K McCann & Alistair M Senior & Shinichi Nakagawa, 2021. "Meta-analysis of variation suggests that embracing variability improves both replicability and generalizability in preclinical research," PLOS Biology, Public Library of Science, vol. 19(5), pages 1-20, May.
    9. Lori van den Hurk & Sarah Hiltner & Sabine Oertelt-Prigione, 2022. "Operationalization and Reporting Practices in Manuscripts Addressing Gender Differences in Biomedical Research: A Cross-Sectional Bibliographical Study," IJERPH, MDPI, vol. 19(21), pages 1-13, November.
    10. Laura A. B. Wilson & Susanne R. K. Zajitschek & Malgorzata Lagisz & Jeremy Mason & Hamed Haselimashhadi & Shinichi Nakagawa, 2022. "Sex differences in allometry for phenotypic traits in mice indicate that females are not scaled males," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    11. Noriko Itoh & Yuichiro Itoh & Cassandra E. Meyer & Timothy Takazo Suen & Diego Cortez-Delgado & Michelle Rivera Lomeli & Sophia Wendin & Sri Sanjana Somepalli & Lisa C. Golden & Allan MacKenzie-Graham, 2023. "Estrogen receptor beta in astrocytes modulates cognitive function in mid-age female mice," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    12. Ryan J. Geusz & Allen Wang & Dieter K. Lam & Nicholas K. Vinckier & Konstantinos-Dionysios Alysandratos & David A. Roberts & Jinzhao Wang & Samy Kefalopoulou & Araceli Ramirez & Yunjiang Qiu & Joshua , 2021. "Sequence logic at enhancers governs a dual mechanism of endodermal organ fate induction by FOXA pioneer factors," Nature Communications, Nature, vol. 12(1), pages 1-19, December.
    13. Ava P. Amini & Jesse D. Kirkpatrick & Cathy S. Wang & Alex M. Jaeger & Susan Su & Santiago Naranjo & Qian Zhong & Christina M. Cabana & Tyler Jacks & Sangeeta N. Bhatia, 2022. "Multiscale profiling of protease activity in cancer," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    14. Sara Sanz Juste & Emily M. Okamoto & Christina Nguyen & Xuechun Feng & Víctor López Del Amo, 2023. "Next-generation CRISPR gene-drive systems using Cas12a nuclease," Nature Communications, Nature, vol. 14(1), pages 1-8, December.
    15. Angela Meccariello & Shibo Hou & Serafima Davydova & James Daniel Fawcett & Alexandra Siddall & Philip T. Leftwich & Flavia Krsticevic & Philippos Aris Papathanos & Nikolai Windbichler, 2024. "Gene drive and genetic sex conversion in the global agricultural pest Ceratitis capitata," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    16. April Schweinhart & Janine Austin Clayton, 2018. "Reversing the Trends toward Shorter Lives and Poorer Health for U.S. Women: A Call for Innovative Interdisciplinary Research," IJERPH, MDPI, vol. 15(9), pages 1-14, August.
    17. Ashwin V. Kammula & Alejandro A. Schäffer & Padma Sheila Rajagopal & Razelle Kurzrock & Eytan Ruppin, 2024. "Outcome differences by sex in oncology clinical trials," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    18. Jiang, Xuan, 2021. "Women in STEM: Ability, preference, and value," Labour Economics, Elsevier, vol. 70(C).
    19. Jiang, Xuan, 2018. "Planting the Seeds for Success: Why Women in STEM Do Not Stick in the Field," MPRA Paper 89650, University Library of Munich, Germany.
    20. Michelle A. E. Anderson & Estela Gonzalez & Matthew P. Edgington & Joshua X. D. Ang & Deepak-Kumar Purusothaman & Lewis Shackleford & Katherine Nevard & Sebald A. N. Verkuijl & Timothy Harvey-Samuel &, 2024. "A multiplexed, confinable CRISPR/Cas9 gene drive can propagate in caged Aedes aegypti populations," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27227-2. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.