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Effect of Hydraulic Retention Time on Nutrient Removal in a Microalgae-Based Tertiary Treatment: A Pilot-Scale Study in Winter Conditions

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  • Sofia Vaz

    (A4F—Algae for Future, Campus do Lumiar, Edifício E-R/C, Estrada do Paço do Lumiar, 1648-038 Lisboa, Portugal
    University of Coimbra, CERES—Chemical Engineering and Renewable Resources for Sustainability, Department of Chemical Engineering FCTUC, Rua Sílvio Lima, Pólo II, 3030-790 Coimbra, Portugal)

  • Rui Martins

    (University of Coimbra, CERES—Chemical Engineering and Renewable Resources for Sustainability, Department of Chemical Engineering FCTUC, Rua Sílvio Lima, Pólo II, 3030-790 Coimbra, Portugal)

  • Helena M. Pinheiro

    (iBB—Institute for Bioengineering and Biosciences, Associate Laboratory i4HB—Institute for Health and Bioeconomy, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal)

  • Laura Monteiro

    (A4F—Algae for Future, Campus do Lumiar, Edifício E-R/C, Estrada do Paço do Lumiar, 1648-038 Lisboa, Portugal)

Abstract

The wastewater treatment (WWT) industry is currently facing challenges imposed by the revised urban WWT directive, particularly in terms of nitrogen (N) and phosphorus (P) removal. This implies the need for mandatory tertiary treatment, for which microalgae cultivation shows great sustainability promise. This study investigated the impact of hydraulic retention time (HRT) on nutrient removal in open-air microalgae cultivation for tertiary WWT under winter conditions. Two pilot-scale semi-continuous raceway systems were operated with indigenous microalgae, natural sunlight, and no pH control. HRT values of 4, 5.5, and 7 days were tested, and N, P, and carbon (C) removal and recovery were measured. All conditions allowed nitrogen removal, complying with the revised urban WWT directive. Regarding P, only the 7-day HRT condition consistently complied with the directive’s lowest limit (<0.5 mg P·L −1 ) in the treated water, while 5.5 and 4 days left up to 0.7 and 1.0 mg P·L −1 , respectively, in up to 25% of the samples. A stable microalgae consortium was established under variable light, pH, and dissolved oxygen conditions, albeit with variable biomass productivity. Elemental mass balances revealed that nutrients were mostly recovered in the produced biomass, particularly at high HRT, including effective CO 2 capture from the atmosphere.

Suggested Citation

  • Sofia Vaz & Rui Martins & Helena M. Pinheiro & Laura Monteiro, 2025. "Effect of Hydraulic Retention Time on Nutrient Removal in a Microalgae-Based Tertiary Treatment: A Pilot-Scale Study in Winter Conditions," Sustainability, MDPI, vol. 17(16), pages 1-20, August.
    • Handle: RePEc:gam:jsusta:v:17:y:2025:i:16:p:7553-:d:1729468
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    References listed on IDEAS

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