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Start-Up Strategies for Thermophilic Semi-Continuous Anaerobic Digesters: Assessing the Impact of Inoculum Source and Feed Variability on Efficient Waste-to-Energy Conversion

Author

Listed:
  • Amal Hmaissia

    (BioEngine Research Team on Green Process Engineering and Biorefineries, Chemical Engineering Department, Université Laval, Pavillon Adrien-Pouliot 1065, av. de la Médecine, Québec, QC G1V 0A6, Canada)

  • Edgar Martín Hernández

    (BioEngine Research Team on Green Process Engineering and Biorefineries, Chemical Engineering Department, Université Laval, Pavillon Adrien-Pouliot 1065, av. de la Médecine, Québec, QC G1V 0A6, Canada)

  • Steve Boivin

    (Centre de Biométhanisation de L’agglomération de Québec, Ville de Québec, 100 Chemin de la Baie de Beauport, Québec, QC G1N 4C3, Canada)

  • Céline Vaneeckhaute

    (BioEngine Research Team on Green Process Engineering and Biorefineries, Chemical Engineering Department, Université Laval, Pavillon Adrien-Pouliot 1065, av. de la Médecine, Québec, QC G1V 0A6, Canada)

Abstract

Anaerobic digestion (AD) has gained broad interest as a sustainable organic waste management and resource recovery method. However, the complexity of the AD process could pose serious risks in real-scale applications. One of the most critical phases in the operation of AD systems is the start-up phase, including the seeding strategy of the digesters. This study aims to assess the effect of digestate post-treatment before seeding on the start-up of thermophilic AD systems. Two anaerobic digesters (R1 and R2) were started using two different thermophilic inocula and were kept operational for 17 weeks under identical conditions. Lab digesters were seeded with digestates sampled from a thermophilic full-scale reactor (R2) and a post-treatment mesophilic tank (R1). The start-up strategies exhibited satisfactory stability and high productivity, achieving mean weekly methane-based biodegradability rates of 61 and 64% of the feed’s theoretical biomethane potential (BMP), respectively, in R1 and R2. However, R2 showed greater resilience to high and sudden organic loads applications, making it more suitable for rapid and aggressive start-ups. These results are expected to assist full-scale anaerobic digester operators in selecting an appropriate inoculum based on the characteristics of its source.

Suggested Citation

  • Amal Hmaissia & Edgar Martín Hernández & Steve Boivin & Céline Vaneeckhaute, 2025. "Start-Up Strategies for Thermophilic Semi-Continuous Anaerobic Digesters: Assessing the Impact of Inoculum Source and Feed Variability on Efficient Waste-to-Energy Conversion," Sustainability, MDPI, vol. 17(11), pages 1-22, May.
    • Handle: RePEc:gam:jsusta:v:17:y:2025:i:11:p:5020-:d:1668279
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    References listed on IDEAS

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    1. Jain, Siddharth & Jain, Shivani & Wolf, Ingo Tim & Lee, Jonathan & Tong, Yen Wah, 2015. "A comprehensive review on operating parameters and different pretreatment methodologies for anaerobic digestion of municipal solid waste," Renewable and Sustainable Energy Reviews, Elsevier, vol. 52(C), pages 142-154.
    2. Calbry-Muzyka, Adelaide & Madi, Hossein & Rüsch-Pfund, Florian & Gandiglio, Marta & Biollaz, Serge, 2022. "Biogas composition from agricultural sources and organic fraction of municipal solid waste," Renewable Energy, Elsevier, vol. 181(C), pages 1000-1007.
    3. Chandra, R. & Takeuchi, H. & Hasegawa, T., 2012. "Methane production from lignocellulosic agricultural crop wastes: A review in context to second generation of biofuel production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 16(3), pages 1462-1476.
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