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Effects of Operational Parameters on Biofilm Formation of Mixed Bacteria for Hydrogen Fermentation

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  • Jie Mei

    (Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, Chongqing 400044, China
    Institute of Engineering Thermophysics, School of Energy and Power Engineering, Chongqing University, Chongqing 400044, China)

  • Huize Chen

    (Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, Chongqing 400044, China
    Institute of Engineering Thermophysics, School of Energy and Power Engineering, Chongqing University, Chongqing 400044, China)

  • Qiang Liao

    (Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, Chongqing 400044, China
    Institute of Engineering Thermophysics, School of Energy and Power Engineering, Chongqing University, Chongqing 400044, China)

  • Abdul-Sattar Nizami

    (Sustainable Development Study Centre, Government College University, Lahore 54000, Pakistan)

  • Ao Xia

    (Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, Chongqing 400044, China
    Institute of Engineering Thermophysics, School of Energy and Power Engineering, Chongqing University, Chongqing 400044, China)

  • Yun Huang

    (Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, Chongqing 400044, China
    Institute of Engineering Thermophysics, School of Energy and Power Engineering, Chongqing University, Chongqing 400044, China)

  • Xianqing Zhu

    (Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, Chongqing 400044, China
    Institute of Engineering Thermophysics, School of Energy and Power Engineering, Chongqing University, Chongqing 400044, China)

  • Xun Zhu

    (Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, Chongqing 400044, China
    Institute of Engineering Thermophysics, School of Energy and Power Engineering, Chongqing University, Chongqing 400044, China)

Abstract

Dark fermentation of organic wastes, such as food waste and algae, via mixed hydrogen-producing bacteria (HPB) is considered a sustainable approach for hydrogen production. The biofilm system protects microorganisms from the harmful environment and avoids the excessive loss of bacteria caused by washout, which ensures that the dark fermentation process remains stable. In this study, a downflow anaerobic packed-bed reactor was commissioned to investigate the biofilm formation process of mixed HPB under various operational parameters. Scanning electron microscopy indicated changes in surface morphology during the biofilm formation period. Proteins and polysaccharides in extracellular polymeric substances were identified by confocal laser scanning microscopy to reveal their distribution characteristics. A hydraulic retention time of 0.5 d, a substrate concentration of 15 g/L and an HPB inoculum ratio of 35% were identified as the optimal operational parameters for biofilm formation. The diversity of bacteria between suspension and biofilm showed significantly different distributions; Clostridiales and Lactobacillales were identified as the dominant orders in the biofilm formation process. The abundances of Clostridiales and Lactobacillales were 15.1% and 56.2% in the biofilm, respectively.

Suggested Citation

  • Jie Mei & Huize Chen & Qiang Liao & Abdul-Sattar Nizami & Ao Xia & Yun Huang & Xianqing Zhu & Xun Zhu, 2020. "Effects of Operational Parameters on Biofilm Formation of Mixed Bacteria for Hydrogen Fermentation," Sustainability, MDPI, vol. 12(21), pages 1-15, October.
  • Handle: RePEc:gam:jsusta:v:12:y:2020:i:21:p:8863-:d:434604
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    References listed on IDEAS

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