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Ozonolysis and Enzymatic Pretreatment of Fallen Norway Maple ( Acer platanoides L.) Leaves for Anaerobic Digestion

Author

Listed:
  • Sebastian Gosławski

    (Department of Environmental Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, 90-530 Lodz, Poland)

  • Katarzyna Pielech-Przybylska

    (Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, 90-530 Lodz, Poland)

  • Konrad Jastrząbek

    (Institute of Natural Products and Cosmetics, Faculty of Biotechnology and Food Science, Lodz University of Technology, 90-537 Lodz, Poland)

  • Jarosław Domański

    (Department of Environmental Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, 90-530 Lodz, Poland)

Abstract

Fallen leaves of Norway maple ( Acer platanoides L.) were evaluated as a lignocellulosic substrate for anaerobic digestion (AD). The aim was to identify the pretreatment variant that maximizes methane yield. Leaves were milled and dry sieved, and two particle size classes were selected. The 1–2 cm fraction underwent chemical pretreatment by ozonation only, whereas the 0.5–1 cm fraction was first ozonated and then subjected to biological pretreatment with stepwise dosing of an enzymatic preparation. Ozonation conditions were varied with respect to particle size, ozone concentration (O 3 ), gas flow rate, and contact time. Reducing the particle size from 1–2 cm to 0.5–1 cm increased the cumulative biogas yield by 9.7% (from 64.73 to 71.01 mL g −1 VS) and the methane yield by 13.6% (from 42.01 to 47.75 mL g −1 VS) relative to the control. These findings indicate that particle size reduction combined with ozonation and staged enzymatic dosing is a promising route to improve biomethane recovery from autumn leaf feedstocks.

Suggested Citation

  • Sebastian Gosławski & Katarzyna Pielech-Przybylska & Konrad Jastrząbek & Jarosław Domański, 2025. "Ozonolysis and Enzymatic Pretreatment of Fallen Norway Maple ( Acer platanoides L.) Leaves for Anaerobic Digestion," Energies, MDPI, vol. 18(18), pages 1-22, September.
    • Handle: RePEc:gam:jeners:v:18:y:2025:i:18:p:4963-:d:1752479
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    References listed on IDEAS

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    1. Stanisław Ledakowicz, 2025. "Biochemical Processes of Lignocellulosic Biomass Conversion," Energies, MDPI, vol. 18(13), pages 1-25, June.
    2. Patil, Ravichandra & Cimon, Caroline & Eskicioglu, Cigdem & Goud, Vaibhav, 2021. "Effect of ozonolysis and thermal pre-treatment on rice straw hydrolysis for the enhancement of biomethane production," Renewable Energy, Elsevier, vol. 179(C), pages 467-474.
    3. Lina Luo & Youpei Qu & Weijia Gong & Liyuan Qin & Wenzhe Li & Yong Sun, 2021. "Effect of Particle Size on the Aerobic and Anaerobic Digestion Characteristics of Whole Rice Straw," Energies, MDPI, vol. 14(13), pages 1-15, July.
    4. Agnieszka Wysocka-Czubaszek & Robert Czubaszek, 2024. "Fallen Leaves as a Substrate for Biogas Production," Energies, MDPI, vol. 17(23), pages 1-19, December.
    5. Krystyna Lelicińska-Serafin & Anna Rolewicz-Kalińska & Piotr Manczarski, 2024. "Challenges in the Valorization of Green Waste in the Central European Region: Case Study of Warsaw," Energies, MDPI, vol. 17(20), pages 1-22, October.
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