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Functional Expression of the Arachis hypogaea L. Acyl-ACP Thioesterases AhFatA and AhFatB Enhances Fatty Acid Production in Synechocystis sp. PCC6803

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  • Gao Chen

    (Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Ji’nan 250100, China
    Shandong Provincial Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops, Ji’nan 250100, China
    The authors contributed equally to this work. Jun Chen is the co-first author and has the same contribution to this paper.)

  • Jun Chen

    (Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China
    University of Chinese Academy of Sciences, Beijing 100049, China
    The authors contributed equally to this work. Jun Chen is the co-first author and has the same contribution to this paper.)

  • Qingfang He

    (Department of Biology, University of Arkansas at Little Rock, Little Rock, AR 72204, USA)

  • Yan Zhang

    (Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Ji’nan 250100, China
    Shandong Provincial Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops, Ji’nan 250100, China)

  • Zhenying Peng

    (Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Ji’nan 250100, China
    Shandong Provincial Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops, Ji’nan 250100, China)

  • Zhongxue Fan

    (Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Ji’nan 250100, China
    Shandong Provincial Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops, Ji’nan 250100, China)

  • Fei Bian

    (Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Ji’nan 250100, China
    Shandong Provincial Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops, Ji’nan 250100, China)

  • Jinhui Yu

    (Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Ji’nan 250100, China
    Shandong Provincial Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops, Ji’nan 250100, China)

  • Song Qin

    (University of Chinese Academy of Sciences, Beijing 100049, China)

Abstract

Palmitoleic acid (C16:1) and stearic acid (C18:0) are precursors of polyunsaturated fatty acids, which are the focus of intensive global research due to their nutritional value, medicinal applications, and potential use as biofuel. Acyl-acyl carrier protein (ACP) thioesterases are intraplastidial enzymes that determine the types and amounts of fatty acids produced in plants and release fatty acids into the cytosol to be incorporated into glycerolipids. Based on amino acid sequence identity and substrate specificity, these enzymes are classified into two families, FatA and FatB . In this study, we cloned FatA and FatB thioesterases from Arachis hypogaea L. seeds and functionally expressed these genes, both individually and in tandem, in a blue-green alga Synechocystis sp. PCC6803. The heterologous expression of these genes in Synechocystis altered the fatty acid composition of lipids, resulting in a 29.5–31.6% increase in palmitoleic acid production and a 22.5–35.5% increase in stearic acid production. Moreover, the transgenic Synechocystis cells also showed significant increases in levels of oleic acid (C18:1, OA), linoleic acid (C18:2, LA), and α-linolenic acid (C18:3n3, ALA). These results suggest that the fatty acid profile of algae can be significantly improved by the heterologous expression of exogenous genes. This study not only provides insight into fatty acid biosynthesis, but also lays the foundation for manipulating the fatty acid content of cyanobacteria.

Suggested Citation

  • Gao Chen & Jun Chen & Qingfang He & Yan Zhang & Zhenying Peng & Zhongxue Fan & Fei Bian & Jinhui Yu & Song Qin, 2017. "Functional Expression of the Arachis hypogaea L. Acyl-ACP Thioesterases AhFatA and AhFatB Enhances Fatty Acid Production in Synechocystis sp. PCC6803," Energies, MDPI, vol. 10(12), pages 1-12, December.
    • Handle: RePEc:gam:jeners:v:10:y:2017:i:12:p:2093-:d:122319
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    References listed on IDEAS

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    1. Brennan, Liam & Owende, Philip, 2010. "Biofuels from microalgae--A review of technologies for production, processing, and extractions of biofuels and co-products," Renewable and Sustainable Energy Reviews, Elsevier, vol. 14(2), pages 557-577, February.
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    1. Jerónimo Chirivella-Martorell & Álvaro Briz-Redón & Ángel Serrano-Aroca, 2018. "Modelling of Biomass Concentration, Multi-Wavelength Absorption and Discrimination Method for Seven Important Marine Microalgae Species," Energies, MDPI, vol. 11(5), pages 1-13, April.

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