IDEAS home Printed from https://ideas.repec.org/a/epw/ejbio0/v3y2022i2id17332.html

CRISPR-Cas Technology, the Tool of the Future

Author

Listed:
  • Alejandro Esquivel Álvarez
  • Elías Quesada Morales
  • María Paz Villegas Calero
  • Daniela Matarrita Brenes
  • María Fernanda Rojas Salas
  • German Madrigal Redondo
  • Marianela Chavarría Rojas
  • Eleaneth Baltodano Viales

Abstract

The CRISPR-Cas system discovered in the eighties consists of a series of RNA proteins that signal and messengers of the immune system in prokaryotic organisms to protect against invading antigens of various sources. After the CRISPR-Cas discovery, many research advances were made in the following decades regarding knowledge, techniques, and applications. This showed the system could edit the DNA in an exact and specific manner, which made it very promising to exploit it in various fields, such as the therapeutic field. New therapies of various diseases, industrial applications, food manufacturing, among others, make its impact quite relevant. The following review will focus on the fundamental understanding and implications of CRISPR-Cas techniques has with an ethical and legal view. In addition, to explore some of the applications present and future in the healthcare department, some methods of drug delivery in gene therapy, and new research that is being developed with the CRISPR-Cas technology.

Suggested Citation

  • Alejandro Esquivel Álvarez & Elías Quesada Morales & María Paz Villegas Calero & Daniela Matarrita Brenes & María Fernanda Rojas Salas & German Madrigal Redondo & Marianela Chavarría Rojas & Eleaneth , 2022. "CRISPR-Cas Technology, the Tool of the Future," European Journal of Biology and Biotechnology, European Open Science, vol. 3(2), pages 1-9, March.
  • Handle: RePEc:epw:ejbio0:v:3:y:2022:i:2:id:17332
    DOI: 10.24018/ejbio.2022.3.2.332
    as

    Download full text from publisher

    File URL: https://eu-opensci.org/index.php/ejbio/article/view/17332
    File Function: Abstract page
    Download Restriction: no

    File URL: https://eu-opensci.org/index.php/ejbio/article/download/17332/4247
    File Function: Full text
    Download Restriction: no

    File URL: https://libkey.io/10.24018/ejbio.2022.3.2.332?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Serif Senturk & Nitin H. Shirole & Dawid G. Nowak & Vincenzo Corbo & Debjani Pal & Alexander Vaughan & David A. Tuveson & Lloyd C. Trotman & Justin B. Kinney & Raffaella Sordella, 2017. "Rapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization," Nature Communications, Nature, vol. 8(1), pages 1-10, April.
    2. Shanmugam, Sabarathinam & Ngo, Huu-Hao & Wu, Yi-Rui, 2020. "Advanced CRISPR/Cas-based genome editing tools for microbial biofuels production: A review," Renewable Energy, Elsevier, vol. 149(C), pages 1107-1119.
    3. Seth L. Shipman & Jeff Nivala & Jeffrey D. Macklis & George M. Church, 2017. "CRISPR–Cas encoding of a digital movie into the genomes of a population of living bacteria," Nature, Nature, vol. 547(7663), pages 345-349, July.
    4. Wen Xue & Sidi Chen & Hao Yin & Tuomas Tammela & Thales Papagiannakopoulos & Nikhil S. Joshi & Wenxin Cai & Gillian Yang & Roderick Bronson & Denise G. Crowley & Feng Zhang & Daniel G. Anderson & Phil, 2014. "CRISPR-mediated direct mutation of cancer genes in the mouse liver," Nature, Nature, vol. 514(7522), pages 380-384, October.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Xing Cheng & Jing An & Jitong Lou & Qisheng Gu & Weimin Ding & Gaith Nabil Droby & Yilin Wang & Chenghao Wang & Yanzhe Gao & Jay Ramanlal Anand & Abigail Shelton & Andrew Benson Satterlee & Breanna Ma, 2024. "Trans-lesion synthesis and mismatch repair pathway crosstalk defines chemoresistance and hypermutation mechanisms in glioblastoma," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
    2. Abdur Rawoof, Salma Aathika & Kumar, P. Senthil & Vo, Dai-Viet N. & Devaraj, Thiruselvi & Subramanian, Sivanesan, 2021. "Biohythane as a high potential fuel from anaerobic digestion of organic waste: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 152(C).
    3. Sabarathinam Shanmugam & Anjana Hari & Arivalagan Pugazhendhi & Timo Kikas, 2023. "Integrated Catalytic Upgrading of Biomass-Derived Alcohols for Advanced Biofuel Production," Energies, MDPI, vol. 16(13), pages 1-24, June.
    4. Faran Mustafa & Abu Al Hussain & M Zargham Ali & Shaheera Qureshi & Asma Arif & Hafiz Muhammad Zia Ullah Ghazali & Muhammad Sajjad, 2021. "Influence of Nanoparticles on the Growth, Development and Germination of Plants and Their Subsequent Effects on the Environment - A Review," Biomedical Journal of Scientific & Technical Research, Biomedical Research Network+, LLC, vol. 40(2), pages 32098-32112, November.
    5. Mamata Singhvi & Smita Zinjarde & Beom-Soo Kim, 2022. "Sustainable Strategies for the Conversion of Lignocellulosic Materials into Biohydrogen: Challenges and Solutions toward Carbon Neutrality," Energies, MDPI, vol. 15(23), pages 1-13, November.
    6. Cheng Kai Lim & Jing Wui Yeoh & Aurelius Andrew Kunartama & Wen Shan Yew & Chueh Loo Poh, 2023. "A biological camera that captures and stores images directly into DNA," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    7. Christel F. A. Ramirez & Daniel Taranto & Masami Ando-Kuri & Marnix H. P. Groot & Efi Tsouri & Zhijie Huang & Daniel Groot & Roelof J. C. Kluin & Daan J. Kloosterman & Joanne Verheij & Jing Xu & Seren, 2024. "Cancer cell genetics shaping of the tumor microenvironment reveals myeloid cell-centric exploitable vulnerabilities in hepatocellular carcinoma," Nature Communications, Nature, vol. 15(1), pages 1-24, December.
    8. Yazhong Cui & Yang Miao & Longzhi Cao & Lifang Guo & Yue Cui & Chuanzhe Yan & Zhi Zeng & Mo Xu & Ting Han, 2023. "Activation of melanocortin-1 receptor signaling in melanoma cells impairs T cell infiltration to dampen antitumor immunity," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    9. Basheer Becerra & Sandra Wittibschlager & Zain M. Patel & Ana P. Kutschat & Justin Delano & Eric Che & Anzhelika Tauber & Ting Wu & Marlena Starrs & Christina S. Horstmann & Sophie Müller & Madelynn N, 2026. "Nucleotide-resolution mapping of regulatory elements via allelic readout of tiled base editing," Nature Communications, Nature, vol. 17(1), pages 1-16, December.
    10. Arpiar Saunders & Kee Wui Huang & Cassandra Vondrak & Christina Hughes & Karina Smolyar & Harsha Sen & Adrienne C. Philson & James Nemesh & Alec Wysoker & Seva Kashin & Bernardo L. Sabatini & Steven A, 2022. "Ascertaining cells’ synaptic connections and RNA expression simultaneously with barcoded rabies virus libraries," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    11. Tamás Mizik & Christian Barika Igbeghe & Zsuzsanna Deák, 2025. "Production Efficiency of Advanced Liquid Biofuels: Prospects and Challenges," Energies, MDPI, vol. 18(4), pages 1-18, February.
    12. Rajini Srinivasan & Tao Sun & Azalia Sandles & Diana Wu & Liang Wang & Hetal Patel & Ryan Pabalate & Maram Bader & Amy Heidersbach & Clark Ho & Shiqi Xie & Andrew Ng & Benjamin Haley, 2026. "Chemically-inducible CRISPR/Cas9 circuits for ultra-high dynamic range gene perturbation," Nature Communications, Nature, vol. 17(1), pages 1-16, December.
    13. Dixit, Yatika & Yadav, Preeti & Sharma, Arun Kumar & Pandey, Poornima & Kuila, Arindam, 2023. "Multiplex genome editing to construct cellulase engineered Saccharomyces cerevisiae for ethanol production from cellulosic biomass," Renewable and Sustainable Energy Reviews, Elsevier, vol. 187(C).
    14. Duško Lainšček & Vida Forstnerič & Veronika Mikolič & Špela Malenšek & Peter Pečan & Mojca Benčina & Matjaž Sever & Helena Podgornik & Roman Jerala, 2022. "Coiled-coil heterodimer-based recruitment of an exonuclease to CRISPR/Cas for enhanced gene editing," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    15. Swathi Somaiyan Babu & Rashmi Gondi & Godvin Sharmila Vincent & Godwin Christopher JohnSamuel & Rajesh Banu Jeyakumar, 2022. "Microalgae Biomass and Lipids as Feedstock for Biofuels: Sustainable Biotechnology Strategies," Sustainability, MDPI, vol. 14(22), pages 1-31, November.
    16. Bakioglu, Gozde, 2024. "Selection of sustainable transportation strategies for campuses using hybrid decision-making approach under picture fuzzy sets," Technological Forecasting and Social Change, Elsevier, vol. 206(C).
    17. Afsaneh Sadremomtaz & Robert F. Glass & Jorge Eduardo Guerrero & Dennis R. LaJeunesse & Eric A. Josephs & Reza Zadegan, 2023. "Digital data storage on DNA tape using CRISPR base editors," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    18. Omnia M. Elsharkasy & Charlotte V. Hegeman & Tom A. P. Driedonks & Xiuming Liang & Ivana Lansweers & Olaf L. Cotugno & Ingmar Y. de Groot & Zoë E. M. N. J. de Wit & Antonio Garcia-Guerra & Niels J. A., 2025. "A modular strategy for extracellular vesicle-mediated CRISPR-Cas9 delivery through aptamer-based loading and UV-activated cargo release," Nature Communications, Nature, vol. 16(1), pages 1-18, December.

    More about this item

    Keywords

    ;
    ;
    ;
    ;
    ;
    ;

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:epw:ejbio0:v:3:y:2022:i:2:id:17332. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Support Team (email available below). General contact details of provider: https://eu-opensci.org/index.php/ejbio .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.