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CRISPR-mediated direct mutation of cancer genes in the mouse liver

Author

Listed:
  • Wen Xue

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

  • Sidi Chen

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

  • Hao Yin

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

  • Tuomas Tammela

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

  • Thales Papagiannakopoulos

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

  • Nikhil S. Joshi

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

  • Wenxin Cai

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

  • Gillian Yang

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

  • Roderick Bronson

    (Tufts University and Harvard Medical School)

  • Denise G. Crowley

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

  • Feng Zhang

    (Broad Institute of Massachusetts Institute of Technology and Harvard)

  • Daniel G. Anderson

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
    Massachusetts Institute of Technology
    Harvard-MIT Division of Health Sciences & Technology
    Institute for Medical Engineering and Science, Massachusetts Institute of Technology)

  • Phillip A. Sharp

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
    Massachusetts Institute of Technology)

  • Tyler Jacks

    (David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
    Massachusetts Institute of Technology
    Howard Hughes Medical Institute, Massachusetts Institute of Technology)

Abstract

CRISPR plasmids targeting Pten and p53, alone and in combination, are delivered by hydrodynamic injection to the liver; the CRISPR-mediated mutations phenocopy the effects of deletions using Cre–LoxP technology, allowing the direct mutation of tumour suppressor genes and oncogenes in the liver using the CRISPR/Cas system, which presents a new approach for rapid development of liver cancer models and functional genomics.

Suggested Citation

  • Wen Xue & Sidi Chen & Hao Yin & Tuomas Tammela & Thales Papagiannakopoulos & Nikhil S. Joshi & Wenxin Cai & Gillian Yang & Roderick Bronson & Denise G. Crowley & Feng Zhang & Daniel G. Anderson & Phil, 2014. "CRISPR-mediated direct mutation of cancer genes in the mouse liver," Nature, Nature, vol. 514(7522), pages 380-384, October.
    • Handle: RePEc:nat:nature:v:514:y:2014:i:7522:d:10.1038_nature13589
    DOI: 10.1038/nature13589
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    Cited by:

    1. Christel F. A. Ramirez & Daniel Taranto & Masami Ando-Kuri & Marnix H. P. Groot & Efi Tsouri & Zhijie Huang & Daniel Groot & Roelof J. C. Kluin & Daan J. Kloosterman & Joanne Verheij & Jing Xu & Seren, 2024. "Cancer cell genetics shaping of the tumor microenvironment reveals myeloid cell-centric exploitable vulnerabilities in hepatocellular carcinoma," Nature Communications, Nature, vol. 15(1), pages 1-24, December.
    2. Yazhong Cui & Yang Miao & Longzhi Cao & Lifang Guo & Yue Cui & Chuanzhe Yan & Zhi Zeng & Mo Xu & Ting Han, 2023. "Activation of melanocortin-1 receptor signaling in melanoma cells impairs T cell infiltration to dampen antitumor immunity," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    3. Duško Lainšček & Vida Forstnerič & Veronika Mikolič & Špela Malenšek & Peter Pečan & Mojca Benčina & Matjaž Sever & Helena Podgornik & Roman Jerala, 2022. "Coiled-coil heterodimer-based recruitment of an exonuclease to CRISPR/Cas for enhanced gene editing," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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