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Critical Level of 13C Enrichment for the Successful Isolation of 13C Labeled DNA

Author

Listed:
  • Teng-xiang Lian
  • Guang-hua Wang
  • Zhen-hua Yu
  • Xiao-bing Liu
  • Jian Jin Author-Workplace- Name:Department of Soil Science and Plant Nutrition, Namık Kemal University, Turkey
  • Teng-xiang Lian Author-Workplace- Name: University of Chinese Academy of Sciences, China

Abstract

In the stable-isotope probing (SIP) technology, the 13C-labelled substrate is normally used to incorporate 13C into nucleic acids. A proper abundance of 13C in substrate is critical to the success of SIP, because the 13C level not only determines whether 13C in nucleic acids is sufficient to be detective, but also affects the enrichment bias in the labeled microbes. However, such information is very rare. In this study, a serial of 13C-labelled glucose from 0 to 50 atom% 13C was used to incubate with Escherichia coli and then performed DNA-SIP. Our results showed that the detective level of 13C-DNA could be reduced to 2 atom% 13C of glucose (1.30 atom% 13C in DNA extract), while the ideal level was 10 atom% 13C glucose (2.25 atom% 13C in DNA).

Suggested Citation

  • Teng-xiang Lian & Guang-hua Wang & Zhen-hua Yu & Xiao-bing Liu & Jian Jin Author-Workplace- Name:Department of Soil Science and Plant Nutrition, Namık Kemal University, Turkey & Teng-xiang Lian Autho, 2016. "Critical Level of 13C Enrichment for the Successful Isolation of 13C Labeled DNA," Agricultural Research & Technology: Open Access Journal, Juniper Publishers Inc., vol. 1(2), pages 29-33, January.
    • Handle: RePEc:adp:artoaj:v:1:y:2016:i:2:p:29-33
    DOI: 10.19080/ARTOAJ.2016.01.555558
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    References listed on IDEAS

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    1. Stefan Radajewski & Philip Ineson & Nisha R. Parekh & J. Colin Murrell, 2000. "Stable-isotope probing as a tool in microbial ecology," Nature, Nature, vol. 403(6770), pages 646-649, February.
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