IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0150182.html
   My bibliography  Save this article

Reproducibility of Fluorescent Expression from Engineered Biological Constructs in E. coli

Author

Listed:
  • Jacob Beal
  • Traci Haddock-Angelli
  • Markus Gershater
  • Kim de Mora
  • Meagan Lizarazo
  • Jim Hollenhorst
  • Randy Rettberg
  • iGEM Interlab Study Contributors

Abstract

We present results of the first large-scale interlaboratory study carried out in synthetic biology, as part of the 2014 and 2015 International Genetically Engineered Machine (iGEM) competitions. Participants at 88 institutions around the world measured fluorescence from three engineered constitutive constructs in E. coli. Few participants were able to measure absolute fluorescence, so data was analyzed in terms of ratios. Precision was strongly related to fluorescent strength, ranging from 1.54-fold standard deviation for the ratio between strong promoters to 5.75-fold for the ratio between the strongest and weakest promoter, and while host strain did not affect expression ratios, choice of instrument did. This result shows that high quantitative precision and reproducibility of results is possible, while at the same time indicating areas needing improved laboratory practices.

Suggested Citation

  • Jacob Beal & Traci Haddock-Angelli & Markus Gershater & Kim de Mora & Meagan Lizarazo & Jim Hollenhorst & Randy Rettberg & iGEM Interlab Study Contributors, 2016. "Reproducibility of Fluorescent Expression from Engineered Biological Constructs in E. coli," PLOS ONE, Public Library of Science, vol. 11(3), pages 1-22, March.
    • Handle: RePEc:plo:pone00:0150182
    DOI: 10.1371/journal.pone.0150182
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0150182
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0150182&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0150182?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0150182. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.