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Single-cell transcriptomics of ventral forebrain progenitors identifies Evf2 enhancer lncRNA-enhancer gene guidance through direct RNA binding and RNP recruitment domains

Author

Listed:
  • Edward Li

    (Feinberg School of Medicine)

  • Abhijit Chakraborty

    (La Jolla Institute for Immunology)

  • Sara J. Kohtz

    (Binghamton University)

  • Ivelisse Cajigas

    (Feinberg School of Medicine
    Abbott Labs)

  • Laura Hinojosa-Gonzalez

    (La Jolla Institute for Immunology
    University of California San Diego)

  • Fion Shiau

    (Washington University School of Medicine)

  • Ryan Bertossi

    (Feinberg School of Medicine)

  • Robert J. Vassar

    (Feinberg School of Medicine)

  • Jack A. Kessler

    (Feinberg School of Medicine)

  • Ferhat Ay

    (La Jolla Institute for Immunology
    University of California San Diego
    University of California San Diego)

  • Brian S. Clark

    (Washington University School of Medicine
    Washington University School of Medicine)

  • Jhumku D. Kohtz

    (Feinberg School of Medicine)

Abstract

During mouse embryonic brain development, the Evf2 ultraconserved enhancer (UCE) lncRNA guides the Dlx5/6UCE to ~129 sites across chr6. However, previous work identified only 4 transcriptionally regulated targets associated with Evf2-Dlx5/6UCE-gene guidance, raising questions about the significance of Evf2-regulated Dlx5/6UCE-gene interactions. Here, single-cell transcriptomics reveal far greater alignment between Evf2-Dlx5/6UCE-gene guidance and transcriptional regulation than previously reported. Evf2 divides chr6 into short-range (

Suggested Citation

  • Edward Li & Abhijit Chakraborty & Sara J. Kohtz & Ivelisse Cajigas & Laura Hinojosa-Gonzalez & Fion Shiau & Ryan Bertossi & Robert J. Vassar & Jack A. Kessler & Ferhat Ay & Brian S. Clark & Jhumku D. , 2025. "Single-cell transcriptomics of ventral forebrain progenitors identifies Evf2 enhancer lncRNA-enhancer gene guidance through direct RNA binding and RNP recruitment domains," Nature Communications, Nature, vol. 16(1), pages 1-23, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-62205-y
    DOI: 10.1038/s41467-025-62205-y
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