IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-49093-4.html
   My bibliography  Save this article

Faecal microbiota of schoolchildren is associated with nutritional status and markers of inflammation: a double-blinded cluster-randomized controlled trial using multi-micronutrient fortified rice

Author

Listed:
  • Yohannes Seyoum

    (Université de La Réunion)

  • Valérie Greffeuille

    (Université de La Réunion)

  • Dorgeles Kouakou Dje Kouadio

    (Université de La Réunion)

  • Khov Kuong

    (Ministry of Agriculture, Forestry and Fisheries)

  • Williams Turpin

    (Université de La Réunion
    Mount Sinai Hospital
    University of Toronto)

  • Rachida M’Rabt

    (Université de La Réunion)

  • Vincent Chochois

    (Université de La Réunion)

  • Sonia Fortin

    (Université de La Réunion)

  • Marlène Perignon

    (Université de La Réunion
    IRD)

  • Marion Fiorentino

    (Université de La Réunion
    Aix Marseille Univ)

  • Jacques Berger

    (Université de La Réunion)

  • Kurt Burja

    (United Nations World Food Programme)

  • Maiza Campos Ponce

    (VU University Amsterdam)

  • Chhoun Chamnan

    (Ministry of Agriculture, Forestry and Fisheries)

  • Frank T. Wieringa

    (Université de La Réunion)

  • Christèle Humblot

    (Université de La Réunion)

Abstract

Faecal microbiota plays a critical role in human health, but its relationship with nutritional status among schoolchildren remains under-explored. Here, in a double-blinded cluster-randomized controlled trial on 380 Cambodian schoolchildren, we characterize the impact of six months consumption of two types of rice fortified with different levels of vitamins and minerals on pre-specified outcomes. We investigate the association between the faecal microbiota (16SrRNA sequencing) and age, sex, nutritional status (underweight, stunting), micronutrient status (iron, zinc and vitamin A deficiencies, anaemia, iron deficient anaemia, hemoglobinopathy), inflammation (systemic, gut), and parasitic infection. We show that the faecal microbiota is characterised by a surprisingly high proportion of Lactobacillaceae. We discover that deficiencies in specific micronutrients, such as iron and vitamin A, correlate with particular microbiota profiles, whereas zinc deficiency shows no such association. The nutritional intervention with the two rice treatments impacts both the composition and functions predicted from compositional analysis in different ways. (ClinicalTrials.gov (Identifier: NCT01706419))

Suggested Citation

  • Yohannes Seyoum & Valérie Greffeuille & Dorgeles Kouakou Dje Kouadio & Khov Kuong & Williams Turpin & Rachida M’Rabt & Vincent Chochois & Sonia Fortin & Marlène Perignon & Marion Fiorentino & Jacques , 2024. "Faecal microbiota of schoolchildren is associated with nutritional status and markers of inflammation: a double-blinded cluster-randomized controlled trial using multi-micronutrient fortified rice," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-49093-4
    DOI: 10.1038/s41467-024-49093-4
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-49093-4
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-49093-4?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Himel Mallick & Ali Rahnavard & Lauren J McIver & Siyuan Ma & Yancong Zhang & Long H Nguyen & Timothy L Tickle & George Weingart & Boyu Ren & Emma H Schwager & Suvo Chatterjee & Kelsey N Thompson & Je, 2021. "Multivariable association discovery in population-scale meta-omics studies," PLOS Computational Biology, Public Library of Science, vol. 17(11), pages 1-27, November.
    2. Julien Tap & Franck Lejzerowicz & Aurélie Cotillard & Matthieu Pichaud & Daniel McDonald & Se Jin Song & Rob Knight & Patrick Veiga & Muriel Derrien, 2023. "Global branches and local states of the human gut microbiome define associations with environmental and intrinsic factors," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    3. Sathish Subramanian & Sayeeda Huq & Tanya Yatsunenko & Rashidul Haque & Mustafa Mahfuz & Mohammed A. Alam & Amber Benezra & Joseph DeStefano & Martin F. Meier & Brian D. Muegge & Michael J. Barratt & , 2014. "Persistent gut microbiota immaturity in malnourished Bangladeshi children," Nature, Nature, vol. 510(7505), pages 417-421, June.
    4. Andreas J. Bäumler & Vanessa Sperandio, 2016. "Interactions between the microbiota and pathogenic bacteria in the gut," Nature, Nature, vol. 535(7610), pages 85-93, July.
    5. Peter J. Turnbaugh & Ruth E. Ley & Michael A. Mahowald & Vincent Magrini & Elaine R. Mardis & Jeffrey I. Gordon, 2006. "An obesity-associated gut microbiome with increased capacity for energy harvest," Nature, Nature, vol. 444(7122), pages 1027-1031, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. T. Portlock & T. Shama & S. H. Kakon & B. Hartjen & C. Pook & B. C. Wilson & A. Bhuttor & D. Ho & I. Shennon & A. M. Engelstad & R. Lorenzo & G. Greaves & N. Rahman & C. Kelsey & P. D. Gluckman & J. M, 2025. "Interconnected pathways link faecal microbiota plasma lipids and brain activity to childhood malnutrition related cognition," Nature Communications, Nature, vol. 16(1), pages 1-14, December.
    2. Ruairi C. Robertson & Thaddeus J. Edens & Lynnea Carr & Kuda Mutasa & Ethan K. Gough & Ceri Evans & Hyun Min Geum & Iman Baharmand & Sandeep K. Gill & Robert Ntozini & Laura E. Smith & Bernard Chasekw, 2023. "The gut microbiome and early-life growth in a population with high prevalence of stunting," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    3. Karen D. Corbin & Elvis A. Carnero & Blake Dirks & Daria Igudesman & Fanchao Yi & Andrew Marcus & Taylor L. Davis & Richard E. Pratley & Bruce E. Rittmann & Rosa Krajmalnik-Brown & Steven R. Smith, 2023. "Host-diet-gut microbiome interactions influence human energy balance: a randomized clinical trial," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    4. Hidenori Shimizu & Junki Miyamoto & Keiko Hisa & Ryuji Ohue-Kitano & Hiromi Takada & Mayu Yamano & Akari Nishida & Daiki Sasahara & Yuki Masujima & Keita Watanabe & Shota Nishikawa & Sakura Takahashi , 2025. "Sucrose-preferring gut microbes prevent host obesity by producing exopolysaccharides," Nature Communications, Nature, vol. 16(1), pages 1-16, December.
    5. Kiran Konain & Sadia & Turfa Nadeem & Adeed Khan & Warda Iqbal & Arsalan & Amir Javed & Ruby Khan & Kainat Jamil & Kainat Jamil, 2018. "Importance of Probiotics in Gastrointestinal Tract," Journal of Asian Scientific Research, Asian Economic and Social Society, vol. 8(3), pages 128-143, March.
    6. Shiao-Pei Weathers & Xiqi Li & Haifeng Zhu & Ashish V. Damania & Mark Knafl & Brian McKinley & Heather Lin & Rebecca A. Harrison & Nazanin K. Majd & Barbara J. O’Brien & Marta Penas-Prado & Monica Log, 2025. "Improved overall survival in an anti-PD-L1 treated cohort of newly diagnosed glioblastoma patients is associated with distinct immune, mutation, and gut microbiome features: a single arm prospective p," Nature Communications, Nature, vol. 16(1), pages 1-13, December.
    7. Marjolein Heddes & Baraa Altaha & Yunhui Niu & Sandra Reitmeier & Karin Kleigrewe & Dirk Haller & Silke Kiessling, 2022. "The intestinal clock drives the microbiome to maintain gastrointestinal homeostasis," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    8. Manish Boolchandani & Kevin S. Blake & Drake H. Tilley & Miguel M. Cabada & Drew J. Schwartz & Sanket Patel & Maria Luisa Morales & Rina Meza & Giselle Soto & Sandra D. Isidean & Chad K. Porter & Mark, 2022. "Impact of international travel and diarrhea on gut microbiome and resistome dynamics," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    9. Lishi Deng & Steff Taelman & Matthew R. Olm & Laeticia Celine Toe & Eva Balini & Lionel Olivier Ouédraogo & Yuri Bastos-Moreira & Alemayehu Argaw & Kokeb Tesfamariam & Erica D. Sonnenburg & Giles T. H, 2025. "Maternal balanced energy-protein supplementation reshapes the maternal gut microbiome and enhances carbohydrate metabolism in infants: a randomized controlled trial," Nature Communications, Nature, vol. 16(1), pages 1-16, December.
    10. Natasha Spottiswoode & Alexandra Tsitsiklis & Victoria T. Chu & Hoang Van Phan & Catherine DeVoe & Christina Love & Rajani Ghale & Joshua Bloomstein & Beth Shoshana Zha & Cole P. Maguire & Abigail Gla, 2024. "Microbial dynamics and pulmonary immune responses in COVID-19 secondary bacterial pneumonia," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    11. Curtis Cottam & Rhys T. White & Lauren C. Beck & Christopher J. Stewart & Scott A. Beatson & Elisabeth C. Lowe & Rhys Grinter & James P. R. Connolly, 2024. "Metabolism of l-arabinose converges with virulence regulation to promote enteric pathogen fitness," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    12. repec:plo:pone00:0125889 is not listed on IDEAS
    13. Cristiane R. S. Câmara & Carlos A. Urrea & Vicki Schlegel, 2013. "Pinto Beans ( Phaseolus vulgaris L.) as a Functional Food: Implications on Human Health," Agriculture, MDPI, vol. 3(1), pages 1-22, February.
    14. Fanette Fontaine & Sondra Turjeman & Karel Callens & Omry Koren, 2023. "The intersection of undernutrition, microbiome, and child development in the first years of life," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    15. Vinod Nikhra, 2019. "The Novel Dimensions of Cardio-Metabolic Health Gut Microbiota, Dysbiosis and its Fallouts," Current Research in Diabetes & Obesity Journal, Juniper Publishers Inc., vol. 11(1), pages 28-37, June.
    16. Jacek Piatek & Hanna Krauss & Arleta Ciechelska-Rybarczyk & Malgorzata Bernatek & Paulina Wojtyla-Buciora & Henning Sommermeyer, 2020. "In-Vitro Growth Inhibition of Bacterial Pathogens by Probiotics and a Synbiotic: Product Composition Matters," IJERPH, MDPI, vol. 17(9), pages 1-10, May.
    17. Shinji Fukuda & Yumiko Nakanishi & Eisuke Chikayama & Hiroshi Ohno & Tsuneo Hino & Jun Kikuchi, 2009. "Evaluation and Characterization of Bacterial Metabolic Dynamics with a Novel Profiling Technique, Real-Time Metabolotyping," PLOS ONE, Public Library of Science, vol. 4(3), pages 1-10, March.
    18. James Robert White & Niranjan Nagarajan & Mihai Pop, 2009. "Statistical Methods for Detecting Differentially Abundant Features in Clinical Metagenomic Samples," PLOS Computational Biology, Public Library of Science, vol. 5(4), pages 1-11, April.
    19. repec:plo:pone00:0093631 is not listed on IDEAS
    20. Bo Tang & Li Tang & Shengpeng Li & Shuang Liu & Jialin He & Pan Li & Sumin Wang & Min Yang & Longhui Zhang & Yuanyuan Lei & Dianji Tu & Xuefeng Tang & Hua Hu & Qin Ouyang & Xia Chen & Shiming Yang, 2023. "Gut microbiota alters host bile acid metabolism to contribute to intrahepatic cholestasis of pregnancy," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    21. Lingling Wang & Haobin Yao & Daniel C. Morgan & Kam Shing Lau & Suet Yi Leung & Joshua W. K. Ho & Wai K. Leung, 2023. "Altered human gut virome in patients undergoing antibiotics therapy for Helicobacter pylori," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    22. Michael DiMarzio & Brigida Rusconi & Neela H Yennawar & Mark Eppinger & Andrew D Patterson & Edward G Dudley, 2017. "Identification of a mouse Lactobacillus johnsonii strain with deconjugase activity against the FXR antagonist T-β-MCA," PLOS ONE, Public Library of Science, vol. 12(9), pages 1-15, September.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-49093-4. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.