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Immunization with V987H-stabilized Spike glycoprotein protects K18-hACE2 mice and golden Syrian hamsters upon SARS-CoV-2 infection

Author

Listed:
  • Carlos Ávila-Nieto

    (Campus Can Ruti)

  • Júlia Vergara-Alert

    (Campus de la Universitat Autònoma de Barcelona (UAB)
    Campus de la Universitat Autònoma de Barcelona (UAB))

  • Pep Amengual-Rigo

    (Barcelona Supercomputing Center (BSC))

  • Erola Ainsua-Enrich

    (Campus Can Ruti)

  • Marco Brustolin

    (Campus de la Universitat Autònoma de Barcelona (UAB)
    Campus de la Universitat Autònoma de Barcelona (UAB)
    Institute of Tropical Medicine)

  • María Luisa Rodríguez de la Concepción

    (Campus Can Ruti)

  • Núria Pedreño-Lopez

    (Campus Can Ruti)

  • Jordi Rodon

    (Campus de la Universitat Autònoma de Barcelona (UAB)
    Campus de la Universitat Autònoma de Barcelona (UAB))

  • Victor Urrea

    (Campus Can Ruti)

  • Edwards Pradenas

    (Campus Can Ruti)

  • Silvia Marfil

    (Campus Can Ruti)

  • Ester Ballana

    (Campus Can Ruti
    Campus Can Ruit
    CIBERINFEC. ISCIII)

  • Eva Riveira-Muñoz

    (Campus Can Ruti)

  • Mònica Pérez

    (Campus de la Universitat Autònoma de Barcelona (UAB)
    Campus de la Universitat Autònoma de Barcelona (UAB))

  • Núria Roca

    (Campus de la Universitat Autònoma de Barcelona (UAB)
    Campus de la Universitat Autònoma de Barcelona (UAB))

  • Ferran Tarrés-Freixas

    (Campus Can Ruti
    Campus de la Universitat Autònoma de Barcelona (UAB)
    Campus de la Universitat Autònoma de Barcelona (UAB))

  • Guillermo Cantero

    (Campus de la Universitat Autònoma de Barcelona (UAB))

  • Anna Pons-Grífols

    (Campus Can Ruti)

  • Carla Rovirosa

    (Campus Can Ruti)

  • Carmen Aguilar-Gurrieri

    (Campus Can Ruti)

  • Raquel Ortiz

    (Campus Can Ruti)

  • Ana Barajas

    (Campus Can Ruti)

  • Benjamin Trinité

    (Campus Can Ruti)

  • Rosalba Lepore

    (Barcelona Supercomputing Center (BSC))

  • Jordana Muñoz-Basagoiti

    (Campus Can Ruti)

  • Daniel Perez-Zsolt

    (Campus Can Ruti)

  • Nuria Izquierdo-Useros

    (Campus Can Ruti
    Campus Can Ruit
    CIBERINFEC. ISCIII)

  • Alfonso Valencia

    (Barcelona Supercomputing Center (BSC)
    Faculty of Medicine. University of Vic—Central University of Catalonia (UVic—UCC))

  • Julià Blanco

    (Campus Can Ruti
    Campus Can Ruit
    CIBERINFEC. ISCIII
    Faculty of Medicine. University of Vic—Central University of Catalonia (UVic—UCC))

  • Victor Guallar

    (Barcelona Supercomputing Center (BSC)
    Catalan Institution for Research and Advanced Studies)

  • Bonaventura Clotet

    (Campus Can Ruti
    Campus Can Ruit
    CIBERINFEC. ISCIII
    Faculty of Medicine. University of Vic—Central University of Catalonia (UVic—UCC))

  • Joaquim Segalés

    (Campus de la Universitat Autònoma de Barcelona (UAB)
    Universitat Autonoma de Barcelona. Bellaterra
    Bellaterra)

  • Jorge Carrillo

    (Campus Can Ruti
    Campus Can Ruit
    CIBERINFEC. ISCIII)

Abstract

Safe and effective severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines are crucial to fight against the coronavirus disease 2019 pandemic. Most vaccines are based on a mutated version of the Spike glycoprotein [K986P/V987P (S-2P)] with improved stability, yield and immunogenicity. However, S-2P is still produced at low levels. Here, we describe the V987H mutation that increases by two-fold the production of the recombinant Spike and the exposure of the receptor binding domain (RBD). S-V987H immunogenicity is similar to S-2P in mice and golden Syrian hamsters (GSH), and superior to a monomeric RBD. S-V987H immunization confer full protection against severe disease in K18-hACE2 mice and GSH upon SARS-CoV-2 challenge (D614G or B.1.351 variants). Furthermore, S-V987H immunized K18-hACE2 mice show a faster tissue viral clearance than RBD- or S-2P-vaccinated animals challenged with D614G, B.1.351 or Omicron BQ1.1 variants. Thus, S-V987H protein might be considered for future SARS-CoV-2 vaccines development.

Suggested Citation

  • Carlos Ávila-Nieto & Júlia Vergara-Alert & Pep Amengual-Rigo & Erola Ainsua-Enrich & Marco Brustolin & María Luisa Rodríguez de la Concepción & Núria Pedreño-Lopez & Jordi Rodon & Victor Urrea & Edwar, 2024. "Immunization with V987H-stabilized Spike glycoprotein protects K18-hACE2 mice and golden Syrian hamsters upon SARS-CoV-2 infection," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46714-w
    DOI: 10.1038/s41467-024-46714-w
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    References listed on IDEAS

    as
    1. Jarek Juraszek & Lucy Rutten & Sven Blokland & Pascale Bouchier & Richard Voorzaat & Tina Ritschel & Mark J. G. Bakkers & Ludovic L. R. Renault & Johannes P. M. Langedijk, 2021. "Stabilizing the closed SARS-CoV-2 spike trimer," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    2. Florian Krammer, 2020. "SARS-CoV-2 vaccines in development," Nature, Nature, vol. 586(7830), pages 516-527, October.
    3. Fan Wu & Su Zhao & Bin Yu & Yan-Mei Chen & Wen Wang & Zhi-Gang Song & Yi Hu & Zhao-Wu Tao & Jun-Hua Tian & Yuan-Yuan Pei & Ming-Li Yuan & Yu-Ling Zhang & Fa-Hui Dai & Yi Liu & Qi-Min Wang & Jiao-Jiao , 2020. "A new coronavirus associated with human respiratory disease in China," Nature, Nature, vol. 579(7798), pages 265-269, March.
    4. Fan Wu & Su Zhao & Bin Yu & Yan-Mei Chen & Wen Wang & Zhi-Gang Song & Yi Hu & Zhao-Wu Tao & Jun-Hua Tian & Yuan-Yuan Pei & Ming-Li Yuan & Yu-Ling Zhang & Fa-Hui Dai & Yi Liu & Qi-Min Wang & Jiao-Jiao , 2020. "Author Correction: A new coronavirus associated with human respiratory disease in China," Nature, Nature, vol. 580(7803), pages 7-7, April.
    5. Weina Sun & Yonghong Liu & Fatima Amanat & Irene González-Domínguez & Stephen McCroskery & Stefan Slamanig & Lynda Coughlan & Victoria Rosado & Nicholas Lemus & Sonia Jangra & Raveen Rathnasinghe & Mi, 2021. "A Newcastle disease virus expressing a stabilized spike protein of SARS-CoV-2 induces protective immune responses," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    6. Kizzmekia S. Corbett & Darin K. Edwards & Sarah R. Leist & Olubukola M. Abiona & Seyhan Boyoglu-Barnum & Rebecca A. Gillespie & Sunny Himansu & Alexandra Schäfer & Cynthia T. Ziwawo & Anthony T. DiPia, 2020. "SARS-CoV-2 mRNA vaccine design enabled by prototype pathogen preparedness," Nature, Nature, vol. 586(7830), pages 567-571, October.
    7. Sin Fun Sia & Li-Meng Yan & Alex W. H. Chin & Kevin Fung & Ka-Tim Choy & Alvina Y. L. Wong & Prathanporn Kaewpreedee & Ranawaka A. P. M. Perera & Leo L. M. Poon & John M. Nicholls & Malik Peiris & Hui, 2020. "Pathogenesis and transmission of SARS-CoV-2 in golden hamsters," Nature, Nature, vol. 583(7818), pages 834-838, July.
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