IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-38399-4.html
   My bibliography  Save this article

High-resolution discrimination of homologous and isomeric proteinogenic amino acids in nanopore sensors with ultrashort single-walled carbon nanotubes

Author

Listed:
  • Weichao Peng

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Shuaihu Yan

    (University of Chinese Academy of Sciences
    Chinese Academy of Sciences)

  • Ke Zhou

    (Chinese Academy of Sciences)

  • Hai-Chen Wu

    (University of Chinese Academy of Sciences
    Chinese Academy of Sciences)

  • Lei Liu

    (Chinese Academy of Sciences)

  • Yuliang Zhao

    (Chinese Academy of Sciences
    National Center for Nanoscience and Technology)

Abstract

The hollow and tubular structure of single-walled carbon nanotubes (SWCNTs) makes them ideal candidates for making nanopores. However, the heterogeneity of SWCNTs hinders the fabrication of robust and reproducible carbon-based nanopore sensors. Here we develop a modified density gradient ultracentrifugation approach to separate ultrashort (≈5-10 nm) SWCNTs with a narrow conductance range and construct high-resolution nanopore sensors with those tubes inserted in lipid bilayers. By conducting ionic current recordings and fluorescent imaging of Ca2+ flux through different nanopores, we prove that the ion mobilities in SWCNT nanopores are 3-5 times higher than the bulk mobility. Furthermore, we employ SWCNT nanopores to discriminate homologue or isomeric proteinogenic amino acids, which are challenging tasks for other nanopore sensors. These successes, coupled with the building of SWCNT nanopore arrays, may constitute a crucial part of the recently burgeoning protein sequencing technologies.

Suggested Citation

  • Weichao Peng & Shuaihu Yan & Ke Zhou & Hai-Chen Wu & Lei Liu & Yuliang Zhao, 2023. "High-resolution discrimination of homologous and isomeric proteinogenic amino acids in nanopore sensors with ultrashort single-walled carbon nanotubes," Nature Communications, Nature, vol. 14(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38399-4
    DOI: 10.1038/s41467-023-38399-4
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-38399-4
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-38399-4?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. G. Hummer & J. C. Rasaiah & J. P. Noworyta, 2001. "Water conduction through the hydrophobic channel of a carbon nanotube," Nature, Nature, vol. 414(6860), pages 188-190, November.
    2. Jia Geng & Kyunghoon Kim & Jianfei Zhang & Artur Escalada & Ramya Tunuguntla & Luis R. Comolli & Frances I. Allen & Anna V. Shnyrova & Kang Rae Cho & Dayannara Munoz & Y. Morris Wang & Costas P. Grigo, 2014. "Stochastic transport through carbon nanotubes in lipid bilayers and live cell membranes," Nature, Nature, vol. 514(7524), pages 612-615, October.
    3. Hagan Bayley & Paul S. Cremer, 2001. "Stochastic sensors inspired by biology," Nature, Nature, vol. 413(6852), pages 226-230, September.
    4. Yunhyun Lee & Hyun Jung Kim & Dong-Kwon Kim, 2020. "Power Generation from Concentration Gradient by Reverse Electrodialysis in Anisotropic Nanoporous Anodic Aluminum Oxide Membranes," Energies, MDPI, vol. 13(4), pages 1-15, February.
    5. Lei Liu & Chun Yang & Kai Zhao & Jingyuan Li & Hai-Chen Wu, 2013. "Ultrashort single-walled carbon nanotubes in a lipid bilayer as a new nanopore sensor," Nature Communications, Nature, vol. 4(1), pages 1-8, December.
    6. Jhinhwan Lee & H. Kim & S.-J. Kahng & G. Kim & Y.-W. Son & J. Ihm & H. Kato & Z. W. Wang & T. Okazaki & H. Shinohara & Young Kuk, 2002. "Bandgap modulation of carbon nanotubes by encapsulated metallofullerenes," Nature, Nature, vol. 415(6875), pages 1005-1008, February.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Köhler, Mateus Henrique & Bordin, José Rafael & da Silva, Leandro B. & Barbosa, Marcia C., 2018. "Structure and dynamics of water inside hydrophobic and hydrophilic nanotubes," Physica A: Statistical Mechanics and its Applications, Elsevier, vol. 490(C), pages 331-337.
    2. Mian Muhammad-Ahson Aslam & Hsion-Wen Kuo & Walter Den & Muhammad Usman & Muhammad Sultan & Hadeed Ashraf, 2021. "Functionalized Carbon Nanotubes (CNTs) for Water and Wastewater Treatment: Preparation to Application," Sustainability, MDPI, vol. 13(10), pages 1-54, May.
    3. Tongcai Liu & Shaoze Xiao & Nan Li & Jiabin Chen & Xuefei Zhou & Yajie Qian & Ching-Hua Huang & Yalei Zhang, 2023. "Water decontamination via nonradical process by nanoconfined Fenton-like catalysts," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    4. Simon Sau Yin Law & Geoffrey Liou & Yukiko Nagai & Joan Giménez-Dejoz & Ayaka Tateishi & Kousuke Tsuchiya & Yutaka Kodama & Tsuyohiko Fujigaya & Keiji Numata, 2022. "Polymer-coated carbon nanotube hybrids with functional peptides for gene delivery into plant mitochondria," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    5. Ferlin Robinson & Majid Shahbabaei & Daejoong Kim, 2019. "Deformation Effect on Water Transport through Nanotubes," Energies, MDPI, vol. 12(23), pages 1-12, November.
    6. Ruo-Xu Gu & Bert L. Groot, 2023. "Central cavity dehydration as a gating mechanism of potassium channels," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    7. Xiao-Feng Wang & Kun Xu & Xin-Rui Li & Ya-Xin Liu & Jie-Min Cheng, 2023. "Damage Effect of Amorphous Carbon Black Nanoparticle Aggregates on Model Phospholipid Membranes: Surface Charge, Exposure Concentration and Time Dependence," IJERPH, MDPI, vol. 20(4), pages 1-12, February.
    8. Ying-Ying Wang & Samuel K Lai & Conan So & Craig Schneider & Richard Cone & Justin Hanes, 2011. "Mucoadhesive Nanoparticles May Disrupt the Protective Human Mucus Barrier by Altering Its Microstructure," PLOS ONE, Public Library of Science, vol. 6(6), pages 1-7, June.
    9. Ng, Edmund Chong Jie & Kueh, Tze Cheng & Wang, Xin & Soh, Ai Kah & Hung, Yew Mun, 2021. "Anomalously enhanced thermal performance of carbon-nanotubes coated micro heat pipes," Energy, Elsevier, vol. 214(C).
    10. Guangli Liu & Bin Zhou & Jinwei Liu & Huazhang Zhao, 2020. "The Bionic Water Channel of Ultra-Short, High Affinity Carbon Nanotubes with High Water Permeability and Proton Selectivity," Sustainability, MDPI, vol. 13(1), pages 1-13, December.
    11. Lim, Melvin C.G. & Pei, Q.X. & Zhong, Z.W., 2008. "Translocation of DNA oligonucleotide through carbon nanotube channels under induced pressure difference," Physica A: Statistical Mechanics and its Applications, Elsevier, vol. 387(13), pages 3111-3120.
    12. Soo-Jin Han & Jin-Soo Park, 2021. "Understanding Membrane Fouling in Electrically Driven Energy Conversion Devices," Energies, MDPI, vol. 14(1), pages 1-11, January.
    13. Weiwen Xin & Jingru Fu & Yongchao Qian & Lin Fu & Xiang-Yu Kong & Teng Ben & Lei Jiang & Liping Wen, 2022. "Biomimetic KcsA channels with ultra-selective K+ transport for monovalent ion sieving," Nature Communications, Nature, vol. 13(1), pages 1-11, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38399-4. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.