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Augmented CO2 tolerance by expressing a single H+-pump enables microalgal valorization of industrial flue gas

Author

Listed:
  • Hong Il Choi

    (Korea University)

  • Sung-Won Hwang

    (Korea University)

  • Jongrae Kim

    (Hanyang University)

  • Byeonghyeok Park

    (Korea University)

  • EonSeon Jin

    (Hanyang University)

  • In-Geol Choi

    (Korea University)

  • Sang Jun Sim

    (Korea University)

Abstract

Microalgae can accumulate various carbon-neutral products, but their real-world applications are hindered by their CO2 susceptibility. Herein, the transcriptomic changes in a model microalga, Chlamydomonas reinhardtii, in a high-CO2 milieu (20%) are evaluated. The primary toxicity mechanism consists of aberrantly low expression of plasma membrane H+-ATPases (PMAs) accompanied by intracellular acidification. Our results demonstrate that the expression of a universally expressible PMA in wild-type strains makes them capable of not only thriving in acidity levels that they usually cannot survive but also exhibiting 3.2-fold increased photoautotrophic production against high CO2 via maintenance of a higher cytoplasmic pH. A proof-of-concept experiment involving cultivation with toxic flue gas (13 vol% CO2, 20 ppm NOX, and 32 ppm SOX) shows that the production of CO2-based bioproducts by the strain is doubled compared with that by the wild-type, implying that this strategy potentially enables the microalgal valorization of CO2 in industrial exhaust.

Suggested Citation

  • Hong Il Choi & Sung-Won Hwang & Jongrae Kim & Byeonghyeok Park & EonSeon Jin & In-Geol Choi & Sang Jun Sim, 2021. "Augmented CO2 tolerance by expressing a single H+-pump enables microalgal valorization of industrial flue gas," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26325-5
    DOI: 10.1038/s41467-021-26325-5
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    References listed on IDEAS

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    1. Bjørn P. Pedersen & Morten J. Buch-Pedersen & J. Preben Morth & Michael G. Palmgren & Poul Nissen, 2007. "Crystal structure of the plasma membrane proton pump," Nature, Nature, vol. 450(7172), pages 1111-1114, December.
    2. Zheng, Yubin & Li, Tingting & Yu, Xiaochen & Bates, Philip D. & Dong, Tao & Chen, Shulin, 2013. "High-density fed-batch culture of a thermotolerant microalga Chlorella sorokiniana for biofuel production," Applied Energy, Elsevier, vol. 108(C), pages 281-287.
    3. Judd, S.J. & Al Momani, F.A.O. & Znad, H. & Al Ketife, A.M.D., 2017. "The cost benefit of algal technology for combined CO2 mitigation and nutrient abatement," Renewable and Sustainable Energy Reviews, Elsevier, vol. 71(C), pages 379-387.
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    1. Choi, Hong Il & Sung, Young Joon & Hong, Min Eui & Han, Jonghee & Min, Byoung Koun & Sim, Sang Jun, 2022. "Reconsidering the potential of direct microalgal biomass utilization as end-products: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 155(C).

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