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Co-production of biohydrogen and biodiesel from different microalgae by ultrasound, anaerobic fermentation and lipid extraction

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  • Shi, Yue
  • Wang, Ruxian
  • Tian, Encai
  • Xing, Yuchen
  • Li, Fan
  • Lu, Zheng

Abstract

Microalgae as biomass feedstocks vary greatly in their physicochemical properties and chemical composition, and the effect of microalgal feedstock properties in stepwise energy production has rarely been reported. In this study, five typical microalgae, namely Nannochloropsis sp., Chlorella sp., Schizochytrium sp., Thalassiosira sp., and Dunaliella sp., were used as feedstock, and ultrasound was used to assist in the co-production of biohydrogen and biodiesel. Different microalgae exhibited different fermentation types in anaerobic fermentation and their hydrogen production was highly correlated with carbohydrate content. The highest hydrogen production potential was obtained from Dunaliella sp. with a hydrogen yield of 2.87 mL/gTS. The highest lipid yield of 29.65 % was obtained from Schizochytrium sp. Anaerobic fermentation followed by lipid extraction resulted in a 2.3-fold increase in absolute lipid yields from Nannochloropsis sp. Compared to direct lipid extraction. Using the “ultrasound-anaerobic fermentation-lipid extraction” production route, all five microalgae species achieved maximum biohydrogen and biodiesel yields. After fermentation, the microbial community system was more specialised, forming a complex system dominated by Clostridium. Both mechanical effects of ultrasound and microbial hydrogenation altered the composition of the five microalgal biodiesel species and increased the saturated fatty acid percentage, but the effect of anaerobic fermentation was stronger.

Suggested Citation

  • Shi, Yue & Wang, Ruxian & Tian, Encai & Xing, Yuchen & Li, Fan & Lu, Zheng, 2026. "Co-production of biohydrogen and biodiesel from different microalgae by ultrasound, anaerobic fermentation and lipid extraction," Renewable Energy, Elsevier, vol. 258(C).
    • Handle: RePEc:eee:renene:v:258:y:2026:i:c:s0960148125026783
    DOI: 10.1016/j.renene.2025.125014
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    References listed on IDEAS

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