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Catalytic performance of immobilized thermostable fungal lipases in Ethanolysis of buriti and sunflower oils for sustainable biodiesel production

Author

Listed:
  • Rocha, Mariana de Souza
  • de Andrades, D.
  • Vici, Ana Claudia
  • Gonçalves, Enrico Cerioni Spiropulos
  • Vieira, Andressa Tironi
  • Batista, Antonio Carlos Ferreira
  • Faria, Anízio Márcio
  • Polizeli, Maria de Lourdes Teixeira de Moraes

Abstract

This study identified fungal strains with high lipase production and transesterification capabilities. The thermophilic Scytalidium thermophilum and the mesophilic Aspergillus phoenicis showed the highest enzymatic activity. Optimal lipase production was achieved using SR medium with 1 % sunflower oil for A. phoenicis and 1 % buriti oil for S. thermophilum. Lipases from both fungi were immobilized on various supports. The S. thermophilum lipase immobilized on Duolite (StDL) remained stable at 50 °C in solvents like cyclohexane, ethanol, and acetone, retaining over 70 % activity after 24 h. The A. phoenicis lipase immobilized on Octyl-Sepharose (ApOctyl) retained 93 % activity at 50 °C after 48 h in cyclohexane. StDL was selected for biodiesel synthesis using oils from soybean, sunflower, açai, and buriti. Transesterification with cyclohexane and ethanol yielded 54 % ethyl esters from sunflower oil and 34 % from buriti oil. Buriti oil produced 77 % ethyl oleate, açai 51 %, and soybean 37 %. Biodiesel met EN 14214 and partially met ASTM D6751 standards, with viscosities of 4.27–4.60 mm2/s and densities between 0.860 and 0.880 g cm−3. Only soybean and açai biodiesel met the ASTM density range. These results highlight the promise of fungal immobilized lipases for producing biodiesel from native Brazilian oils and food byproducts.

Suggested Citation

  • Rocha, Mariana de Souza & de Andrades, D. & Vici, Ana Claudia & Gonçalves, Enrico Cerioni Spiropulos & Vieira, Andressa Tironi & Batista, Antonio Carlos Ferreira & Faria, Anízio Márcio & Polizeli, Mar, 2026. "Catalytic performance of immobilized thermostable fungal lipases in Ethanolysis of buriti and sunflower oils for sustainable biodiesel production," Renewable Energy, Elsevier, vol. 256(PA).
    • Handle: RePEc:eee:renene:v:256:y:2026:i:pa:s0960148125015484
    DOI: 10.1016/j.renene.2025.123884
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    References listed on IDEAS

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    1. Osman, Ahmed I. & Qasim, Umair & Jamil, Farrukh & Al-Muhtaseb, Ala'a H. & Jrai, Ahmad Abu & Al-Riyami, Mohammed & Al-Maawali, Suhaib & Al-Haj, Lamya & Al-Hinai, Amer & Al-Abri, Mohammed & Inayat, Abra, 2021. "Bioethanol and biodiesel: Bibliometric mapping, policies and future needs," Renewable and Sustainable Energy Reviews, Elsevier, vol. 152(C).
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    3. Héctor Sánchez-Morán & Joel L. Kaar & Daniel K. Schwartz, 2024. "Supra-biological performance of immobilized enzymes enabled by chaperone-like specific non-covalent interactions," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
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