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Biochemical and genetic changes revealing the enhanced lipid accumulation in Desmodesmus sp. mutated by atmospheric and room temperature plasma

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  • Li, Pengfei
  • Sun, Xin
  • Sun, Zhe
  • Huang, Feng
  • Wei, Wenqian
  • Liu, Xingshe
  • Liu, Yongjun
  • Deng, Linyu
  • Cheng, Zhiwen

Abstract

This research reveals biochemical and genetic changes for enhanced lipid production in Desmodesmus sp. mutated using atmospheric and room temperature plasma. Three lipid-rich mutant strains were selected by the special screening method, decided by the specific growth rate, relative fluorescence and the combination. There were no significant differences in genetic stability under 3-generation cultivation (p > 0.05). The highest lipid production and biomass increased by >100% and >15% compared to original strain, respectively. The activities of lipid enzymes, including acetyl-CoA carboxylase and diacylglycerol acyltransferases, responded to similar trends with lipid accumulation. Functional genes classification of mutant strains showed a great ratio in genetic information process (33.51%) and metabolism pathway (28.74%). More than 80% genes were not significant in lipid-related metabolisms and total 27 significant genes were analyzed, signifying similar genetic changes in mutant strains. Finally the establishing simplified diagram explained high lipid accumulation based on typical enzymes for fatty acid synthesis by upregulated genes, ACACA, fabF, fabG, ACSL, as well as triacylglycerol synthesis by upregulated genes, glpk, GPAT1_2, plsC, PDAT, dgat 1 and MGAT2. Lipid-rich strains performed a positive effect on lipid accumulation. These relative regulated genes and analysis might provide the control points for high lipid production in energy consumption.

Suggested Citation

  • Li, Pengfei & Sun, Xin & Sun, Zhe & Huang, Feng & Wei, Wenqian & Liu, Xingshe & Liu, Yongjun & Deng, Linyu & Cheng, Zhiwen, 2021. "Biochemical and genetic changes revealing the enhanced lipid accumulation in Desmodesmus sp. mutated by atmospheric and room temperature plasma," Renewable Energy, Elsevier, vol. 172(C), pages 368-381.
    • Handle: RePEc:eee:renene:v:172:y:2021:i:c:p:368-381
    DOI: 10.1016/j.renene.2021.03.048
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    References listed on IDEAS

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    1. Sun, Han & Wu, Tao & Chen, Stephenie Hiu Yuet & Ren, Yuanyuan & Yang, Shufang & Huang, Junchao & Mou, Haijin & Chen, Feng, 2021. "Powerful tools for productivity improvements in microalgal production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 152(C).

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